Component

Cellular zinc influx

Experimentally measured zinc entry; assays distinguish flux from total zinc content.

6 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. CNNM2 coexpression increased TRPM7-dependent zinc influx, and the TRPM7 E1047K pore mutant prevented this increase.

    CNNM2 → Cellular zinc influx source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    A protein associated with Mg homeostasis regulates a Zn-permeable channel in culture; nutritional co-dependence was not tested.
    evidence-system
    Coexpression, TRPM7 knockout and pore-inactivation tests
    experimental_model
    Coexpression, TRPM7 knockout and pore-inactivation tests
    limitations
    Zinc is a divalent-flux reporter here; this does not establish CNNM2 as a magnesium pore or dietary Mg-to-Zn dependence.
    nutrient_topic
    Magnesium research collection; topical membership is not evidence of a direct dietary effect. · Magnesium
    organism
    Human proteins and human-derived cells
    plain_language
    CNNM2 can regulate entry through a separate channel whose pore carries the ion.
    primary_references
    [bai-2021-cnnm-trpm7] CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells (2021). https://pmc.ncbi.nlm.nih.gov/articles/PMC8726484/ DOI: 10.1371/journal.pbio.3001496
    tissue
    HEK293-family cells; supporting HAP1 cells
    tissue_or_cell_type
    HEK293-family cells; supporting HAP1 cells

    Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17) · lines 1151–1163

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Coexpression, TRPM7 knockout and pore-inactivation tests · source_derived_draft · unverified_draft

    ### cnnm2-stimulates-trpm7-zinc-influx CNNM2 coexpression increased TRPM7-dependent zinc influx, and the TRPM7 E1047K pore mutant prevented this increase. Condition category: normal nutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect. plain_language: CNNM2 can regulate entry through a separate channel whose pore carries the ion. organism: Human proteins and human-derived cells tissue_or_cell_type: HEK293-family cells; supporting HAP1 cells experimental_model: Coexpression, TRPM7 knockout and pore-inactivation tests limitations: Zinc is a divalent-flux reporter here; this does not establish CNNM2 as a magnesium pore or dietary Mg-to-Zn dependence. cross_nutrient: A protein associated with Mg homeostasis regulates a Zn-permeable channel in culture; nutritional co-dependence was not tested. evidence-system: Coexpression, TRPM7 knockout and pore-inactivation tests tissue: HEK293-family cells; supporting HAP1 cells [bai-2021-cnnm-trpm7] CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells (2021). https://pmc.ncbi.nlm.nih.gov/articles/PMC8726484/ DOI: 10.1371/journal.pbio.3001496
    Complete structured claim and evidence

  2. Mouse ZIP14 expression in Xenopus oocytes supported saturable zinc uptake with an apparent half-maximal zinc concentration near 2 micromolar.

    Mouse ZIP14 (Slc39a14) → Cellular zinc influx source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    experimental_model
    RNA-injected Xenopus oocytes and 65Zn uptake
    exposure
    Zinc concentration-response curve under the study transport conditions.
    limitations
    The apparent affinity belongs to this assay, not a serum zinc cutoff; protein is the mouse ortholog.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Mouse protein in Xenopus laevis oocytes
    plain_language
    Mouse ZIP14 directly carried zinc in the oocyte assay.
    primary_references
    [zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010
    tissue_or_cell_type
    Oocyte plasma membrane

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 479–490

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · RNA-injected Xenopus oocytes and 65Zn uptake · source_derived_draft · unverified_draft

    ### zinc-trans-zip14-zinc-influx Mouse ZIP14 expression in Xenopus oocytes supported saturable zinc uptake with an apparent half-maximal zinc concentration near 2 micromolar. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Mouse ZIP14 directly carried zinc in the oocyte assay. organism: Mouse protein in Xenopus laevis oocytes tissue_or_cell_type: Oocyte plasma membrane experimental_model: RNA-injected Xenopus oocytes and 65Zn uptake limitations: The apparent affinity belongs to this assay, not a serum zinc cutoff; protein is the mouse ortholog. exposure: Zinc concentration-response curve under the study transport conditions. cross_nutrient: false [zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010
    Complete structured claim and evidence

  3. Expression of mouse ZIP4 increased zinc influx into transfected cells.

    Mouse ZIP4 (Slc39a4) → Cellular zinc influx source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    experimental_model
    Transfected mammalian cells
    exposure
    ZIP4 expression versus controls; abstract does not specify zinc concentration.
    limitations
    Transport in engineered cells establishes capacity, not the fraction of human dietary zinc absorption.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Mouse protein in cultured mammalian cells
    plain_language
    Mouse ZIP4 can bring zinc into cells.
    primary_references
    [zinc-trans-12801924] The acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice. (2003). https://pubmed.ncbi.nlm.nih.gov/12801924/ DOI: 10.1074/jbc.m305000200
    tissue_or_cell_type
    Cell plasma membrane

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 219–230

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Transfected mammalian cells · source_derived_draft · unverified_draft

    ### zinc-trans-zip4-influx Expression of mouse ZIP4 increased zinc influx into transfected cells. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Mouse ZIP4 can bring zinc into cells. organism: Mouse protein in cultured mammalian cells tissue_or_cell_type: Cell plasma membrane experimental_model: Transfected mammalian cells limitations: Transport in engineered cells establishes capacity, not the fraction of human dietary zinc absorption. exposure: ZIP4 expression versus controls; abstract does not specify zinc concentration. cross_nutrient: false [zinc-trans-12801924] The acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice. (2003). https://pubmed.ncbi.nlm.nih.gov/12801924/ DOI: 10.1074/jbc.m305000200
    Complete structured claim and evidence

  4. Mouse ZIP5 expression increased cellular Zn(II) uptake and showed selectivity for zinc among the potential substrates tested.

    Mouse ZIP5 (Slc39a5) → Cellular zinc influx source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    experimental_model
    Heterologous mouse ZIP5 expression
    exposure
    Expression and substrate tests; abstract does not specify concentrations.
    limitations
    Selectivity is bounded by the tested panel and assay; this cannot exclude every other substrate in other systems.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Mouse protein in cultured cells
    plain_language
    Mouse ZIP5 can move zinc into cells.
    primary_references
    [zinc-trans-15322118] The mammalian Zip5 protein is a zinc transporter that localizes to the basolateral surface of polarized cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15322118/ DOI: 10.1074/jbc.m408361200
    tissue_or_cell_type
    Cultured-cell plasma membrane

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 362–373

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Heterologous mouse ZIP5 expression · source_derived_draft · unverified_draft

    ### zinc-trans-zip5-influx Mouse ZIP5 expression increased cellular Zn(II) uptake and showed selectivity for zinc among the potential substrates tested. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Mouse ZIP5 can move zinc into cells. organism: Mouse protein in cultured cells tissue_or_cell_type: Cultured-cell plasma membrane experimental_model: Heterologous mouse ZIP5 expression limitations: Selectivity is bounded by the tested panel and assay; this cannot exclude every other substrate in other systems. exposure: Expression and substrate tests; abstract does not specify concentrations. cross_nutrient: false [zinc-trans-15322118] The mammalian Zip5 protein is a zinc transporter that localizes to the basolateral surface of polarized cells. (2004). https://pubmed.ncbi.nlm.nih.gov/15322118/ DOI: 10.1074/jbc.m408361200
    Complete structured claim and evidence

  5. A tenfold molar excess of iron inhibited radiolabeled zinc uptake in HEK293T cells expressing rat ZIP8.

    Ferrous iron → Cellular zinc influx source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    true
    experimental_model
    Rat ZIP8-expressing HEK293T radiotracer assay
    exposure
    2 micromolar labeled zinc and tenfold unlabeled iron excess; uptake medium included ascorbate.
    limitations
    Transport competition in cells is not a universal dietary zinc/iron ratio or a prediction for mixed meals.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Rat protein in human cells
    plain_language
    Iron reduced zinc entry through this transporter system under the tested culture conditions.
    primary_references
    [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
    tissue_or_cell_type
    HEK293T cells

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 440–451

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat ZIP8-expressing HEK293T radiotracer assay · source_derived_draft · unverified_draft

    ### zinc-trans-zip8-iron-inhibits-zinc A tenfold molar excess of iron inhibited radiolabeled zinc uptake in HEK293T cells expressing rat ZIP8. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Iron reduced zinc entry through this transporter system under the tested culture conditions. organism: Rat protein in human cells tissue_or_cell_type: HEK293T cells experimental_model: Rat ZIP8-expressing HEK293T radiotracer assay limitations: Transport competition in cells is not a universal dietary zinc/iron ratio or a prediction for mixed meals. exposure: 2 micromolar labeled zinc and tenfold unlabeled iron excess; uptake medium included ascorbate. cross_nutrient: true [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
    Complete structured claim and evidence

  6. Rat ZIP8 expression in HEK293T cells increased radiolabeled zinc uptake by about 40% compared with empty-vector controls.

    Rat ZIP8 (Slc39a8) → Cellular zinc influx source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    experimental_model
    Rat ZIP8 transfection and 65Zn uptake
    exposure
    2 micromolar radiolabeled zinc for 1 hour, 48 hours after transfection.
    limitations
    Overexpression effect; it does not quantify native human tissue flux.
    nutrient_topic
    Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
    organism
    Rat protein in human HEK293T cells
    plain_language
    Rat ZIP8 increased zinc entry when expressed in human-derived cells.
    primary_references
    [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
    tissue_or_cell_type
    Cultured-cell plasma membrane

    Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 427–438

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat ZIP8 transfection and 65Zn uptake · source_derived_draft · unverified_draft

    ### zinc-trans-zip8-zinc-influx Rat ZIP8 expression in HEK293T cells increased radiolabeled zinc uptake by about 40% compared with empty-vector controls. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Rat ZIP8 increased zinc entry when expressed in human-derived cells. organism: Rat protein in human HEK293T cells tissue_or_cell_type: Cultured-cell plasma membrane experimental_model: Rat ZIP8 transfection and 65Zn uptake limitations: Overexpression effect; it does not quantify native human tissue flux. exposure: 2 micromolar radiolabeled zinc for 1 hour, 48 hours after transfection. cross_nutrient: false [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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