{"id":"57edd876-60d9-5a39-8837-cfce3cc7891b","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-fao","predicate":"reduces","statement":"Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"252d9eec-c7c6-5512-9620-c9a8328dce88","mechanism_event_label":"rat dlat fao","subject":{"id":"e8427370-8d01-5884-a98b-3fb441feffcd","slug":"rat-dlat-overexpression","display_name":"Dlat overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"object":{"id":"6314b700-44b5-5993-aa8f-a90e13a25f02","slug":"rat-cardiac-fao","display_name":"Rat cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"252d9eec-c7c6-5512-9620-c9a8328dce88","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-fao","event_type":"reduces","label":"rat dlat fao","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e8427370-8d01-5884-a98b-3fb441feffcd","slug":"rat-dlat-overexpression","display_name":"Dlat overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6314b700-44b5-5993-aa8f-a90e13a25f02","slug":"rat-cardiac-fao","display_name":"Rat cardiac fatty-acid oxidation capacity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-cardiac-fao","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Seahorse palmitate oxidation stress test; Figure 3L–N","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adenoviral/cell perturbation study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"rat-dlat-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. OCR is a respiratory readout rather than a complete FAO flux balance. Etomoxir concentration was not specified in the reviewed methods.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 31, \"text_sha256\": \"74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0\", \"xml_element_id\": \"Par13\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 30, \"text_sha256\": \"c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 90, \"text_sha256\": \"9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700\", \"xml_element_id\": \"Par40\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 biological replicates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"66cf4818-e607-572b-8893-5b96f97a223b","evidence_kind":"source_excerpt","locator":"Lines 196-211","start_line":196,"end_line":211,"excerpt":"Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Adenoviral/cell perturbation study\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods\nduration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Seahorse palmitate oxidation stress test; Figure 3L–N\nendpoint: rat-cardiac-fao\nexposure: rat-dlat-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. OCR is a respiratory readout rather than a complete FAO flux balance. Etomoxir concentration was not specified in the reviewed methods.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 31, \"text_sha256\": \"74c923c72ae212e33ea648b25e8bc69db773a547139d5fa5b15971076fdeddd0\", \"xml_element_id\": \"Par13\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 30, \"text_sha256\": \"c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 90, \"text_sha256\": \"9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700\", \"xml_element_id\": \"Par40\"}]\nplain_language: Dlat overexpression lowered palmitate-supported oxygen consumption in neonatal rat cardiomyocytes.\nsample_size: 4 biological replicates","model_system":"Adenoviral/cell perturbation study","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}