{"id":"651565cd-19cb-5609-8a09-f490c63adcc0","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-influx","predicate":"increases","statement":"Expression of mouse ZIP4 increased zinc influx into transfected cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6949ca0d-a3f5-5887-a2f7-256810a640dc","mechanism_event_label":"Mouse ZIP4 can bring zinc into cells.","subject":{"id":"b2399154-5b80-5772-bf7c-d9d80aefb691","slug":"mouse-slc39a4","display_name":"Mouse ZIP4 (Slc39a4)","entity_type_key":"protein"},"object":{"id":"39158631-eef1-5e08-96a3-385ba1b5acf3","slug":"cellular-zinc-influx","display_name":"Cellular zinc influx","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6949ca0d-a3f5-5887-a2f7-256810a640dc","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip4-influx-event","event_type":"biochemical_relationship","label":"Mouse ZIP4 can bring zinc into cells.","description":"Expression of mouse ZIP4 increased zinc influx into transfected cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b2399154-5b80-5772-bf7c-d9d80aefb691","slug":"mouse-slc39a4","display_name":"Mouse ZIP4 (Slc39a4)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"39158631-eef1-5e08-96a3-385ba1b5acf3","slug":"cellular-zinc-influx","display_name":"Cellular zinc influx","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transfected mammalian cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ZIP4 expression versus controls; abstract does not specify zinc concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport in engineered cells establishes capacity, not the fraction of human dietary zinc absorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse protein in cultured mammalian cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Mouse ZIP4 can bring zinc into cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-12801924] The acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice. (2003). https://pubmed.ncbi.nlm.nih.gov/12801924/ DOI: 10.1074/jbc.m305000200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4c32ff01-a228-5e43-b26e-0f7696e07c72","evidence_kind":"source_excerpt","locator":"Lines 219-230","start_line":219,"end_line":230,"excerpt":"### zinc-trans-zip4-influx\nExpression of mouse ZIP4 increased zinc influx into transfected cells.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Mouse ZIP4 can bring zinc into cells.\norganism: Mouse protein in cultured mammalian cells\ntissue_or_cell_type: Cell plasma membrane\nexperimental_model: Transfected mammalian cells\nlimitations: Transport in engineered cells establishes capacity, not the fraction of human dietary zinc absorption.\nexposure: ZIP4 expression versus controls; abstract does not specify zinc concentration.\ncross_nutrient: false\n[zinc-trans-12801924] The acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice. (2003). https://pubmed.ncbi.nlm.nih.gov/12801924/ DOI: 10.1074/jbc.m305000200","model_system":"Transfected mammalian cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-12801924] The acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice. (2003). https://pubmed.ncbi.nlm.nih.gov/12801924/ DOI: 10.1074/jbc.m305000200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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