{"id":"f9155cde-c599-5f64-b295-822450325c8a","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-formate-loading","predicate":"does_not_significantly_increase_tested","statement":"Six adults receiving 200 mg/kg showed no significant increase in blood formate over baseline.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f169f062-542f-50d3-a686-9be54a45f135","mechanism_event_label":"Methanol appearance did not imply measured formate accumulation.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"2ec19f43-3c34-5425-950a-c5284488be0b","slug":"human-aspartame-blood-formate","display_name":"Human blood formate after aspartame loading","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f169f062-542f-50d3-a686-9be54a45f135","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-formate-loading-event","event_type":"observed_relationship","label":"Methanol appearance did not imply measured formate accumulation.","description":"Six adults receiving 200 mg/kg showed no significant increase in blood formate over baseline.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2ec19f43-3c34-5425-950a-c5284488be0b","slug":"human-aspartame-blood-formate","display_name":"Human blood formate after aspartame loading","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"96bbf620-a0f1-506b-9f01-a4f1ea1224a9","slug":"methanol","display_name":"Methanol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Highest-dose subset of the loading study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited sample, duration and assay; no inference about all metabolic disorders.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Methanol appearance did not imply measured formate accumulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Blood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"28eafacf-cecb-5b6d-b9cb-595e59ec271c","evidence_kind":"source_excerpt","locator":"Lines 130-136","start_line":130,"end_line":136,"excerpt":"## aspartame-formate-loading\nMethanol appearance did not imply measured formate accumulation.\nSix adults receiving 200 mg/kg showed no significant increase in blood formate over baseline.\nModel: Highest-dose subset of the loading study.\nLimitations: Limited sample, duration and assay; no inference about all metabolic disorders.\nEvidence access: Primary abstract\nBlood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979","model_system":"Highest-dose subset of the loading study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"21cfc6808062c6ea1fb5d34128bcd005d31f0f6c0d63484794238db77d32ae97","revision_id":"1255dda8-df99-5d4b-a65b-4144c47d7d9b","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}