{"id":"ee76e7a9-e0b3-593a-85fa-aa79ddaf7286","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:pdk4-lipid-null","predicate":"does_not_reproduce","statement":"PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"5724a511-220a-560a-99a5-a9380e8141e6","mechanism_event_label":"pdk4 lipid null","subject":{"id":"e1f96aa2-37f7-5f22-a7bc-84928da5a92d","slug":"rat-pdk4-overexpression","display_name":"PDK4 overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"object":{"id":"9676d9de-8cd2-519a-aec5-215a2916ee00","slug":"rat-cardiac-lipid-droplets","display_name":"Rat cardiomyocyte lipid-droplet accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5724a511-220a-560a-99a5-a9380e8141e6","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:pdk4-lipid-null","event_type":"does_not_reproduce","label":"pdk4 lipid null","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e1f96aa2-37f7-5f22-a7bc-84928da5a92d","slug":"rat-pdk4-overexpression","display_name":"PDK4 overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9676d9de-8cd2-519a-aec5-215a2916ee00","slug":"rat-cardiac-lipid-droplets","display_name":"Rat cardiomyocyte lipid-droplet accumulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-cardiac-lipid-droplets","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Supplementary Figure S8C/D; peer-review response pp.36–37","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adenoviral/cell perturbation study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"rat-pdk4-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. 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This control weakens a simple phosphorylation-only explanation but does not exclude every contribution from PDH metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 32, \"text_sha256\": \"ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731\", \"xml_element_id\": \"Par14\"}, {\"cache\": \"artifacts/dlat-curation/41467_2026_70703_MOESM3_ESM.pdf\", \"location\": \"PDF pages 36-37\", \"sha256\": \"79ca0576c4b70ef33a468724c6ec9f2ed75041428536ec0e2444561ee575b865\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d45c6b2e-295c-51af-a692-c770ecb46d47","evidence_kind":"source_excerpt","locator":"Lines 301-315","start_line":301,"end_line":315,"excerpt":"PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Adenoviral/cell perturbation study\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods\nduration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Supplementary Figure S8C/D; peer-review response pp.36–37\nendpoint: rat-cardiac-lipid-droplets\nexposure: rat-pdk4-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. This control weakens a simple phosphorylation-only explanation but does not exclude every contribution from PDH metabolism.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 32, \"text_sha256\": \"ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731\", \"xml_element_id\": \"Par14\"}, {\"cache\": \"artifacts/dlat-curation/41467_2026_70703_MOESM3_ESM.pdf\", \"location\": \"PDF pages 36-37\", \"sha256\": \"79ca0576c4b70ef33a468724c6ec9f2ed75041428536ec0e2444561ee575b865\"}]\nplain_language: PDK4 overexpression did not reproduce the reported lipid-droplet accumulation in the rat-cell control experiment.","model_system":"Adenoviral/cell perturbation study","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}