Component

Methanol

Context-specific entity; species, compartment and exposure are stated on each claim.

6 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Labeled methanol inhalation produced labeled blood formate in cynomolgus monkeys.

    Methanol → Formate source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary abstract
    experimental_model
    Four female monkeys; lung-only exposure for two hours at 10–900 ppm.
    limitations
    Not an aspartame feeding study; the route and systemic dose differ from digestion of a food sweetener.
    nutrient_topic
    Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Aspartame
    plain_language
    A traced precursor-product link connects two separate metabolite nodes.
    primary_references
    Pharmacokinetics of inhaled [14C]methanol and methanol-derived [14C]formate in normal and folate-deficient cynomolgus monkeys. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7940538/ · DOI 10.1006/taap.1994.1202

    Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20) · lines 354–360

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Four female monkeys; lung-only exposure for two hours at 10–900 ppm. · source_derived_draft · unverified_draft

    ## aspartame-methanol-formate-tracer A traced precursor-product link connects two separate metabolite nodes. Labeled methanol inhalation produced labeled blood formate in cynomolgus monkeys. Model: Four female monkeys; lung-only exposure for two hours at 10–900 ppm. Limitations: Not an aspartame feeding study; the route and systemic dose differ from digestion of a food sweetener. Evidence access: Primary abstract Pharmacokinetics of inhaled [14C]methanol and methanol-derived [14C]formate in normal and folate-deficient cynomolgus monkeys. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7940538/ · DOI 10.1006/taap.1994.1202
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. The intestinal study describes ester-bond hydrolysis of aspartame to Asp-Phe before further dipeptide digestion.

    Aspartame → Alpha-L-aspartyl-L-phenylalanine source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary abstract
    experimental_model
    Reaction background and human intestinal mucosal enzyme experiments.
    limitations
    This record does not assign the initial esterase to an unverified gene.
    nutrient_topic
    Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Aspartame
    plain_language
    Removing the methyl ester is separate from splitting the peptide.
    primary_references
    Intestinal hydrolysis of aspartylphenylalanine--the metabolic product of aspartame. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3743970/ · DOI 10.1016/0016-5085(86)90697-9

    Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20) · lines 18–24

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Reaction background and human intestinal mucosal enzyme experiments. · source_derived_draft · unverified_draft

    ## aspartame-ester-cleavage Removing the methyl ester is separate from splitting the peptide. The intestinal study describes ester-bond hydrolysis of aspartame to Asp-Phe before further dipeptide digestion. Model: Reaction background and human intestinal mucosal enzyme experiments. Limitations: This record does not assign the initial esterase to an unverified gene. Evidence access: Primary abstract Intestinal hydrolysis of aspartylphenylalanine--the metabolic product of aspartame. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3743970/ · DOI 10.1016/0016-5085(86)90697-9
    Complete structured claim and evidence

  2. Four cynomolgus monkeys had higher labeled blood formate after 900 ppm methanol inhalation when folate-deficient than before depletion, while tracer-derived concentrations remained below endogenous formate.

    Folate (vitamin B9) → Formate source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Two-hour lung-only labeled-methanol exposure; repeated after diet-induced folate deficiency.
    limitations
    This was not aspartame feeding, and does not show aspartame causes folate depletion.
    nutrient_topic
    Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Aspartame
    plain_language
    Downstream handling depends on nutrient state and exposure route.
    primary_references
    Pharmacokinetics of inhaled [14C]methanol and methanol-derived [14C]formate in normal and folate-deficient cynomolgus monkeys. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7940538/ · DOI 10.1006/taap.1994.1202
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20) · lines 154–160

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Two-hour lung-only labeled-methanol exposure; repeated after diet-induced folate deficiency. · source_derived_draft · unverified_draft

    ## aspartame-folate-methanol-context Downstream handling depends on nutrient state and exposure route. Four cynomolgus monkeys had higher labeled blood formate after 900 ppm methanol inhalation when folate-deficient than before depletion, while tracer-derived concentrations remained below endogenous formate. Model: Two-hour lung-only labeled-methanol exposure; repeated after diet-induced folate deficiency. Limitations: This was not aspartame feeding, and does not show aspartame causes folate depletion. Evidence access: Primary abstract Pharmacokinetics of inhaled [14C]methanol and methanol-derived [14C]formate in normal and folate-deficient cynomolgus monkeys. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7940538/ · DOI 10.1006/taap.1994.1202
    Complete structured claim and evidence

  3. Six adults receiving 200 mg/kg showed no significant increase in blood formate over baseline.

    Aspartame → Human blood formate after aspartame loading source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Highest-dose subset of the loading study.
    limitations
    Limited sample, duration and assay; no inference about all metabolic disorders.
    nutrient_topic
    Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Aspartame
    plain_language
    Methanol appearance did not imply measured formate accumulation.
    primary_references
    Blood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20) · lines 130–136

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Highest-dose subset of the loading study. · source_derived_draft · unverified_draft

    ## aspartame-formate-loading Methanol appearance did not imply measured formate accumulation. Six adults receiving 200 mg/kg showed no significant increase in blood formate over baseline. Model: Highest-dose subset of the loading study. Limitations: Limited sample, duration and assay; no inference about all metabolic disorders. Evidence access: Primary abstract Blood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979
    Complete structured claim and evidence

  4. Blood methanol was below the 0.4 mg/dL detection limit at 34 mg/kg; 100–200 mg/kg doses produced dose-related peaks of 1.27–2.58 mg/dL.

    Aspartame → Human blood methanol after aspartame loading source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    biomarker_context Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Thirty adults across dose cohorts.
    limitations
    These bolus doses and old detection limits do not define a universal toxicity threshold.
    nutrient_topic
    Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Aspartame
    plain_language
    A non-detect depends on the assay; large doses gave measurable exposure.
    primary_references
    Blood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979
    trigger_kind
    biomarker_context Imported condition classification; unverified.

    Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20) · lines 122–128

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Thirty adults across dose cohorts. · source_derived_draft · unverified_draft

    ## aspartame-methanol-dose A non-detect depends on the assay; large doses gave measurable exposure. Blood methanol was below the 0.4 mg/dL detection limit at 34 mg/kg; 100–200 mg/kg doses produced dose-related peaks of 1.27–2.58 mg/dL. Model: Thirty adults across dose cohorts. Limitations: These bolus doses and old detection limits do not define a universal toxicity threshold. Evidence access: Primary abstract Blood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979
    Complete structured claim and evidence

  5. Methanol and formate remained within reported normal limits; plasma aspartate did not significantly rise in the eight-serving experiment.

    Aspartame → Human blood methanol/formate during repeated aspartame ingestion source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary abstract
    experimental_model
    Six healthy young adults.
    limitations
    Normal range is protocol- and assay-dependent, not proof that no metabolism occurred.
    nutrient_topic
    Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Aspartame
    plain_language
    Products can be generated without a sustained high blood pool.
    primary_references
    Effect of repeated ingestion of aspartame-sweetened beverage on plasma amino acid, blood methanol, and blood formate concentrations in normal adults. · 1989 · https://pubmed.ncbi.nlm.nih.gov/2566887/ · DOI 10.1016/0026-0495(89)90125-x

    Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20) · lines 114–120

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Six healthy young adults. · source_derived_draft · unverified_draft

    ## aspartame-repeated-metabolites Products can be generated without a sustained high blood pool. Methanol and formate remained within reported normal limits; plasma aspartate did not significantly rise in the eight-serving experiment. Model: Six healthy young adults. Limitations: Normal range is protocol- and assay-dependent, not proof that no metabolism occurred. Evidence access: Primary abstract Effect of repeated ingestion of aspartame-sweetened beverage on plasma amino acid, blood methanol, and blood formate concentrations in normal adults. · 1989 · https://pubmed.ncbi.nlm.nih.gov/2566887/ · DOI 10.1016/0026-0495(89)90125-x
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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