Component

HADHA K728R experimental construct

Study-specific entity. Experimental conditions and evidence limits remain on each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

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What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. The K728R HADHA construct resisted the Dlat-induced increase in acetyl-lysine signal in HEK293 experiments.

    HADHA K728R experimental construct → Acetyl-lysine signal on experimental HADHA constructs source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Immunoprecipitated tagged protein
    dose
    Construct amounts not specified in reviewed text
    duration
    Not specified in reviewed text
    endpoint
    hadha-construct-lysine-acetylation
    evidence_location
    Flag immunoprecipitation/acetyl-lysine blot; Figure 7D/F
    experimental_model
    Flag-HADHA point-mutant/combined-mutant transfection
    exposure
    hadha-k728r-construct
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. K728R removes the lysine: this blot does not measure acetylation at residue 728. Other-site and structural effects remain possible; peer-review response pp.9–10.
    organism
    Homo sapiens HEK293 host; construct sequence species not assumed from host
    plain_language
    The K728R HADHA construct resisted the Dlat-induced increase in acetyl-lysine signal in HEK293 experiments.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM3_ESM.pdf", "location": "PDF pages 9-10 and 16-17; concern and subsequent additional controls", "sha256": "79ca0576c4b70ef33a468724c6ec9f2ed75041428536ec0e2444561ee575b865"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 per group
    tissue_or_cell_type
    HEK293 cells

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 106–121

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Flag-HADHA point-mutant/combined-mutant transfection · source_derived_draft · unverified_draft

    The K728R HADHA construct resisted the Dlat-induced increase in acetyl-lysine signal in HEK293 experiments. organism: Homo sapiens HEK293 host; construct sequence species not assumed from host tissue_or_cell_type: HEK293 cells experimental_model: Flag-HADHA point-mutant/combined-mutant transfection compartment: Immunoprecipitated tagged protein dose: Construct amounts not specified in reviewed text duration: Not specified in reviewed text primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Flag immunoprecipitation/acetyl-lysine blot; Figure 7D/F endpoint: hadha-construct-lysine-acetylation exposure: hadha-k728r-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. K728R removes the lysine: this blot does not measure acetylation at residue 728. Other-site and structural effects remain possible; peer-review response pp.9–10. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM3_ESM.pdf", "location": "PDF pages 9-10 and 16-17; concern and subsequent additional controls", "sha256": "79ca0576c4b70ef33a468724c6ec9f2ed75041428536ec0e2444561ee575b865"}] plain_language: The K728R HADHA construct resisted the Dlat-induced increase in acetyl-lysine signal in HEK293 experiments. sample_size: 4 per group
    Complete structured claim and evidence

  2. In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.

    HADHA K728R experimental construct → HADHA-attributed activity in lysate assay source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    hadha-attributed-lysate-activity
    evidence_location
    Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    hadha-k728r-construct
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    3 per group
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 160–175

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H endpoint: hadha-attributed-lysate-activity exposure: hadha-k728r-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}] plain_language: In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not. sample_size: 3 per group
    Complete structured claim and evidence

  3. K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.

    HADHA K728R experimental construct → Rat cardiomyocyte lipid-droplet accumulation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiac-lipid-droplets
    evidence_location
    BODIPY microscopy; Figure 7J/K
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    hadha-k728r-construct
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Cell-model phenotype; not proof of mouse-heart or endothelial barrier rescue.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    8 biological replicates
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 178–193

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: BODIPY microscopy; Figure 7J/K endpoint: rat-cardiac-lipid-droplets exposure: hadha-k728r-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Cell-model phenotype; not proof of mouse-heart or endothelial barrier rescue. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}] plain_language: K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct. sample_size: 8 biological replicates
    Complete structured claim and evidence

  4. Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct.

    HADHA K728R experimental construct → Acetyl-lysine signal on experimental HADHA constructs source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    In vitro reaction, not an intact-cell compartment
    dose
    Acetyl-CoA present/absent controls; concentrations and incubation details incompletely specified
    duration
    Not specified in the reviewed methods
    endpoint
    hadha-construct-lysine-acetylation
    evidence_location
    Figure 7E
    experimental_model
    Recombinant-protein acetylation assay
    exposure
    hadha-k728r-construct
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A construct-dependent result, not direct residue-specific occupancy quantification or a mouse-heart mutant experiment.
    organism
    Recombinant mouse Dlat; HADHA construct sequence species unresolved in reviewed methods
    plain_language
    Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    4 biological replicates
    tissue_or_cell_type
    Purified-protein reaction

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 142–157

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Recombinant-protein acetylation assay · source_derived_draft · unverified_draft

    Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct. organism: Recombinant mouse Dlat; HADHA construct sequence species unresolved in reviewed methods tissue_or_cell_type: Purified-protein reaction experimental_model: Recombinant-protein acetylation assay compartment: In vitro reaction, not an intact-cell compartment dose: Acetyl-CoA present/absent controls; concentrations and incubation details incompletely specified duration: Not specified in the reviewed methods primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Figure 7E endpoint: hadha-construct-lysine-acetylation exposure: hadha-k728r-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A construct-dependent result, not direct residue-specific occupancy quantification or a mouse-heart mutant experiment. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}] plain_language: Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct. sample_size: 4 biological replicates
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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