Component
Rat ZIP8 (Slc39a8)
Rattus norvegicus ZIP8 multimetal transporter protein.
3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.
How nutrients influence it
Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.
Other things that act on it
Enzymes, hormones, genes, and other components with a recorded effect. These are not nutrients, so they do not count toward the arrows above. Each finding names the chapter that recorded it.
How nutrients reach it in more than one step
Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.
Tracing routes…
What it does
Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.
What it acts on
Rat ZIP8 expression in HEK293T cells increased radiolabeled zinc uptake by about 40% compared with empty-vector controls.
Experimental context and source evidence
- cross_nutrient
- false
- experimental_model
- Rat ZIP8 transfection and 65Zn uptake
- exposure
- 2 micromolar radiolabeled zinc for 1 hour, 48 hours after transfection.
- limitations
- Overexpression effect; it does not quantify native human tissue flux.
- nutrient_topic
- Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
- organism
- Rat protein in human HEK293T cells
- plain_language
- Rat ZIP8 increased zinc entry when expressed in human-derived cells.
- primary_references
- [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
- tissue_or_cell_type
- Cultured-cell plasma membrane
Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 427–438
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat ZIP8 transfection and 65Zn uptake · source_derived_draft · unverified_draft
### zinc-trans-zip8-zinc-influx Rat ZIP8 expression in HEK293T cells increased radiolabeled zinc uptake by about 40% compared with empty-vector controls. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Rat ZIP8 increased zinc entry when expressed in human-derived cells. organism: Rat protein in human HEK293T cells tissue_or_cell_type: Cultured-cell plasma membrane experimental_model: Rat ZIP8 transfection and 65Zn uptake limitations: Overexpression effect; it does not quantify native human tissue flux. exposure: 2 micromolar radiolabeled zinc for 1 hour, 48 hours after transfection. cross_nutrient: false [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
Complete structured claim and evidence
Where it participates (unsigned role)
A tenfold molar excess of iron inhibited radiolabeled zinc uptake in HEK293T cells expressing rat ZIP8.
Experimental context and source evidence
- cross_nutrient
- true
- experimental_model
- Rat ZIP8-expressing HEK293T radiotracer assay
- exposure
- 2 micromolar labeled zinc and tenfold unlabeled iron excess; uptake medium included ascorbate.
- limitations
- Transport competition in cells is not a universal dietary zinc/iron ratio or a prediction for mixed meals.
- nutrient_topic
- Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
- organism
- Rat protein in human cells
- plain_language
- Iron reduced zinc entry through this transporter system under the tested culture conditions.
- primary_references
- [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
- tissue_or_cell_type
- HEK293T cells
Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 440–451
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat ZIP8-expressing HEK293T radiotracer assay · source_derived_draft · unverified_draft
### zinc-trans-zip8-iron-inhibits-zinc A tenfold molar excess of iron inhibited radiolabeled zinc uptake in HEK293T cells expressing rat ZIP8. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Iron reduced zinc entry through this transporter system under the tested culture conditions. organism: Rat protein in human cells tissue_or_cell_type: HEK293T cells experimental_model: Rat ZIP8-expressing HEK293T radiotracer assay limitations: Transport competition in cells is not a universal dietary zinc/iron ratio or a prediction for mixed meals. exposure: 2 micromolar labeled zinc and tenfold unlabeled iron excess; uptake medium included ascorbate. cross_nutrient: true [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
Complete structured claim and evidenceA tenfold molar excess of zinc inhibited iron uptake by more than 90% in rat ZIP8-expressing HEK293T cells under the study conditions.
Experimental context and source evidence
- cross_nutrient
- true
- experimental_model
- Rat ZIP8-expressing HEK293T radiotracer assay
- exposure
- 2 micromolar labeled iron with ascorbate and tenfold unlabeled zinc excess.
- limitations
- The assay contains reduction chemistry and soluble ions; this is not direct evidence for the magnitude of human meal-iron inhibition.
- nutrient_topic
- Zinc research collection; topical membership is not evidence of a direct dietary effect. · Zinc
- organism
- Rat protein in human cells
- plain_language
- Zinc strongly reduced iron entry in this cultured transport system.
- primary_references
- [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
- tissue_or_cell_type
- HEK293T cells
Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17) · lines 453–464
AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Rat ZIP8-expressing HEK293T radiotracer assay · source_derived_draft · unverified_draft
### zinc-trans-zip8-zinc-inhibits-iron A tenfold molar excess of zinc inhibited iron uptake by more than 90% in rat ZIP8-expressing HEK293T cells under the study conditions. Condition category: normal nutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect. plain_language: Zinc strongly reduced iron entry in this cultured transport system. organism: Rat protein in human cells tissue_or_cell_type: HEK293T cells experimental_model: Rat ZIP8-expressing HEK293T radiotracer assay limitations: The assay contains reduction chemistry and soluble ions; this is not direct evidence for the magnitude of human meal-iron inhibition. exposure: 2 micromolar labeled iron with ascorbate and tenfold unlabeled zinc excess. cross_nutrient: true [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284
Complete structured claim and evidence
The events it takes part in
A mechanism often involves more than two components. These are the full events, with every participant and its role.
Situations it appears in
Low-supply and faulty-machinery situations recorded in the chapters where this component plays a part.
In the sources
Preserved passages that mention this component, quoted exactly. Open one to read it in context.
Open hypotheses
Proposed ideas that involve this component. They are labeled as hypotheses and do not change any recorded statement.
This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.