{"id":"6e08fdf9-cce3-5739-abb0-00b450930450","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-bacterial-hydrolysis","predicate":"hydrolyze","statement":"Chlorogenic-acid hydrolysis was detected in selected E. coli, B. lactis and L. gasseri isolates; activity was mainly intracellular.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b9cf8343-dea6-569e-8ec2-497b7686e628","mechanism_event_label":"Some gut bacteria can open the ester, changing the compounds available to the host.","subject":{"id":"e9875eb7-8cb0-5a4b-97dd-23655faabc84","slug":"gut-bacterial-cinnamoyl-esterases","display_name":"Gut-isolate cinnamoyl esterase activities, genes unresolved","entity_type_key":"protein_family"},"object":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b9cf8343-dea6-569e-8ec2-497b7686e628","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-bacterial-hydrolysis-event","event_type":"biochemical_relationship","label":"Some gut bacteria can open the ester, changing the compounds available to the host.","description":"Chlorogenic-acid hydrolysis was detected in selected E. coli, B. lactis and L. gasseri isolates; activity was mainly intracellular.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"049cdeae-4cd8-5eac-b3c5-5a24a235a945","slug":"caffeic-acid","display_name":"Caffeic acid","entity_type_key":"small_molecule"},"role":"hydrolysis_product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9a4496d4-1aff-503e-95aa-46dd719a1dc5","slug":"quinic-acid","display_name":"Quinic acid","entity_type_key":"small_molecule"},"role":"hydrolysis_product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e9875eb7-8cb0-5a4b-97dd-23655faabc84","slug":"gut-bacterial-cinnamoyl-esterases","display_name":"Gut-isolate cinnamoyl esterase activities, genes unresolved","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chlorogenic_acid-research/11412317.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6\", \"start_char\": 0, \"end_char\": 1812, \"text_sha256\": \"2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Culture and enzymatic characterization of human fecal isolates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Thirty-five isolates screened; six esterase-positive isolates identified","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Activity was strain-specific and genes were not identified. The study does not establish that all members of a named species or commercial probiotics have this activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human-derived bacterial isolates","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Some gut bacteria can open the ester, changing the compounds available to the host.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chlorogenic_acid-p11412317] Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid. (2001). https://pubmed.ncbi.nlm.nih.gov/11412317/ DOI: 10.1046/j.1365-2672.2001.01316.x","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Anaerobic cultures; intracellular esterase activity","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c4ab896f-9f76-5ceb-844e-12e502de3757","evidence_kind":"source_excerpt","locator":"Lines 204-215","start_line":204,"end_line":215,"excerpt":"### chlorogenic_acid-bacterial-hydrolysis\nChlorogenic-acid hydrolysis was detected in selected E. coli, B. lactis and L. gasseri isolates; activity was mainly intracellular.\nCondition category: normal\nnutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Some gut bacteria can open the ester, changing the compounds available to the host.\norganism: Human-derived bacterial isolates\ntissue_or_cell_type: Anaerobic cultures; intracellular esterase activity\nexperimental_model: Culture and enzymatic characterization of human fecal isolates\nlimitations: Activity was strain-specific and genes were not identified. The study does not establish that all members of a named species or commercial probiotics have this activity.\nexposure: Thirty-five isolates screened; six esterase-positive isolates identified\nevidence_span: {\"source_cache\": \"artifacts/chlorogenic_acid-research/11412317.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6\", \"start_char\": 0, \"end_char\": 1812, \"text_sha256\": \"2c6244869c8a620b69559006cf5bd4a71d44839b0920a8b388eb933be1cfa9a6\"}\n[chlorogenic_acid-p11412317] Isolation and characterization of human colonic bacteria able to hydrolyse chlorogenic acid. 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