{"id":"42e5f012-ce9f-5fe6-86fe-56eae9f22897","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:cnnm2-stimulates-trpm7-zinc-influx","predicate":"stimulates","statement":"CNNM2 coexpression increased TRPM7-dependent zinc influx, and the TRPM7 E1047K pore mutant prevented this increase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"a041c978-5e98-51bb-a718-b0347ab8b243","mechanism_event_label":"CNNM2 can regulate entry through a separate channel whose pore carries the ion.","subject":{"id":"f98d380e-9285-5154-9e0b-6456f4a434ef","slug":"cnnm2","display_name":"CNNM2","entity_type_key":"protein"},"object":{"id":"39158631-eef1-5e08-96a3-385ba1b5acf3","slug":"cellular-zinc-influx","display_name":"Cellular zinc influx","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a041c978-5e98-51bb-a718-b0347ab8b243","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:cnnm2-stimulates-trpm7-zinc-influx-event","event_type":"biochemical_relationship","label":"CNNM2 can regulate entry through a separate channel whose pore carries the ion.","description":"CNNM2 coexpression increased TRPM7-dependent zinc influx, and the TRPM7 E1047K pore mutant prevented this increase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"16ade5d1-e269-5904-9ab1-b285509b7aef","slug":"trpm7","display_name":"TRPM7","entity_type_key":"protein"},"role":"required-channel-pore","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"measured-cargo","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"homeostasis-context","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f98d380e-9285-5154-9e0b-6456f4a434ef","slug":"cnnm2","display_name":"CNNM2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"39158631-eef1-5e08-96a3-385ba1b5acf3","slug":"cellular-zinc-influx","display_name":"Cellular zinc influx","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"A protein associated with Mg homeostasis regulates a Zn-permeable channel in culture; nutritional co-dependence was not tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence-system","value_text":"Coexpression, TRPM7 knockout and pore-inactivation tests","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Coexpression, TRPM7 knockout and pore-inactivation tests","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Zinc is a divalent-flux reporter here; this does not establish CNNM2 as a magnesium pore or dietary Mg-to-Zn dependence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human proteins and human-derived cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"CNNM2 can regulate entry through a separate channel whose pore carries the ion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[bai-2021-cnnm-trpm7] CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells (2021). https://pmc.ncbi.nlm.nih.gov/articles/PMC8726484/ DOI: 10.1371/journal.pbio.3001496","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"HEK293-family cells; supporting HAP1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293-family cells; supporting HAP1 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9cfdb0f8-1ab7-511e-847e-db391090a5a9","evidence_kind":"source_excerpt","locator":"Lines 1151-1163","start_line":1151,"end_line":1163,"excerpt":"### cnnm2-stimulates-trpm7-zinc-influx\nCNNM2 coexpression increased TRPM7-dependent zinc influx, and the TRPM7 E1047K pore mutant prevented this increase.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: CNNM2 can regulate entry through a separate channel whose pore carries the ion.\norganism: Human proteins and human-derived cells\ntissue_or_cell_type: HEK293-family cells; supporting HAP1 cells\nexperimental_model: Coexpression, TRPM7 knockout and pore-inactivation tests\nlimitations: Zinc is a divalent-flux reporter here; this does not establish CNNM2 as a magnesium pore or dietary Mg-to-Zn dependence.\ncross_nutrient: A protein associated with Mg homeostasis regulates a Zn-permeable channel in culture; nutritional co-dependence was not tested.\nevidence-system: Coexpression, TRPM7 knockout and pore-inactivation tests\ntissue: HEK293-family cells; supporting HAP1 cells\n[bai-2021-cnnm-trpm7] CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells (2021). https://pmc.ncbi.nlm.nih.gov/articles/PMC8726484/ DOI: 10.1371/journal.pbio.3001496","model_system":"Coexpression, TRPM7 knockout and pore-inactivation tests","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [bai-2021-cnnm-trpm7] CNNM proteins selectively bind to the TRPM7 channel to stimulate divalent cation entry into cells (2021). https://pmc.ncbi.nlm.nih.gov/articles/PMC8726484/ DOI: 10.1371/journal.pbio.3001496","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}