{"id":"a8cdaaae-1b3c-5176-9f89-13bc453c5b40","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-folate-methanol-context","predicate":"modulates_downstream_exposure_to","statement":"Four cynomolgus monkeys had higher labeled blood formate after 900 ppm methanol inhalation when folate-deficient than before depletion, while tracer-derived concentrations remained below endogenous formate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"44a4d788-cf2e-5e14-99c5-06094f9d07af","mechanism_event_label":"Downstream handling depends on nutrient state and exposure route.","subject":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"object":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"44a4d788-cf2e-5e14-99c5-06094f9d07af","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-folate-methanol-context-event","event_type":"observed_relationship","label":"Downstream handling depends on nutrient state and exposure route.","description":"Four cynomolgus monkeys had higher labeled blood formate after 900 ppm methanol inhalation when folate-deficient than before depletion, while tracer-derived concentrations remained below endogenous formate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e176a266-711e-5469-89da-3fb2d3f244ad","slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1fc1fa42-fcb5-5983-9a31-114703dc64c4","slug":"formate","display_name":"Formate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"96bbf620-a0f1-506b-9f01-a4f1ea1224a9","slug":"methanol","display_name":"Methanol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Two-hour lung-only labeled-methanol exposure; repeated after diet-induced folate deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This was not aspartame feeding, and does not show aspartame causes folate depletion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Downstream handling depends on nutrient state and exposure route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pharmacokinetics of inhaled [14C]methanol and methanol-derived [14C]formate in normal and folate-deficient cynomolgus monkeys. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7940538/ · DOI 10.1006/taap.1994.1202","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"73aa221a-913f-542f-a735-65792b283341","evidence_kind":"source_excerpt","locator":"Lines 154-160","start_line":154,"end_line":160,"excerpt":"## aspartame-folate-methanol-context\nDownstream handling depends on nutrient state and exposure route.\nFour cynomolgus monkeys had higher labeled blood formate after 900 ppm methanol inhalation when folate-deficient than before depletion, while tracer-derived concentrations remained below endogenous formate.\nModel: Two-hour lung-only labeled-methanol exposure; repeated after diet-induced folate deficiency.\nLimitations: This was not aspartame feeding, and does not show aspartame causes folate depletion.\nEvidence access: Primary abstract\nPharmacokinetics of inhaled [14C]methanol and methanol-derived [14C]formate in normal and folate-deficient cynomolgus monkeys. · 1994 · https://pubmed.ncbi.nlm.nih.gov/7940538/ · DOI 10.1006/taap.1994.1202","model_system":"Two-hour lung-only labeled-methanol exposure; repeated after diet-induced folate deficiency.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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