Component
HADHA-attributed activity in lysate assay
Paper-assigned HADHA activity; lysate assay specificity and protocol ambiguities are retained in claim limitations.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What acts on it
In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.
Experimental context and source evidence
- access_level
- full_text_and_supplement_review
- compartment
- Mitochondria or cell lysate, depending on assay
- dose
- Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods
- duration
- Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified
- endpoint
- hadha-attributed-lysate-activity
- evidence_location
- Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H
- experimental_model
- Adenoviral/cell perturbation study
- exposure
- hadha-k728r-construct
- limitations
- Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed.
- organism
- Rattus norvegicus host cells; construct species may be unspecified
- plain_language
- In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not.
- primary_locator
- [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}]
- primary_references
- https://doi.org/10.1038/s41467-026-70703-w
- sample_size
- 3 per group
- tissue_or_cell_type
- Neonatal rat ventricular cardiomyocytes (NRVCMs)
DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 160–175
AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Adenoviral/cell perturbation study · source_derived_draft · unverified_draft
In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not. organism: Rattus norvegicus host cells; construct species may be unspecified tissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs) experimental_model: Adenoviral/cell perturbation study compartment: Mitochondria or cell lysate, depending on assay dose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods duration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Lysate NADH/acetoacetyl-CoA assay, Figure 7G/H endpoint: hadha-attributed-lysate-activity exposure: hadha-k728r-construct limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Not an in vivo mouse K728R rescue. Lysate specificity is not independently established. Methods report an internally inconsistent 10 mL addition to a 190 µL assay; no protocol correction is assumed. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 48, "text_sha256": "134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e", "xml_element_id": "Par20"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 50, "text_sha256": "ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}] plain_language: In Dlat-overexpressing rat cardiomyocytes, K728R preserved the HADHA-attributed activity readout whereas K411R did not. sample_size: 3 per group
Complete structured claim and evidenceSpermidine increased the HADHA-attributed activity readout in mouse cardiac lysates from treated animals.
Experimental context and source evidence
- access_level
- full_text_and_supplement_review
- compartment
- Cardiac tissue / mitochondria
- dose
- 30 mM in drinking water, as reported
- duration
- 10 weeks in the Results; broader two-hit methods specify 15 weeks
- endpoint
- hadha-attributed-lysate-activity
- evidence_location
- Figure 8A/B
- experimental_model
- Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated
- exposure
- spermidine
- limitations
- Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. This paper does not itself establish direct spermidine binding; that statement cites earlier work. Do not infer intake in mg/kg from water concentration. Lysate specificity/protocol units remain qualified.
- nutrient_topic
- Topical cross-reference only; no inheritance of another actor's effects. · Spermidine
- organism
- Mus musculus
- plain_language
- Spermidine increased the HADHA-attributed activity readout in mouse cardiac lysates from treated animals.
- primary_locator
- [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 54, "text_sha256": "ecfe210aa0338ec52935a354f2bcc46289d65981b7b09fd4299e4e4f2fac4254", "xml_element_id": "Par22"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 56, "text_sha256": "b4baf8321f41630eff2d66a4181f2907f674041d430ba7878fd278f33d4141cf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 72, "text_sha256": "2b1ada63fe6c48d4296bbfb14a97940ac4a866b1d5171b0923851ec72f88ab15", "xml_element_id": "Par31"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}]
- primary_references
- https://doi.org/10.1038/s41467-026-70703-w
- route
- Drinking water
- sample_size
- 4 per group
- tissue_or_cell_type
- Cardiac tissue and cardiomyocytes
DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence · lines 641–657
AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation. · supports · Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated · source_derived_draft · unverified_draft
Spermidine increased the HADHA-attributed activity readout in mouse cardiac lysates from treated animals. organism: Mus musculus tissue_or_cell_type: Cardiac tissue and cardiomyocytes experimental_model: Mouse two-hit HFpEF model: 60% fat-calorie diet plus L-NAME 0.5 g/L drinking water; additional intervention as stated compartment: Cardiac tissue / mitochondria dose: 30 mM in drinking water, as reported duration: 10 weeks in the Results; broader two-hit methods specify 15 weeks route: Drinking water primary_references: https://doi.org/10.1038/s41467-026-70703-w access_level: full_text_and_supplement_review evidence_location: Figure 8A/B endpoint: hadha-attributed-lysate-activity exposure: spermidine limitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. This paper does not itself establish direct spermidine binding; that statement cites earlier work. Do not infer intake in mg/kg from water concentration. Lysate specificity/protocol units remain qualified. primary_locator: [{"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 54, "text_sha256": "ecfe210aa0338ec52935a354f2bcc46289d65981b7b09fd4299e4e4f2fac4254", "xml_element_id": "Par22"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 56, "text_sha256": "b4baf8321f41630eff2d66a4181f2907f674041d430ba7878fd278f33d4141cf", "xml_element_id": null}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 72, "text_sha256": "2b1ada63fe6c48d4296bbfb14a97940ac4a866b1d5171b0923851ec72f88ab15", "xml_element_id": "Par31"}, {"cache": "artifacts/dlat-curation/heart-blocks.json", "block_index": 102, "text_sha256": "299ae9af6e500af03d85d3cc327c60cb04e94f0618b6a9910780ac928e42faff", "xml_element_id": "Par46"}] plain_language: Spermidine increased the HADHA-attributed activity readout in mouse cardiac lysates from treated animals. sample_size: 4 per group
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.