{"id":"7b7bde67-1719-58bc-a14e-19e0d907702e","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-methanol-dose","predicate":"increases_at_high_tested_doses","statement":"Blood methanol was below the 0.4 mg/dL detection limit at 34 mg/kg; 100–200 mg/kg doses produced dose-related peaks of 1.27–2.58 mg/dL.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f550087b-167e-505c-845b-8f8671ead768","mechanism_event_label":"A non-detect depends on the assay; large doses gave measurable exposure.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"dfc3ffa9-a7ab-5a3a-b6c8-549f53c32d20","slug":"human-aspartame-blood-methanol","display_name":"Human blood methanol after aspartame loading","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f550087b-167e-505c-845b-8f8671ead768","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-methanol-dose-event","event_type":"observed_relationship","label":"A non-detect depends on the assay; large doses gave measurable exposure.","description":"Blood methanol was below the 0.4 mg/dL detection limit at 34 mg/kg; 100–200 mg/kg doses produced dose-related peaks of 1.27–2.58 mg/dL.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dfc3ffa9-a7ab-5a3a-b6c8-549f53c32d20","slug":"human-aspartame-blood-methanol","display_name":"Human blood methanol after aspartame loading","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"96bbf620-a0f1-506b-9f01-a4f1ea1224a9","slug":"methanol","display_name":"Methanol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Thirty adults across dose cohorts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These bolus doses and old detection limits do not define a universal toxicity threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A non-detect depends on the assay; large doses gave measurable exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Blood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2687def4-c043-586c-bb4d-82c3cc3fe978","evidence_kind":"source_excerpt","locator":"Lines 122-128","start_line":122,"end_line":128,"excerpt":"## aspartame-methanol-dose\nA non-detect depends on the assay; large doses gave measurable exposure.\nBlood methanol was below the 0.4 mg/dL detection limit at 34 mg/kg; 100–200 mg/kg doses produced dose-related peaks of 1.27–2.58 mg/dL.\nModel: Thirty adults across dose cohorts.\nLimitations: These bolus doses and old detection limits do not define a universal toxicity threshold.\nEvidence access: Primary abstract\nBlood methanol concentrations in normal adult subjects administered abuse doses of aspartame. · 1981 · https://pubmed.ncbi.nlm.nih.gov/7230276/ · DOI 10.1080/15287398109529979","model_system":"Thirty adults across dose cohorts.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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