Caffeic acid

evidence_span

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experimental_model

Randomized-order crossover in seven ileostomy volunteers

exposure

2.8 mmol chlorogenic acid or caffeic acid on separate days; 24-hour collections

limitations

Disappearance from ileal effluent is not intact-parent systemic bioavailability. Participants lacked normal colonic processing; no therapeutic outcome was tested.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human

plain_language

The free acid behaved differently from its quinic-acid ester.

primary_references

[chlorogenic_acid-p11208940] Chlorogenic acid and caffeic acid are absorbed in humans. (2001). https://pubmed.ncbi.nlm.nih.gov/11208940/ DOI: 10.1093/jn/131.1.66

tissue_or_cell_type

Small intestine; ileal effluent and urine

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 152–163

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Randomized-order crossover in seven ileostomy volunteers · source_derived_draft · unverified_draft

### chlorogenic_acid-caffeic-disappearance Caffeic-acid disappearance was 95 +/- 4% in the same crossover design. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: The free acid behaved differently from its quinic-acid ester. organism: Human tissue_or_cell_type: Small intestine; ileal effluent and urine experimental_model: Randomized-order crossover in seven ileostomy volunteers limitations: Disappearance from ileal effluent is not intact-parent systemic bioavailability. Participants lacked normal colonic processing; no therapeutic outcome was tested. exposure: 2.8 mmol chlorogenic acid or caffeic acid on separate days; 24-hour collections evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/11208940.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "651ac6849ea1490afeee5c905352bda75c8701decbcdf68f1d22dec05af06fd8", "start_char": 0, "end_char": 1299, "text_sha256": "651ac6849ea1490afeee5c905352bda75c8701decbcdf68f1d22dec05af06fd8"} [chlorogenic_acid-p11208940] Chlorogenic acid and caffeic acid are absorbed in humans. (2001). https://pubmed.ncbi.nlm.nih.gov/11208940/ DOI: 10.1093/jn/131.1.66

evidence_span

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experimental_model

Randomized-order crossover in seven ileostomy volunteers

exposure

2.8 mmol chlorogenic acid or caffeic acid on separate days; 24-hour collections

limitations

Disappearance from ileal effluent is not intact-parent systemic bioavailability. Participants lacked normal colonic processing; no therapeutic outcome was tested.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human

plain_language

The intact molecule differs from free caffeic acid.

primary_references

[chlorogenic_acid-p11208940] Chlorogenic acid and caffeic acid are absorbed in humans. (2001). https://pubmed.ncbi.nlm.nih.gov/11208940/ DOI: 10.1093/jn/131.1.66

tissue_or_cell_type

Small intestine; ileal effluent and urine

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 113–124

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Randomized-order crossover in seven ileostomy volunteers · source_derived_draft · unverified_draft

### chlorogenic_acid-caffeic-ester Chlorogenic acid contains a caffeic-acid moiety esterified to quinic acid. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: The intact molecule differs from free caffeic acid. organism: Human tissue_or_cell_type: Small intestine; ileal effluent and urine experimental_model: Randomized-order crossover in seven ileostomy volunteers limitations: Disappearance from ileal effluent is not intact-parent systemic bioavailability. Participants lacked normal colonic processing; no therapeutic outcome was tested. exposure: 2.8 mmol chlorogenic acid or caffeic acid on separate days; 24-hour collections evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/11208940.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "651ac6849ea1490afeee5c905352bda75c8701decbcdf68f1d22dec05af06fd8", "start_char": 0, "end_char": 1299, "text_sha256": "651ac6849ea1490afeee5c905352bda75c8701decbcdf68f1d22dec05af06fd8"} [chlorogenic_acid-p11208940] Chlorogenic acid and caffeic acid are absorbed in humans. (2001). https://pubmed.ncbi.nlm.nih.gov/11208940/ DOI: 10.1093/jn/131.1.66

evidence_span

{"source_cache": "artifacts/chlorogenic_acid-research/11412317.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6", "start_char": 0, "end_char": 1812, "text_sha256": "2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6"}

experimental_model

Culture and enzymatic characterization of human fecal isolates

exposure

Thirty-five isolates screened; six esterase-positive isolates identified

limitations

Activity was strain-specific and genes were not identified. The study does not establish that all members of a named species or commercial probiotics have this activity.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human-derived bacterial isolates

plain_language

Some gut bacteria can open the ester, changing the compounds available to the host.

primary_references

[chlorogenic_acid-p11412317] Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid. (2001). https://pubmed.ncbi.nlm.nih.gov/11412317/ DOI: 10.1046/j.1365-2672.2001.01316.x

tissue_or_cell_type

Anaerobic cultures; intracellular esterase activity

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 204–215

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Culture and enzymatic characterization of human fecal isolates · source_derived_draft · unverified_draft

### chlorogenic_acid-bacterial-hydrolysis Chlorogenic-acid hydrolysis was detected in selected E. coli, B. lactis and L. gasseri isolates; activity was mainly intracellular. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: Some gut bacteria can open the ester, changing the compounds available to the host. organism: Human-derived bacterial isolates tissue_or_cell_type: Anaerobic cultures; intracellular esterase activity experimental_model: Culture and enzymatic characterization of human fecal isolates limitations: Activity was strain-specific and genes were not identified. The study does not establish that all members of a named species or commercial probiotics have this activity. exposure: Thirty-five isolates screened; six esterase-positive isolates identified evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/11412317.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6", "start_char": 0, "end_char": 1812, "text_sha256": "2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6"} [chlorogenic_acid-p11412317] Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid. (2001). https://pubmed.ncbi.nlm.nih.gov/11412317/ DOI: 10.1046/j.1365-2672.2001.01316.x

evidence_span

{"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"}

experimental_model

Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments

exposure

CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar

limitations

These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract

plain_language

Metabolizing a catechol connects it to methyl-group chemistry.

primary_references

[chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

tissue_or_cell_type

Cell-free methyltransferase reactions and cultured cancer cells

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 776–787

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments · source_derived_draft · unverified_draft

### chlorogenic_acid-comt-cga COMT-mediated O-methylation of CGA contributed to the coupled-assay response. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: Metabolizing a catechol connects it to methyl-group chemistry. organism: Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract tissue_or_cell_type: Cell-free methyltransferase reactions and cultured cancer cells experimental_model: Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments limitations: These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1. exposure: CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"} [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

evidence_span

{"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"}

experimental_model

Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments

exposure

CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar

limitations

These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract

plain_language

Using a methyl donor leaves a product that can restrain other methyltransferases.

primary_references

[chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

tissue_or_cell_type

Cell-free methyltransferase reactions and cultured cancer cells

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 789–800

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments · source_derived_draft · unverified_draft

### chlorogenic_acid-comt-sah O-methylation of the dietary catechols increased SAH formation. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: Using a methyl donor leaves a product that can restrain other methyltransferases. organism: Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract tissue_or_cell_type: Cell-free methyltransferase reactions and cultured cancer cells experimental_model: Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments limitations: These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1. exposure: CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"} [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

evidence_span

{"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"}

experimental_model

Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments

exposure

CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar

limitations

These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract

plain_language

The concentration belongs to this coupled biochemical assay.

primary_references

[chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

tissue_or_cell_type

Cell-free methyltransferase reactions and cultured cancer cells

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 815–826

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments · source_derived_draft · unverified_draft

### chlorogenic_acid-dnmt-assay CGA inhibited human DNMT1-mediated methylation with an assay IC50 of 0.9 micromolar. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: The concentration belongs to this coupled biochemical assay. organism: Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract tissue_or_cell_type: Cell-free methyltransferase reactions and cultured cancer cells experimental_model: Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments limitations: These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1. exposure: CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"} [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

evidence_span

{"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"}

experimental_model

Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments

exposure

CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar

limitations

These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract

plain_language

One promoter in cancer cells does not describe genome-wide epigenetic health.

primary_references

[chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

tissue_or_cell_type

Cell-free methyltransferase reactions and cultured cancer cells

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 828–839

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments · source_derived_draft · unverified_draft

### chlorogenic_acid-rarb-methylation The cancer-cell experiments reported partial inhibition of RARB promoter methylation. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: One promoter in cancer cells does not describe genome-wide epigenetic health. organism: Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract tissue_or_cell_type: Cell-free methyltransferase reactions and cultured cancer cells experimental_model: Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments limitations: These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1. exposure: CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"} [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

evidence_span

{"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"}

experimental_model

Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments

exposure

CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar

limitations

These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1.

nutrient_topic

Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid

organism

Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract

plain_language

An indirect metabolite-mediated effect differs from a direct DNMT1 inhibitor.

primary_references

[chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206

tissue_or_cell_type

Cell-free methyltransferase reactions and cultured cancer cells

Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 802–813

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments · source_derived_draft · unverified_draft

### chlorogenic_acid-sah-dnmt Increased SAH largely explained the observed inhibition of DNA methylation. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: An indirect metabolite-mediated effect differs from a direct DNMT1 inhibitor. organism: Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract tissue_or_cell_type: Cell-free methyltransferase reactions and cultured cancer cells experimental_model: Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments limitations: These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1. exposure: CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"} [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206