{"id":"ab1e2fcb-7dad-53b0-846e-a8b4bcefcc79","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:k728r-lipid-rescue","predicate":"reduces","statement":"K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"6caaffa3-f039-5f5c-a968-5a30b0ab1c37","mechanism_event_label":"k728r lipid rescue","subject":{"id":"d693cf50-c5e0-5600-ae73-d67f0f8157e6","slug":"hadha-k728r-construct","display_name":"HADHA K728R experimental construct","entity_type_key":"protein_state"},"object":{"id":"9676d9de-8cd2-519a-aec5-215a2916ee00","slug":"rat-cardiac-lipid-droplets","display_name":"Rat cardiomyocyte lipid-droplet accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6caaffa3-f039-5f5c-a968-5a30b0ab1c37","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:k728r-lipid-rescue","event_type":"reduces","label":"k728r lipid rescue","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d693cf50-c5e0-5600-ae73-d67f0f8157e6","slug":"hadha-k728r-construct","display_name":"HADHA K728R experimental construct","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9676d9de-8cd2-519a-aec5-215a2916ee00","slug":"rat-cardiac-lipid-droplets","display_name":"Rat cardiomyocyte lipid-droplet accumulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-cardiac-lipid-droplets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"BODIPY microscopy; Figure 7J/K","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adenoviral/cell perturbation study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"hadha-k728r-construct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Cell-model phenotype; not proof of mouse-heart or endothelial barrier rescue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"8 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a366de1c-bbab-5fa2-8ced-5dead54101cd","evidence_kind":"source_excerpt","locator":"Lines 178-193","start_line":178,"end_line":193,"excerpt":"K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Adenoviral/cell perturbation study\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods\nduration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: BODIPY microscopy; Figure 7J/K\nendpoint: rat-cardiac-lipid-droplets\nexposure: hadha-k728r-construct\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Cell-model phenotype; not proof of mouse-heart or endothelial barrier rescue.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}]\nplain_language: K728R HADHA reduced Dlat-associated lipid-droplet accumulation in neonatal rat cardiomyocytes more than the wild-type construct.\nsample_size: 8 biological replicates","model_system":"Adenoviral/cell perturbation study","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}