Component
Human TRPA1 C621S/C641S/C665S construct
Human TRPA1 C621S/C641S/C665S construct. Species, exposure and limitations are retained in each linked claim.
2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
The human TRPA1 C621S/C641S/C665S construct did not respond to 300 micromolar cinnamaldehyde.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_span
- {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"}
- experimental_model
- Human TRPA1 expression, calcium fluorescence and patch clamp
- exposure
- Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct
- limitations
- Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect.
- nutrient_topic
- Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. · Ceylon cinnamon / Cinnamomum verum bark preparations
- organism
- Human TRPA1 in HEK293 cells
- plain_language
- Changing three reactive amino acids removed this chemical response.
- primary_references
- [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
- tissue_or_cell_type
- Engineered cultured cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Ceylon cinnamon: metabolism, signaling and nutrient connections (2026-09-17) · lines 350–361
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human TRPA1 expression, calcium fluorescence and patch clamp · source_derived_draft · unverified_draft
### ceylon-trpa1-mutant The human TRPA1 C621S/C641S/C665S construct did not respond to 300 micromolar cinnamaldehyde. Condition category: machinery_impairment nutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. plain_language: Changing three reactive amino acids removed this chemical response. organism: Human TRPA1 in HEK293 cells tissue_or_cell_type: Engineered cultured cells experimental_model: Human TRPA1 expression, calcium fluorescence and patch clamp limitations: Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect. exposure: Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct evidence_span: {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"} [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
Complete structured claim and evidence
What acts on it
Arachidonic acid at 100 micromolar retained about 40% of the wild-type response in the triple-cysteine TRPA1 mutant.
Experimental context and source evidence
- availability_state
- machinery_impairment Imported condition classification; unverified.
- evidence_span
- {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"}
- experimental_model
- Human TRPA1 expression, calcium fluorescence and patch clamp
- exposure
- Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct
- limitations
- Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect.
- nutrient_topic
- Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. · Ceylon cinnamon / Cinnamomum verum bark preparations
- organism
- Human TRPA1 in HEK293 cells
- plain_language
- This fatty acid could still partly activate the altered channel, showing that channel triggers need not use the same mechanism.
- primary_references
- [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
- tissue_or_cell_type
- Engineered cultured cells
- trigger_kind
- machinery_impairment Imported condition classification; unverified.
Ceylon cinnamon: metabolism, signaling and nutrient connections (2026-09-17) · lines 363–374
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Human TRPA1 expression, calcium fluorescence and patch clamp · source_derived_draft · unverified_draft
### ceylon-trpa1-aa-mutant Arachidonic acid at 100 micromolar retained about 40% of the wild-type response in the triple-cysteine TRPA1 mutant. Condition category: machinery_impairment nutrient_topic: Ceylon cinnamon research collection; topical membership is not evidence of a direct dietary effect. plain_language: This fatty acid could still partly activate the altered channel, showing that channel triggers need not use the same mechanism. organism: Human TRPA1 in HEK293 cells tissue_or_cell_type: Engineered cultured cells experimental_model: Human TRPA1 expression, calcium fluorescence and patch clamp limitations: Channel expression and high cell-assay concentrations do not establish dietary exposure or a human calcium-deficiency effect. exposure: Cinnamaldehyde concentration response; 300 micromolar challenge; triple cysteine-to-serine construct evidence_span: {"source_cache": "artifacts/ceylon-research/24516781.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27", "start_char": 0, "end_char": 1685, "text_sha256": "5605569464035286614b12021fc60adbaf6c8a003a6b634d03fc5b544ce59d27"} [ceylon-p24516781] Ligand determinants of fatty acid activation of the pronociceptive ion channel TRPA1. (2014). https://pubmed.ncbi.nlm.nih.gov/24516781/ DOI: 10.7717/peerj.248
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.