{"id":"14629159-7db9-523a-a177-241e9ec31b14","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-esterase-hydrolysis","predicate":"reported_relationship","statement":"Fungal chlorogenate esterase hydrolyzed about 90% of extract RA in the reported in-vitro incubation.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"cf2666ca-75cc-5986-aa34-07bd195ffc4b","mechanism_event_label":"Fungal chlorogenate esterase hydrolyzed about 90% of extract RA in the reported in-vitro incubation.","subject":{"id":"ab422533-9a1a-522b-a0b9-b38fa4284d9f","slug":"aspergillus-japonicus-chlorogenate-esterase","display_name":"Aspergillus japonicus chlorogenate esterase","entity_type_key":"protein"},"object":{"id":"049cdeae-4cd8-5eac-b3c5-5a24a235a945","slug":"caffeic-acid","display_name":"Caffeic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"cf2666ca-75cc-5986-aa34-07bd195ffc4b","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-esterase-hydrolysis-event","event_type":"biochemical_relationship","label":"Fungal chlorogenate esterase hydrolyzed about 90% of extract RA in the reported in-vitro incubation.","description":"### ra-esterase-hydrolysis\n\nFungal chlorogenate esterase hydrolyzed about 90% of extract RA in the reported in-vitro incubation.\nThe cleavage connects the same caffeic-acid identity used in other chapters.\nModel and exposure: RA 5 mg/mL; enzyme 0.02 U/mg; pH 7, 40 °C, 2 h.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Hydrolysis of rosmarinic acid from rosemary extract with esterases and Lactobacillus johnsonii in vitro and in a gastrointestinal model. | 2009 | https://pubmed.ncbi.nlm.nih.gov/19658402/ | https://doi.org/10.1021/jf9014262 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ab422533-9a1a-522b-a0b9-b38fa4284d9f","slug":"aspergillus-japonicus-chlorogenate-esterase","display_name":"Aspergillus japonicus chlorogenate esterase","entity_type_key":"protein"},"role":"recorded factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"049cdeae-4cd8-5eac-b3c5-5a24a235a945","slug":"caffeic-acid","display_name":"Caffeic acid","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f6f74e9-1946-5bb9-b9ec-10d144aca1ea","slug":"3-4-dihydroxyphenyllactic-acid","display_name":"3,4-Dihydroxyphenyllactic acid / danshensu","entity_type_key":"small_molecule"},"role":"hydrolysis coproduct","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"RA 5 mg/mL; enzyme 0.02 U/mg; pH 7, 40 °C, 2 h.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Fungal chlorogenate esterase hydrolyzed about 90% of extract RA in the reported in-vitro incubation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Hydrolysis of rosmarinic acid from rosemary extract with esterases and Lactobacillus johnsonii in vitro and in a gastrointestinal model. | https://pubmed.ncbi.nlm.nih.gov/19658402/ | https://doi.org/10.1021/jf9014262","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-esterase-hydrolysis; The cleavage connects the same caffeic-acid identity used in other chapters.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6c04e073-b0a5-5a42-ab40-2afe00777699","evidence_kind":"source_excerpt","locator":"Lines 77-83","start_line":77,"end_line":83,"excerpt":"### ra-esterase-hydrolysis\n\nFungal chlorogenate esterase hydrolyzed about 90% of extract RA in the reported in-vitro incubation.\nThe cleavage connects the same caffeic-acid identity used in other chapters.\nModel and exposure: RA 5 mg/mL; enzyme 0.02 U/mg; pH 7, 40 °C, 2 h.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Hydrolysis of rosmarinic acid from rosemary extract with esterases and Lactobacillus johnsonii in vitro and in a gastrointestinal model. | 2009 | https://pubmed.ncbi.nlm.nih.gov/19658402/ | https://doi.org/10.1021/jf9014262 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"RA 5 mg/mL; enzyme 0.02 U/mg; pH 7, 40 °C, 2 h.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}