Component

Human TRPA1 ion channel

Human TRPA1 ion channel. Species, exposure and limitations are retained in each linked claim.

9 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Purified moringin acted as a potent agonist of human TRPA1 in vitro.

    Moringin / MIC-1 → Human TRPA1 ion channel source_derived_draftungraded
    Experimental context and source evidence
    dose
    Purified moringin concentration series
    duration
    Acute
    evidence_access
    Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
    evidence_scope
    literature_reviewed; model-specific source-derived curation
    experimental_model
    Human TRPA1-expressing in-vitro channel assay
    limitations
    Channel activation in an expression assay does not establish analgesia, pain, dose or tissue exposure in humans.
    nutrient_topic
    Moringa oleifera chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings. · Moringa oleifera
    organism
    Human TRPA1-expressing in-vitro channel assay
    plain_language
    Purified moringin acted as a potent agonist of human TRPA1 in vitro.
    primary_references
    Moringin, A Stable Isothiocyanate from Moringa oleifera, Activates the Somatosensory and Pain Receptor TRPA1 Channel In Vitro. (2020). https://pubmed.ncbi.nlm.nih.gov/32098328/ DOI: 10.3390/molecules25040976
    route
    In vitro
    tissue
    Somatosensory ion-channel activation

    Moringa oleifera: mechanism of action and interactions (2026-09-20) · lines 46–55

    Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text. · supports · Human TRPA1-expressing in-vitro channel assay · source_derived_draft · unverified_draft

    ## moringa-moringin-trpa1 Purified moringin acted as a potent agonist of human TRPA1 in vitro. Model/species: Human TRPA1-expressing in-vitro channel assay Tissue/system: Somatosensory ion-channel activation Exposure: Purified moringin concentration series Route: In vitro Duration: Acute Limits: Channel activation in an expression assay does not establish analgesia, pain, dose or tissue exposure in humans. Primary reference: Moringin, A Stable Isothiocyanate from Moringa oleifera, Activates the Somatosensory and Pain Receptor TRPA1 Channel In Vitro. (2020). https://pubmed.ncbi.nlm.nih.gov/32098328/ DOI: 10.3390/molecules25040976 Access: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
    Complete structured claim and evidence

  2. Cinnamaldehyde activated human TRPA1, with calcium-fluorescence EC50 approximately 11 micromolar in the expression assay.

    trans-Cinnamaldehyde / cinnamal → Human TRPA1 ion channel source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"}
    experimental_model
    Human TRPA1 expression, calcium fluorescence and patch clamp
    exposure
    Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct
    limitations
    Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect.
    nutrient_topic
    Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. · Ceylon cinnamon / Cinnamomum verum bark preparations
    organism
    Human TRPA1 in HEK293 cells
    plain_language
    Cinnamaldehyde can open a cellular sensor channel that lets a calcium signal develop.
    primary_references
    [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
    tissue_or_cell_type
    Engineered cultured cells

    Ceylon cinnamon: metabolism, signaling and nutrient connections (2026-09-17) · lines 337–348

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human TRPA1 expression, calcium fluorescence and patch clamp · source_derived_draft · unverified_draft

    ### ceylon-human-trpa1 Cinnamaldehyde activated human TRPA1, with calcium-fluorescence EC50 approximately 11 micromolar in the expression assay. Condition category: normal nutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. plain_language: Cinnamaldehyde can open a cellular sensor channel that lets a calcium signal develop. organism: Human TRPA1 in HEK293 cells tissue_or_cell_type: Engineered cultured cells experimental_model: Human TRPA1 expression, calcium fluorescence and patch clamp limitations: Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect. exposure: Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct evidence_span: {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"} [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
    Complete structured claim and evidence

  3. Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.

    Capsaicin → Human TRPA1 ion channel source_derived_draftungraded
    Experimental context and source evidence
    dose
    Capsaicin followed by mustard oil/TRPA1 stimulation
    duration
    Acute sequential exposure
    evidence_access
    Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
    evidence_scope
    literature_reviewed; model-specific source-derived curation
    experimental_model
    Sensory neurons and heterologous channel expression
    limitations
    The mechanism differed from mustard-oil homologous desensitization and was not regulated by calcineurin in this study.
    nutrient_topic
    Capsaicin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings. · Capsaicin
    organism
    Sensory neurons and heterologous channel expression
    plain_language
    Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.
    primary_references
    Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization. (2007). https://pubmed.ncbi.nlm.nih.gov/17584831/ DOI: 10.1113/jphysiol.2007.133231
    route
    In vitro
    tissue
    TRPA1 responses after TRPV1 agonism

    Capsaicin: mechanism of action and interactions (2026-09-20) · lines 55–64

    Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text. · supports · Sensory neurons and heterologous channel expression · source_derived_draft · unverified_draft

    ## capsaicin-trpa1-cross-desensitization Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion. Model/species: Sensory neurons and heterologous channel expression Tissue/system: TRPA1 responses after TRPV1 agonism Exposure: Capsaicin followed by mustard oil/TRPA1 stimulation Route: In vitro Duration: Acute sequential exposure Limits: The mechanism differed from mustard-oil homologous desensitization and was not regulated by calcineurin in this study. Primary reference: Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization. (2007). https://pubmed.ncbi.nlm.nih.gov/17584831/ DOI: 10.1113/jphysiol.2007.133231 Access: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
    Complete structured claim and evidence

  4. Gamma-nonalactone shifted the AITC response rightward without reducing its maximum.

    Gamma-nonalactone → Human TRPA1 ion channel source_derived_draftungraded
    Experimental context and source evidence
    dose
    Gamma-nonalactone 2.56 mM with an AITC concentration series
    duration
    Acute calcium response
    evidence_access
    Primary full-text methods/results inspected; PubMed metadata where indexed.
    evidence_scope
    literature_reviewed; model-specific source-derived curation
    experimental_model
    Human TRPA1-expressing HEK293 cells
    limitations
    Authors interpret an agonist interaction; the retained maximum does not establish pure antagonism. Delta-dodecalactone antagonism must not be assigned to gamma-nonalactone.
    nutrient_topic
    Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings. · Gamma-nonalactone
    organism
    Human TRPA1-expressing HEK293 cells
    plain_language
    Gamma-nonalactone shifted the AITC response rightward without reducing its maximum.
    primary_references
    Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023
    route
    In vitro co-application
    tissue
    AITC concentration-response assay

    Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 59–68

    Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text. · supports · Human TRPA1-expressing HEK293 cells · source_derived_draft · unverified_draft

    ## gamma-nonalactone-aitc-curve Gamma-nonalactone shifted the AITC response rightward without reducing its maximum. Model/species: Human TRPA1-expressing HEK293 cells Tissue: AITC concentration-response assay Exposure: Gamma-nonalactone 2.56 mM with an AITC concentration series Route: In vitro co-application Duration: Acute calcium response Limits: Authors interpret an agonist interaction; the retained maximum does not establish pure antagonism. Delta-dodecalactone antagonism must not be assigned to gamma-nonalactone. Primary reference: Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023 Access: Primary full-text methods/results inspected; PubMed metadata where indexed.
    Complete structured claim and evidence

  5. Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.

    Gamma-nonalactone → Human TRPA1 ion channel source_derived_draftungraded
    Experimental context and source evidence
    dose
    Gamma-nonalactone 2 mM; six replicates; separate concentration-response series
    duration
    Acute fluorescence response; precise recording window not specified in accessed methods
    evidence_access
    Primary full-text methods/results inspected; PubMed metadata where indexed.
    evidence_scope
    literature_reviewed; model-specific source-derived curation
    experimental_model
    Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells
    limitations
    Millimolar assay exposure is not dietary tissue exposure or clinical analgesia. Above 5 mM assay artifacts prevented reliable lactone EC50 fitting. Human receptor species confirmed in methods.
    nutrient_topic
    Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings. · Gamma-nonalactone
    organism
    Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells
    plain_language
    Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.
    primary_references
    Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023
    route
    In vitro bath addition
    tissue
    Fluo8 NW calcium-flux assay

    Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 26–35

    Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text. · supports · Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells · source_derived_draft · unverified_draft

    ## gamma-nonalactone-trpa1-activation Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells. Model/species: Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells Tissue: Fluo8 NW calcium-flux assay Exposure: Gamma-nonalactone 2 mM; six replicates; separate concentration-response series Route: In vitro bath addition Duration: Acute fluorescence response; precise recording window not specified in accessed methods Limits: Millimolar assay exposure is not dietary tissue exposure or clinical analgesia. Above 5 mM assay artifacts prevented reliable lactone EC50 fitting. Human receptor species confirmed in methods. Primary reference: Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023 Access: Primary full-text methods/results inspected; PubMed metadata where indexed.
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. In the same in-vitro comparison, moringin did not activate or only weakly activated TRPV1, TRPV2, TRPV3, TRPV4 and TRPM8 relative to its TRPA1 activity.

    Moringin / MIC-1 → Human TRPV1-TRPV4 and TRPM8 channel panel source_derived_draftungraded
    Experimental context and source evidence
    dose
    Purified moringin
    duration
    Acute
    evidence_access
    Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
    evidence_scope
    literature_reviewed; model-specific source-derived curation
    experimental_model
    Human TRP-channel in-vitro assays
    limitations
    This selectivity result is assay-specific and does not exclude indirect effects on sensory signaling in vivo.
    nutrient_topic
    Moringa oleifera chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings. · Moringa oleifera
    organism
    Human TRP-channel in-vitro assays
    plain_language
    In the same in-vitro comparison, moringin did not activate or only weakly activated TRPV1, TRPV2, TRPV3, TRPV4 and TRPM8 relative to its TRPA1 activity.
    primary_references
    Moringin, A Stable Isothiocyanate from Moringa oleifera, Activates the Somatosensory and Pain Receptor TRPA1 Channel In Vitro. (2020). https://pubmed.ncbi.nlm.nih.gov/32098328/ DOI: 10.3390/molecules25040976
    route
    In vitro
    tissue
    Channel selectivity panel

    Moringa oleifera: mechanism of action and interactions (2026-09-20) · lines 57–66

    Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text. · supports · Human TRP-channel in-vitro assays · source_derived_draft · unverified_draft

    ## moringa-moringin-trp-selectivity In the same in-vitro comparison, moringin did not activate or only weakly activated TRPV1, TRPV2, TRPV3, TRPV4 and TRPM8 relative to its TRPA1 activity. Model/species: Human TRP-channel in-vitro assays Tissue/system: Channel selectivity panel Exposure: Purified moringin Route: In vitro Duration: Acute Limits: This selectivity result is assay-specific and does not exclude indirect effects on sensory signaling in vivo. Primary reference: Moringin, A Stable Isothiocyanate from Moringa oleifera, Activates the Somatosensory and Pain Receptor TRPA1 Channel In Vitro. (2020). https://pubmed.ncbi.nlm.nih.gov/32098328/ DOI: 10.3390/molecules25040976 Access: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.
    Complete structured claim and evidence

  2. The human TRPA1 C621S/C641S/C665S construct did not respond to 300 micromolar cinnamaldehyde.

    Human TRPA1 C621S/C641S/C665S construct → trans-Cinnamaldehyde / cinnamal source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"}
    experimental_model
    Human TRPA1 expression, calcium fluorescence and patch clamp
    exposure
    Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct
    limitations
    Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect.
    nutrient_topic
    Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. · Ceylon cinnamon / Cinnamomum verum bark preparations
    organism
    Human TRPA1 in HEK293 cells
    plain_language
    Changing three reactive amino acids removed this chemical response.
    primary_references
    [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
    tissue_or_cell_type
    Engineered cultured cells
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Ceylon cinnamon: metabolism, signaling and nutrient connections (2026-09-17) · lines 350–361

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human TRPA1 expression, calcium fluorescence and patch clamp · source_derived_draft · unverified_draft

    ### ceylon-trpa1-mutant The human TRPA1 C621S/C641S/C665S construct did not respond to 300 micromolar cinnamaldehyde. Condition category: machinery_impairment nutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. plain_language: Changing three reactive amino acids removed this chemical response. organism: Human TRPA1 in HEK293 cells tissue_or_cell_type: Engineered cultured cells experimental_model: Human TRPA1 expression, calcium fluorescence and patch clamp limitations: Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect. exposure: Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct evidence_span: {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"} [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
    Complete structured claim and evidence

  3. A-967079 inhibited the gamma-nonalactone-evoked TRPA1 calcium response.

    A-967079 → Calcium response of human-TRPA1-expressing HEK293 cells source_derived_draftungraded
    Experimental context and source evidence
    dose
    Gamma-nonalactone 2 mM; capsazepine 1 micromolar or A-967079 1 micromolar
    duration
    Acute fluorescence assay
    evidence_access
    Primary full-text methods/results inspected; PubMed metadata where indexed.
    evidence_scope
    literature_reviewed; model-specific source-derived curation
    experimental_model
    Human TRPV1 or TRPA1 expressed in HEK293 cells
    limitations
    Pharmacological specificity controls support channel involvement; they do not establish a binding pocket or oral therapeutic exposure.
    nutrient_topic
    Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings. · Gamma-nonalactone
    organism
    Human TRPV1 or TRPA1 expressed in HEK293 cells
    plain_language
    A-967079 inhibited the gamma-nonalactone-evoked TRPA1 calcium response.
    primary_references
    Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023
    route
    In vitro co-exposure
    tissue
    Receptor antagonist controls

    Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 81–90

    Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text. · supports · Human TRPV1 or TRPA1 expressed in HEK293 cells · source_derived_draft · unverified_draft

    ## gamma-nonalactone-a967079-control A-967079 inhibited the gamma-nonalactone-evoked TRPA1 calcium response. Model/species: Human TRPV1 or TRPA1 expressed in HEK293 cells Tissue: Receptor antagonist controls Exposure: Gamma-nonalactone 2 mM; capsazepine 1 micromolar or A-967079 1 micromolar Route: In vitro co-exposure Duration: Acute fluorescence assay Limits: Pharmacological specificity controls support channel involvement; they do not establish a binding pocket or oral therapeutic exposure. Primary reference: Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023 Access: Primary full-text methods/results inspected; PubMed metadata where indexed.
    Complete structured claim and evidence

  4. EGTA treatment suppressed the gamma-nonalactone-evoked TRPA1 calcium signal.

    EGTA → Calcium response of human-TRPA1-expressing HEK293 cells source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    dose
    Gamma-nonalactone 2 mM and EGTA 5 mM
    duration
    Acute fluorescence assay
    evidence_access
    Primary full-text methods/results inspected; PubMed metadata where indexed.
    evidence_scope
    literature_reviewed; model-specific source-derived curation
    experimental_model
    Human TRPV1 or TRPA1 expressed in HEK293 cells
    limitations
    Experimental extracellular calcium removal is not dietary calcium deficiency. A calcium-flux readout cannot establish every downstream cellular consequence.
    nutrient_topic
    Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings. · Gamma-nonalactone
    organism
    Human TRPV1 or TRPA1 expressed in HEK293 cells
    plain_language
    EGTA treatment suppressed the gamma-nonalactone-evoked TRPA1 calcium signal.
    primary_references
    Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023
    route
    In vitro calcium chelation
    tissue
    Extracellular calcium-chelation control
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 92–101

    Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text. · supports · Human TRPV1 or TRPA1 expressed in HEK293 cells · source_derived_draft · unverified_draft

    ## gamma-nonalactone-egta-trpa1 EGTA treatment suppressed the gamma-nonalactone-evoked TRPA1 calcium signal. Model/species: Human TRPV1 or TRPA1 expressed in HEK293 cells Tissue: Extracellular calcium-chelation control Exposure: Gamma-nonalactone 2 mM and EGTA 5 mM Route: In vitro calcium chelation Duration: Acute fluorescence assay Limits: Experimental extracellular calcium removal is not dietary calcium deficiency. A calcium-flux readout cannot establish every downstream cellular consequence. Primary reference: Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023 Access: Primary full-text methods/results inspected; PubMed metadata where indexed.
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards