{"id":"5464cde2-f6ea-54ee-a89d-2f234085a41d","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-trpa1-cross-desensitization","predicate":"cross_desensitizes","statement":"Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"bf5bfa9b-c285-53bc-927b-a7bc4d9aebac","mechanism_event_label":"Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.","subject":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"object":{"id":"44366bfb-222d-5da6-911a-e853c3d7cdb7","slug":"human-trpa1","display_name":"Human TRPA1 ion channel","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bf5bfa9b-c285-53bc-927b-a7bc4d9aebac","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-trpa1-cross-desensitization-event","event_type":"observed_relationship","label":"Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.","description":"Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"44366bfb-222d-5da6-911a-e853c3d7cdb7","slug":"human-trpa1","display_name":"Human TRPA1 ion channel","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5314e047-ba5d-52bb-8cfa-df41b6428824","slug":"pip2","display_name":"Phosphatidylinositol 4,5-bisphosphate / PIP2","entity_type_key":"lipid"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Capsaicin followed by mustard oil/TRPA1 stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute sequential exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Sensory neurons and heterologous channel expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The mechanism differed from mustard-oil homologous desensitization and was not regulated by calcineurin in this study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Capsaicin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Sensory neurons and heterologous channel expression","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Capsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization. (2007). https://pubmed.ncbi.nlm.nih.gov/17584831/ DOI: 10.1113/jphysiol.2007.133231","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"TRPA1 responses after TRPV1 agonism","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e5b85e15-61f0-5fb0-8790-029a29c352bb","evidence_kind":"source_excerpt","locator":"Lines 55-64","start_line":55,"end_line":64,"excerpt":"## capsaicin-trpa1-cross-desensitization\nCapsaicin cross-desensitized TRPA1 through a calcium-dependent route associated with PLC activation and PIP2 depletion.\nModel/species: Sensory neurons and heterologous channel expression\nTissue/system: TRPA1 responses after TRPV1 agonism\nExposure: Capsaicin followed by mustard oil/TRPA1 stimulation\nRoute: In vitro\nDuration: Acute sequential exposure\nLimits: The mechanism differed from mustard-oil homologous desensitization and was not regulated by calcineurin in this study.\nPrimary reference: Transient receptor potential TRPA1 channel desensitization in sensory neurons is agonist dependent and regulated by TRPV1-directed internalization. (2007). https://pubmed.ncbi.nlm.nih.gov/17584831/ DOI: 10.1113/jphysiol.2007.133231\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"Sensory neurons and heterologous channel expression","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"883c8afb-af48-5561-ae3b-00d4f3c26752","stable_key":"import-49fecf36-4958-5f15-8c78-9a68cba50763","title":"Capsaicin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"ea5c8dc49ffdfcec3f5542fed8be406c4af37873b76416910f88a743b733466e","revision_id":"79e49bef-520e-5503-ad2e-7de89ad236cf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}