{"id":"85fcc1f5-a5f9-5f61-9ce4-3e7859e15889","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-glycolysis","predicate":"increases","statement":"Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"3d38260c-f5f1-58d7-b33b-c55f07616f4e","mechanism_event_label":"rat dlat glycolysis","subject":{"id":"e8427370-8d01-5884-a98b-3fb441feffcd","slug":"rat-dlat-overexpression","display_name":"Dlat overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"object":{"id":"5caf75a8-5b06-58d6-adc7-2b855d307e6d","slug":"rat-cardiomyocyte-glycolytic-acidification","display_name":"Rat cardiomyocyte glycolysis-associated extracellular acidification","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"3d38260c-f5f1-58d7-b33b-c55f07616f4e","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-glycolysis","event_type":"increases","label":"rat dlat glycolysis","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e8427370-8d01-5884-a98b-3fb441feffcd","slug":"rat-dlat-overexpression","display_name":"Dlat overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5caf75a8-5b06-58d6-adc7-2b855d307e6d","slug":"rat-cardiomyocyte-glycolytic-acidification","display_name":"Rat cardiomyocyte glycolysis-associated extracellular acidification","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"rat-cardiomyocyte-glycolytic-acidification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Seahorse ECAR; Figure 3P–R","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adenoviral/cell perturbation study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"rat-dlat-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. ECAR is an assay readout, not a direct measure of PDH flux. The molecular cause remains unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 30, \"text_sha256\": \"c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 32, \"text_sha256\": \"ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731\", \"xml_element_id\": \"Par14\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 90, \"text_sha256\": \"9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700\", \"xml_element_id\": \"Par40\"}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d2447461-71b2-50cf-a744-53120e9bd871","evidence_kind":"source_excerpt","locator":"Lines 250-264","start_line":250,"end_line":264,"excerpt":"Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Adenoviral/cell perturbation study\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods\nduration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: Seahorse ECAR; Figure 3P–R\nendpoint: rat-cardiomyocyte-glycolytic-acidification\nexposure: rat-dlat-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. ECAR is an assay readout, not a direct measure of PDH flux. The molecular cause remains unresolved.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 30, \"text_sha256\": \"c2e9b88e22dc6b77f227fe9d4192d471b12452fe1e59280543fcc219fed2414f\", \"xml_element_id\": null}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 32, \"text_sha256\": \"ea1ec18a5210aa68822ce1416ae850de5ce871e100938a13a2c1dcc49c444731\", \"xml_element_id\": \"Par14\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 90, \"text_sha256\": \"9acf1ca568eece887ec72ad83ba8e593471afa5810e005762d0d3cbcfd80e700\", \"xml_element_id\": \"Par40\"}]\nplain_language: Dlat-overexpressing rat cardiomyocytes showed greater glycolysis-associated extracellular acidification.","model_system":"Adenoviral/cell perturbation study","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. 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