{"id":"1251b1e6-9f53-55a8-b52b-c507503a20b3","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-trpa1-activation","predicate":"activates","statement":"Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d2762f34-5199-5091-8349-534da3ba8da4","mechanism_event_label":"Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.","subject":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"object":{"id":"44366bfb-222d-5da6-911a-e853c3d7cdb7","slug":"human-trpa1","display_name":"Human TRPA1 ion channel","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d2762f34-5199-5091-8349-534da3ba8da4","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-trpa1-activation-event","event_type":"observed_relationship","label":"Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.","description":"Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44366bfb-222d-5da6-911a-e853c3d7cdb7","slug":"human-trpa1","display_name":"Human TRPA1 ion channel","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Gamma-nonalactone 2 mM; six replicates; separate concentration-response series","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute fluorescence response; precise recording window not specified in accessed methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results inspected; PubMed metadata where indexed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar assay exposure is not dietary tissue exposure or clinical analgesia. Above 5 mM assay artifacts prevented reliable lactone EC50 fitting. Human receptor species confirmed in methods.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Gamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro bath addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Fluo8 NW calcium-flux assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a84fa43e-b8e8-5dc2-a802-b6ccb928dd1c","evidence_kind":"source_excerpt","locator":"Lines 26-35","start_line":26,"end_line":35,"excerpt":"## gamma-nonalactone-trpa1-activation\nGamma-nonalactone evoked a calcium response in human-TRPA1-expressing HEK293 cells.\nModel/species: Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells\nTissue: Fluo8 NW calcium-flux assay\nExposure: Gamma-nonalactone 2 mM; six replicates; separate concentration-response series\nRoute: In vitro bath addition\nDuration: Acute fluorescence response; precise recording window not specified in accessed methods\nLimits: Millimolar assay exposure is not dietary tissue exposure or clinical analgesia. Above 5 mM assay artifacts prevented reliable lactone EC50 fitting. Human receptor species confirmed in methods.\nPrimary reference: Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023\nAccess: Primary full-text methods/results inspected; PubMed metadata where indexed.","model_system":"Human TRPV1 or TRPA1 stably expressed in Flp-In HEK293 cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73777c54-95a4-5b07-9d14-d4dd31949fb1","stable_key":"import-8600a864-6653-52c7-9718-1362c5af4577","title":"Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. 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