{"id":"5bcdbea6-beae-5298-96c3-1632e896b380","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-ester-cleavage","predicate":"yields_after_ester_hydrolysis","statement":"The intestinal study describes ester-bond hydrolysis of aspartame to Asp-Phe before further dipeptide digestion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7d01a230-d76c-53e0-a99e-3d4261b0a37f","mechanism_event_label":"Removing the methyl ester is separate from splitting the peptide.","subject":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"object":{"id":"4cf892ac-f3f0-5e54-994e-509758cb3ce3","slug":"alpha-l-aspartyl-l-phenylalanine","display_name":"Alpha-L-aspartyl-L-phenylalanine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"7d01a230-d76c-53e0-a99e-3d4261b0a37f","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-ester-cleavage-event","event_type":"observed_relationship","label":"Removing the methyl ester is separate from splitting the peptide.","description":"The intestinal study describes ester-bond hydrolysis of aspartame to Asp-Phe before further dipeptide digestion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4cf892ac-f3f0-5e54-994e-509758cb3ce3","slug":"alpha-l-aspartyl-l-phenylalanine","display_name":"Alpha-L-aspartyl-L-phenylalanine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"96bbf620-a0f1-506b-9f01-a4f1ea1224a9","slug":"methanol","display_name":"Methanol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reaction background and human intestinal mucosal enzyme experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This record does not assign the initial esterase to an unverified gene.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Removing the methyl ester is separate from splitting the peptide.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Intestinal hydrolysis of aspartylphenylalanine--the metabolic product of aspartame. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3743970/ · DOI 10.1016/0016-5085(86)90697-9","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5fad1345-682d-513f-9e96-635d3a9620b8","evidence_kind":"source_excerpt","locator":"Lines 18-24","start_line":18,"end_line":24,"excerpt":"## aspartame-ester-cleavage\nRemoving the methyl ester is separate from splitting the peptide.\nThe intestinal study describes ester-bond hydrolysis of aspartame to Asp-Phe before further dipeptide digestion.\nModel: Reaction background and human intestinal mucosal enzyme experiments.\nLimitations: This record does not assign the initial esterase to an unverified gene.\nEvidence access: Primary abstract\nIntestinal hydrolysis of aspartylphenylalanine--the metabolic product of aspartame. · 1986 · https://pubmed.ncbi.nlm.nih.gov/3743970/ · DOI 10.1016/0016-5085(86)90697-9","model_system":"Reaction background and human intestinal mucosal enzyme experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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