{"id":"6cc624f5-ac03-51bc-ba4f-3e2b0f4f3d5b","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-isotope-flux","predicate":"alters_tracer_products","statement":"Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"c6828461-5e65-57f7-94db-ccbf9278a7c8","mechanism_event_label":"rat dlat isotope flux","subject":{"id":"e8427370-8d01-5884-a98b-3fb441feffcd","slug":"rat-dlat-overexpression","display_name":"Dlat overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"object":{"id":"73cef62a-fc2e-5072-9fff-402e58d165fa","slug":"fatty-acid-oxidation","display_name":"Cellular fatty acid oxidation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c6828461-5e65-57f7-94db-ccbf9278a7c8","stable_key":"c156003d-8cdf-5259-8561-9803795ecc82:rat-dlat-isotope-flux","event_type":"alters_tracer_products","label":"rat dlat isotope flux","description":"Model-specific finding; read linked claim context and source-access tier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e8427370-8d01-5884-a98b-3fb441feffcd","slug":"rat-dlat-overexpression","display_name":"Dlat overexpression in neonatal rat ventricular cardiomyocytes","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"73cef62a-fc2e-5072-9fff-402e58d165fa","slug":"fatty-acid-oxidation","display_name":"Cellular fatty acid oxidation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"measured labeled product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"3acd8a9d-0b7e-5892-b35f-5ecbd005c352","slug":"rat-hadha","display_name":"Rat mitochondrial trifunctional protein alpha subunit / Hadha","entity_type_key":"protein"},"role":"candidate mediator","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"access_level","value_text":"full_text_and_supplement_review","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"compartment","value_text":"Mitochondria or cell lysate, depending on assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"endpoint","value_text":"fatty-acid-oxidation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"13C-palmitate tracing; Figure 6L","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Adenoviral/cell perturbation study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"rat-dlat-overexpression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Reported metabolite-labeling pattern supports impaired breakdown; it does not itself establish every kinetic step or isolate HADHA from other Dlat effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Rattus norvegicus host cells; construct species may be unspecified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"[{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1038/s41467-026-70703-w","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"sample_size","value_text":"4 per group","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neonatal rat ventricular cardiomyocytes (NRVCMs)","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f05a2919-8286-5003-ae36-b53ebcfe3367","evidence_kind":"source_excerpt","locator":"Lines 214-229","start_line":214,"end_line":229,"excerpt":"Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA.\norganism: Rattus norvegicus host cells; construct species may be unspecified\ntissue_or_cell_type: Neonatal rat ventricular cardiomyocytes (NRVCMs)\nexperimental_model: Adenoviral/cell perturbation study\ncompartment: Mitochondria or cell lysate, depending on assay\ndose: Adenovirus MOI 50:1 where applicable; compound concentrations not specified in reviewed methods\nduration: Adenovirus incubation 6–8 h; subsequent endpoint timing not consistently specified\nprimary_references: https://doi.org/10.1038/s41467-026-70703-w\naccess_level: full_text_and_supplement_review\nevidence_location: 13C-palmitate tracing; Figure 6L\nendpoint: fatty-acid-oxidation\nexposure: rat-dlat-overexpression\nlimitations: Single primary study; not independently replicated here. Cardiac and recombinant findings do not establish pulmonary endothelial, viral-sepsis or ARDS effects. Reported metabolite-labeling pattern supports impaired breakdown; it does not itself establish every kinetic step or isolate HADHA from other Dlat effects.\nprimary_locator: [{\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 44, \"text_sha256\": \"e0a92f47bc76c894816706ffb5158242c968928ac61e4561262c370035eda621\", \"xml_element_id\": \"Par19\"}, {\"cache\": \"artifacts/dlat-curation/heart-blocks.json\", \"block_index\": 46, \"text_sha256\": \"d56a90702bdf004de1efc2e43103b1b771e15b38288dc01f3741676782b1f5ff\", \"xml_element_id\": null}]\nplain_language: Dlat-overexpressing rat cardiomyocytes retained more labeled palmitoyl-CoA and showed less label in shorter acyl-CoAs and acetyl-CoA.\nsample_size: 4 per group","model_system":"Adenoviral/cell perturbation study","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Quotation is the authored curation, not original publisher wording. Exact primary locators and access limits retained in contexts.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ee72956d-4fd4-58d3-9a94-e173bb1e3282","stable_key":"import-c156003d-8cdf-5259-8561-9803795ecc82","title":"DLAT: cardiac fatty-acid oxidation and mitochondrial glutathione evidence","document_type":"imported_text","citation_label":"AI-assisted two-paper curation, 2026-09-20. Cardiac full-text/supplement review; CRC abstract only. No pulmonary endothelial validation.","file_path":"","sha256":"159e5472c8b1d104625f9c648dc9ee24dcd5fe102109fd960bba50e9ba9de954","revision_id":"5ca6b1f1-fd75-5f8d-ba2b-0215fbf387c7","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}