Component

Rat cardiomyocyte lipid-droplet accumulation

Study-specific entity. Experimental conditions and evidence limits remain on each linked claim.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

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What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.

    HADHA K728R experimental construct → Rat cardiomyocyte lipid-droplet accumulation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiac-lipid-droplets
    evidence_location
    BODIPY microscopy; Figure 7J/K
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    hadha-k728r-construct
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Cell-model phenotype; not proof of mouse-heart or endothelial barrier rescue.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    sample_size
    8 biological replicates
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 178–193

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: BODIPY microscopy; Figure 7J/K endpoint: rat-cardiac-lipid-droplets exposure: hadha-k728r-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Cell-model phenotype; not proof of mouse-heart or endothelial barrier rescue. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}] plain_language: K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct. sample_size: 8 biological replicates
    Complete structured claim and evidence

  2. PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment.

    PDK4 overexpression in neonatal rat ventricular cardiomyocytes → Rat cardiomyocyte lipid-droplet accumulation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiac-lipid-droplets
    evidence_location
    Supplementary Figure S8C/D; peer-review response pp.36–37
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    rat-pdk4-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. This control weakens a simple phosphorylation-only explanation but does not exclude every contribution from PDH metabolism.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM3_ESM.pdf", "location": "PDF pages 36-37", "sha256": "79ca0576c4b70ef33a468724c6ec9f2ed75041428536ec0e2444561ee575b865"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 301–315

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Supplementary Figure S8C/D; peer-review response pp.36–37 endpoint: rat-cardiac-lipid-droplets exposure: rat-pdk4-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. This control weakens a simple phosphorylation-only explanation but does not exclude every contribution from PDH metabolism. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}, {"cache": "artifacts/dlat-curation/41467_2026_70703_MOESM3_ESM.pdf", "location": "PDF pages 36-37", "sha256": "79ca0576c4b70ef33a468724c6ec9f2ed75041428536ec0e2444561ee575b865"}] plain_language: PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment.
    Complete structured claim and evidence

  3. Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes.

    Dlat overexpression in neonatal rat ventricular cardiomyocytes → Rat cardiomyocyte lipid-droplet accumulation source_derived_draftungraded
    Experimental context and source evidence
    access_level
    full_text_and_supplement_review
    compartment
    Mitochondria or cell lysate, depending on assay
    dose
    Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
    duration
    Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
    endpoint
    rat-cardiac-lipid-droplets
    evidence_location
    BODIPY staining; Figure 3O
    experimental_model
    Adenoviral/cell perturbation study
    exposure
    rat-dlat-overexpression
    limitations
    Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects.
    organism
    Rattus norvegicus host cells; construct species may be unspecified
    plain_language
    Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes.
    primary_locator
    [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}]
    primary_references
    https://doi.org/10.1038/s41467-026-70703-w
    tissue_or_cell_type
    Neonatal rat ventricular cardiomyocytes (NRVCMs)

    DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 267–281

    AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft

    Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: BODIPY staining; Figure 3O endpoint: rat-cardiac-lipid-droplets exposure: rat-dlat-overexpression limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 31, "text_sha256": "74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0", "xml_element_id": "Par13"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 32, "text_sha256": "ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731", "xml_element_id": "Par14"}] plain_language: Dlat overexpression increased lipid-droplet accumulation in neonatal rat cardiomyocytes.
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards