Component

Dlat overexpression in neonatal rat ventricular cardiomyocytes

Experimental overexpression in a rat-cell host; do not infer the adenoviral insert species where not specified.

7 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

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What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Dlat overexpression increased mitochondrial protein acetylation in neonatal rat cardiomyocytes.

    Dlat overexpression in neonatal rat ventricular cardiomyocytes → Rat cardiac mitochondrial protein lysine acetylation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-mitochondrial-protein-acetylation
    evidence_location
    Mitochondrial immunoblot; Figure 3G/H
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    rat-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Other FAO proteins showed increased acetylation, but direct catalytic modification of each was not established.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    Dlat overexpression increased mitochondrial protein acetylation in neonatal rat cardiomyocytes.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 30, "text_sha256": "c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    6 overall; 3 per individual protein panel
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 232–247

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    Dlat overexpression increased mitochondrial protein acetylation in neonatal rat cardiomyocytes. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Mitochondrial immunoblot; Figure 3G/H endpoint: rat-mitochondrial-protein-acetylation exposure: rat-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Other FAO proteins showed increased acetylation, but direct catalytic modification of each was not established. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 30, "text_sha256": "c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}] plain_language: Dlat overexpression increased mitochondrial protein acetylation in neonatal rat cardiomyocytes. sample_size: 6 overall; 3 per individual protein panel
    Complete structured claim and evidence

  2. Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes.

    Dlat overexpression in neonatal rat ventricular cardiomyocytes → Rat cardiac fatty-acid oxidation capacity source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiac-fao
    evidence_location
    Seahorse palmitate oxidation stress test; Figure 3L–N
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    rat-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. OCR is a respiratory readout rather than a complete FAO flux balance. Etomoxir concentration was not specified in the reviewed methods.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 30, "text_sha256": "c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 90, "text_sha256": "9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700", "xml_element_id": "Par40"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 biological replicates
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 196–211

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Seahorse palmitate oxidation stress test; Figure 3L–N endpoint: rat-cardiac-fao exposure: rat-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. OCR is a respiratory readout rather than a complete FAO flux balance. Etomoxir concentration was not specified in the reviewed methods. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 30, "text_sha256": "c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 90, "text_sha256": "9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700", "xml_element_id": "Par40"}] plain_language: Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes. sample_size: 4 biological replicates
    Complete structured claim and evidence

  3. Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification.

    Dlat overexpression in neonatal rat ventricular cardiomyocytes → Rat cardiomyocyte glycolysis-associated extracellular acidification source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiomyocyte-glycolytic-acidification
    evidence_location
    Seahorse ECAR; Figure 3P–R
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    rat-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. ECAR is an assay readout, not a direct measure of PDH flux. The molecular cause remains unresolved.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 30, "text_sha256": "c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 90, "text_sha256": "9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700", "xml_element_id": "Par40"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 250–264

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Seahorse ECAR; Figure 3P–R endpoint: rat-cardiomyocyte-glycolytic-acidification exposure: rat-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. ECAR is an assay readout, not a direct measure of PDH flux. The molecular cause remains unresolved. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 30, "text_sha256": "c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 90, "text_sha256": "9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700", "xml_element_id": "Par40"}] plain_language: Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification.
    Complete structured claim and evidence

  4. Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA.

    Dlat overexpression in neonatal rat ventricular cardiomyocytes → Cellular fatty acid oxidation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    fatty-acid-oxidation
    evidence_location
    13C-palmitate tracing; Figure 6L
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    rat-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Reported metabolite-labeling pattern supports impaired breakdown; it does not itself establish every kinetic step or isolate HADHA from other Dlat effects.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 44, "text_sha256": "e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621", "xml_element_id": "Par19"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 46, "text_sha256": "d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 per group
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 214–229

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: 13C-palmitate tracing; Figure 6L endpoint: fatty-acid-oxidation exposure: rat-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Reported metabolite-labeling pattern supports impaired breakdown; it does not itself establish every kinetic step or isolate HADHA from other Dlat effects. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 44, "text_sha256": "e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621", "xml_element_id": "Par19"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 46, "text_sha256": "d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff", "xml_element_id": null}] plain_language: Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA. sample_size: 4 per group
    Complete structured claim and evidence

  5. Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes.

    Dlat overexpression in neonatal rat ventricular cardiomyocytes → Rat cardiomyocyte lipid-droplet accumulation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiac-lipid-droplets
    evidence_location
    BODIPY staining; Figure 3O
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    rat-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 267–281

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: BODIPY staining; Figure 3O endpoint: rat-cardiac-lipid-droplets exposure: rat-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}] plain_language: Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes.
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.

    HADHA K728R experimental construct → HADHA-attributed activity in lysate assay source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    hadha-attributed-lysate-activity
    evidence_location
    Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    hadha-k728r-construct
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    3 per group
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 160–175

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H endpoint: hadha-attributed-lysate-activity exposure: hadha-k728r-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}] plain_language: In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not. sample_size: 3 per group
    Complete structured claim and evidence

  2. WY14643 treatment restored fatty-acid-supported respiration in Dlat-overexpressing rat cardiomyocytes.

    WY14643 experimental PPAR-alpha agonist → Rat cardiac fatty-acid oxidation capacity source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiac-fao
    evidence_location
    Supplementary Figure S9
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    wy14643
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. PPAR-alpha agonist experiment; dose and duration not specified in the reviewed text. It does not establish selective action on HADHA.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    WY14643 treatment restored fatty-acid-supported respiration in Dlat-overexpressing rat cardiomyocytes.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 318–332

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    WY14643 treatment restored fatty-acid-supported respiration in Dlat-overexpressing rat cardiomyocytes. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Supplementary Figure S9 endpoint: rat-cardiac-fao exposure: wy14643 limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. PPAR-alpha agonist experiment; dose and duration not specified in the reviewed text. It does not establish selective action on HADHA. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}] plain_language: WY14643 treatment restored fatty-acid-supported respiration in Dlat-overexpressing rat cardiomyocytes.
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