Component

Human DNA methyltransferase 1 / DNMT1

Human DNA methyltransferase 1 / DNMT1. Species, exposure and limitations are retained in each linked claim.

2 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. CGA inhibited human DNMT1-mediated methylation with an assay IC50 of 0.9 micromolar.

    Chlorogenic acid / 5-O-caffeoylquinic acid → Human DNA methyltransferase 1 / DNMT1 source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"}
    experimental_model
    Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments
    exposure
    CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar
    limitations
    These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1.
    nutrient_topic
    Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid
    organism
    Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract
    plain_language
    The concentration belongs to this coupled biochemical assay.
    primary_references
    [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206
    tissue_or_cell_type
    Cell-free methyltransferase reactions and cultured cancer cells

    Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 815–826

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments · source_derived_draft · unverified_draft

    ### chlorogenic_acid-dnmt-assay CGA inhibited human DNMT1-mediated methylation with an assay IC50 of 0.9 micromolar. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: The concentration belongs to this coupled biochemical assay. organism: Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract tissue_or_cell_type: Cell-free methyltransferase reactions and cultured cancer cells experimental_model: Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments limitations: These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1. exposure: CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"} [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206
    Complete structured claim and evidence

  2. Increased SAH largely explained the observed inhibition of DNA methylation.

    S-Adenosyl-L-homocysteine → Human DNA methyltransferase 1 / DNMT1 source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"}
    experimental_model
    Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments
    exposure
    CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar
    limitations
    These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1.
    nutrient_topic
    Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid
    organism
    Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract
    plain_language
    An indirect metabolite-mediated effect differs from a direct DNMT1 inhibitor.
    primary_references
    [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206
    tissue_or_cell_type
    Cell-free methyltransferase reactions and cultured cancer cells

    Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 802–813

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments · source_derived_draft · unverified_draft

    ### chlorogenic_acid-sah-dnmt Increased SAH largely explained the observed inhibition of DNA methylation. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: An indirect metabolite-mediated effect differs from a direct DNMT1 inhibitor. organism: Human DNMT1 and MCF-7/MDA-MB-231 cells; COMT preparation species unresolved in abstract tissue_or_cell_type: Cell-free methyltransferase reactions and cultured cancer cells experimental_model: Coupled catechol-methylation/DNA-methylation assays and breast-cancer cell experiments limitations: These concentrations and coupled-reaction conditions do not establish whole-body methyl-donor depletion, anticancer benefit or vitamin deficiency after food intake. COMT-mediated SAH generation is distinct from direct competitive binding to DNMT1. exposure: CGA DNMT1 IC50 0.9 micromolar in the coupled assay; compounds tested up to 20 micromolar evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/16081510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170", "start_char": 0, "end_char": 2008, "text_sha256": "e93fafd7946f8a4aea30ff3ee9ef05e303f9154eb947410fca8e63c6521a4170"} [chlorogenic_acid-p16081510] Inhibition of DNA methylation by caffeic acid and chlorogenic acid, two common catechol-containing coffee polyphenols. (2006). https://pubmed.ncbi.nlm.nih.gov/16081510/ DOI: 10.1093/carcin/bgi206
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards