{"id":"b4f0fe01-2d77-5e0c-89d5-6d0e926351ed","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:k728r-purified-control","predicate":"resists_induced_acetylation","statement":"Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e955de90-5fe7-5dd8-a3a0-82e57f09967e","mechanism_event_label":"k728r purified control","subject":{"id":"d693cf50-c5e0-5600-ae73-d67f0f8157e6","slug":"hadha-k728r-construct","display_name":"HADHA K728R experimental construct","entity_type_key":"protein_state"},"object":{"id":"f47c958c-2a43-5abc-8842-31700b1efa4c","slug":"hadha-construct-lysine-acetylation","display_name":"Acetyl-lysine signal on experimental HADHA constructs","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e955de90-5fe7-5dd8-a3a0-82e57f09967e","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:k728r-purified-control","event_type":"resists_induced_acetylation","label":"k728r purified control","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d693cf50-c5e0-5600-ae73-d67f0f8157e6","slug":"hadha-k728r-construct","display_name":"HADHA K728R experimental construct","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f47c958c-2a43-5abc-8842-31700b1efa4c","slug":"hadha-construct-lysine-acetylation","display_name":"Acetyl-lysine signal on experimental HADHA constructs","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80e2b56a-67d4-54a2-a847-2f55159ce64d","slug":"mouse-dlat","display_name":"Mouse dihydrolipoyl acetyltransferase / Dlat","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"acetyl donor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"In vitro reaction, not an intact-cell compartment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Acetyl-CoA present/absent controls; concentrations and incubation details incompletely specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Not specified in the reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"hadha-construct-lysine-acetylation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Figure 7E","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant-protein acetylation assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"hadha-k728r-construct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A construct-dependent result, not direct residue-specific occupancy quantification or a mouse-heart mutant experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Recombinant mouse Dlat; HADHA construct sequence species unresolved in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified-protein reaction","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"713cb2ea-6584-5963-8133-a34abb5a6ff2","evidence_kind":"source_excerpt","locator":"Lines 142-157","start_line":142,"end_line":157,"excerpt":"Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct.\norganism: Recombinant mouse Dlat; HADHA construct sequence species unresolved in reviewed methods\ntissue_or_cell_type: Purified-protein reaction\nexperimental_model: Recombinant-protein acetylation assay\ncompartment: In vitro reaction, not an intact-cell compartment\ndose: Acetyl-CoA present/absent controls; concentrations and incubation details incompletely specified\nduration: Not specified in the reviewed methods\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Figure 7E\nendpoint: hadha-construct-lysine-acetylation\nexposure: hadha-k728r-construct\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. A construct-dependent result, not direct residue-specific occupancy quantification or a mouse-heart mutant experiment.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 48, \"text_sha256\": \"134dcda50e7eafd608062a092c3cab71e5febe5935586f7d7d478988d6a3ea8e\", \"xml_element_id\": \"Par20\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 50, \"text_sha256\": \"ef3c37a398cc33c2cac08d4fc06ef96648c5fcda5ce3de26387b1afb609af2bf\", \"xml_element_id\": null}]\nplain_language: Dlat produced no detectable acetyl-lysine signal on the tested recombinant K728R HADHA construct.\nsample_size: 4 biological replicates","model_system":"Recombinant-protein acetylation assay","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}