{"id":"b2e36fbe-8601-5205-82db-36d4c6c1bfea","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:k728r-activity-rescue","predicate":"preserves_assay_activity","statement":"In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"28eacd13-8d07-55b2-b6f3-e96ea8c1c9be","mechanism_event_label":"k728r activity rescue","subject":{"id":"d693cf50-c5e0-5600-ae73-d67f0f8157e6","slug":"hadha-k728r-construct","display_name":"HADHA K728R experimental construct","entity_type_key":"protein_state"},"object":{"id":"b91e004b-b413-5361-97f4-ab5695d3e6ad","slug":"hadha-attributed-lysate-activity","display_name":"HADHA-attributed activity in lysate assay","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"28eacd13-8d07-55b2-b6f3-e96ea8c1c9be","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:k728r-activity-rescue","event_type":"preserves_assay_activity","label":"k728r activity rescue","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d693cf50-c5e0-5600-ae73-d67f0f8157e6","slug":"hadha-k728r-construct","display_name":"HADHA K728R experimental construct","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b91e004b-b413-5361-97f4-ab5695d3e6ad","slug":"hadha-attributed-lysate-activity","display_name":"HADHA-attributed activity in lysate assay","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3b7fe5a1-f321-5b5d-b98c-bd6b37ddbd19","slug":"hadha-k411r-construct","display_name":"HADHA K411R experimental construct","entity_type_key":"protein_state"},"role":"negative comparator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e8427370-8d01-5884-a98b-3fb441feffcd","slug":"rat-dlat-overexpression","display_name":"Dlat overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"hadha-attributed-lysate-activity","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adenoviral/cell perturbation study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"hadha-k728r-construct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 102, \"text_sha256\": \"299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff\", \"xml_element_id\": \"Par46\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"3 per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eb8093ed-0674-58e1-b864-81834d21dc44","evidence_kind":"source_excerpt","locator":"Lines 160-175","start_line":160,"end_line":175,"excerpt":"In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Adenoviral/cell perturbation study\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods\nduration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H\nendpoint: hadha-attributed-lysate-activity\nexposure: hadha-k728r-construct\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 102, \"text_sha256\": \"299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff\", \"xml_element_id\": \"Par46\"}]\nplain_language: In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.\nsample_size: 3 per group","model_system":"Adenoviral/cell perturbation study","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}