{"id":"c4f7d9c7-cee4-5277-8a2f-61338240beb6","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip8-zinc-influx","predicate":"increases","statement":"Rat ZIP8 expression in HEK293T cells increased radiolabeled zinc uptake by about 40% compared with empty-vector controls.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b92a6967-c165-5060-99c9-9709bec4af8b","mechanism_event_label":"Rat ZIP8 increased zinc entry when expressed in human-derived cells.","subject":{"id":"ba09e4ed-8b9c-5cd9-bea8-6f8527090978","slug":"rat-slc39a8","display_name":"Rat ZIP8 (Slc39a8)","entity_type_key":"protein"},"object":{"id":"39158631-eef1-5e08-96a3-385ba1b5acf3","slug":"cellular-zinc-influx","display_name":"Cellular zinc influx","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b92a6967-c165-5060-99c9-9709bec4af8b","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip8-zinc-influx-event","event_type":"biochemical_relationship","label":"Rat ZIP8 increased zinc entry when expressed in human-derived cells.","description":"Rat ZIP8 expression in HEK293T cells increased radiolabeled zinc uptake by about 40% compared with empty-vector controls.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ba09e4ed-8b9c-5cd9-bea8-6f8527090978","slug":"rat-slc39a8","display_name":"Rat ZIP8 (Slc39a8)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"39158631-eef1-5e08-96a3-385ba1b5acf3","slug":"cellular-zinc-influx","display_name":"Cellular zinc influx","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat ZIP8 transfection and 65Zn uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"2 micromolar radiolabeled zinc for 1 hour, 48 hours after transfection.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Overexpression effect; it does not quantify native human tissue flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rat protein in human HEK293T cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Rat ZIP8 increased zinc entry when expressed in human-derived cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured-cell plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1147160f-2517-5681-8db4-ed91a88f87e4","evidence_kind":"source_excerpt","locator":"Lines 427-438","start_line":427,"end_line":438,"excerpt":"### zinc-trans-zip8-zinc-influx\nRat ZIP8 expression in HEK293T cells increased radiolabeled zinc uptake by about 40% compared with empty-vector controls.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Rat ZIP8 increased zinc entry when expressed in human-derived cells.\norganism: Rat protein in human HEK293T cells\ntissue_or_cell_type: Cultured-cell plasma membrane\nexperimental_model: Rat ZIP8 transfection and 65Zn uptake\nlimitations: Overexpression effect; it does not quantify native human tissue flux.\nexposure: 2 micromolar radiolabeled zinc for 1 hour, 48 hours after transfection.\ncross_nutrient: false\n[zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284","model_system":"Rat ZIP8 transfection and 65Zn uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-22898811] ZIP8 is an iron and zinc transporter whose cell-surface expression is up-regulated by cellular iron loading. (2012). https://pubmed.ncbi.nlm.nih.gov/22898811/ DOI: 10.1074/jbc.m112.367284","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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