trans-Nasunin

evidence_span

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experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

Here the protective response preserved signaling rather than suppressing it.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 393–404

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-akt Nasunin prevented the challenge-associated loss of Akt phosphorylation. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Here the protective response preserved signaling rather than suppressing it. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

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experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

A differentiation marker was better maintained.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 354–365

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-alp Nasunin countered the challenge-associated decrease in the alkaline-phosphatase marker. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: A differentiation marker was better maintained. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

{"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"}

experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

Fewer cells entered the measured death pathway.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 315–326

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-apoptosis Pretreatment reduced apoptosis during t-BHP challenge. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Fewer cells entered the measured death pathway. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

{"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"}

experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

The measured matrix component was better retained.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 367–378

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-collagen Nasunin countered the challenge-associated decrease in collagen content. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The measured matrix component was better retained. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

{"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"}

experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

The cells retained their internal structural organization.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 380–391

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-cytoskeleton Nasunin prevented challenge-associated disruption of cytoskeleton organization. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The cells retained their internal structural organization. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

{"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"}

experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

The response includes a molecule shared with several nutrient-dependent defenses.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 328–339

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-gsh At 1 nM, nasunin increased intracellular glutathione in challenged cells. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The response includes a molecule shared with several nutrient-dependent defenses. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

{"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"}

experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

The oxidant readout decreased.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 341–352

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-ros Nasunin lowered intracellular ROS in challenged cells. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The oxidant readout decreased. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

{"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"}

experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

More challenged cells remained viable.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 302–313

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-survival Pretreatment increased viability during t-BHP challenge. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: More challenged cells remained viable. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

evidence_span

{"source_cache": "artifacts/nasunin-research/16848510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a", "start_char": 0, "end_char": 1308, "text_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a"}

experimental_model

Oral isomer pharmacokinetics with chromatographic metabolite analysis

exposure

Oral purified cis/trans nasunin; plasma follow-up to 8 h

limitations

Dose-normalized Cmax/AUC comparison is not absolute oral bioavailability. Nondetection applies to the tested analytes, method and window; no human or blood-brain-barrier conclusion.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Rats

plain_language

The second form was also detected without prior deacylation.

primary_references

[nasunin-p16848510] Gastrointestinal uptake of nasunin, acylated anthocyanin in eggplant. (2006). https://pubmed.ncbi.nlm.nih.gov/16848510/ DOI: 10.1021/jf060238s

tissue_or_cell_type

Plasma and urine

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 731–742

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oral isomer pharmacokinetics with chromatographic metabolite analysis · source_derived_draft · unverified_draft

### nasunin-trans-uptake Oral trans-nasunin also peaked as intact acylated pigment at 15 minutes. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The second form was also detected without prior deacylation. organism: Rats tissue_or_cell_type: Plasma and urine experimental_model: Oral isomer pharmacokinetics with chromatographic metabolite analysis limitations: Dose-normalized Cmax/AUC comparison is not absolute oral bioavailability. Nondetection applies to the tested analytes, method and window; no human or blood-brain-barrier conclusion. exposure: Oral purified cis/trans nasunin; plasma follow-up to 8 h evidence_span: {"source_cache": "artifacts/nasunin-research/16848510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a", "start_char": 0, "end_char": 1308, "text_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a"} [nasunin-p16848510] Gastrointestinal uptake of nasunin, acylated anthocyanin in eggplant. (2006). https://pubmed.ncbi.nlm.nih.gov/16848510/ DOI: 10.1021/jf060238s

evidence_span

{"source_cache": "artifacts/nasunin-research/16848510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a", "start_char": 0, "end_char": 1308, "text_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a"}

experimental_model

Oral isomer pharmacokinetics with chromatographic metabolite analysis

exposure

Oral purified cis/trans nasunin; plasma follow-up to 8 h

limitations

Dose-normalized Cmax/AUC comparison is not absolute oral bioavailability. Nondetection applies to the tested analytes, method and window; no human or blood-brain-barrier conclusion.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Rats

plain_language

Neither isomer showed a clear advantage in this comparison.

primary_references

[nasunin-p16848510] Gastrointestinal uptake of nasunin, acylated anthocyanin in eggplant. (2006). https://pubmed.ncbi.nlm.nih.gov/16848510/ DOI: 10.1021/jf060238s

tissue_or_cell_type

Plasma and urine

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 744–755

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oral isomer pharmacokinetics with chromatographic metabolite analysis · source_derived_draft · unverified_draft

### nasunin-isomer-comparison Dose-normalized peak concentrations and areas under the curve did not significantly differ between isomers. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Neither isomer showed a clear advantage in this comparison. organism: Rats tissue_or_cell_type: Plasma and urine experimental_model: Oral isomer pharmacokinetics with chromatographic metabolite analysis limitations: Dose-normalized Cmax/AUC comparison is not absolute oral bioavailability. Nondetection applies to the tested analytes, method and window; no human or blood-brain-barrier conclusion. exposure: Oral purified cis/trans nasunin; plasma follow-up to 8 h evidence_span: {"source_cache": "artifacts/nasunin-research/16848510.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a", "start_char": 0, "end_char": 1308, "text_sha256": "72244518913802f8f2018e3e6e7add9914294e49c58b9e9733c26a550e86479a"} [nasunin-p16848510] Gastrointestinal uptake of nasunin, acylated anthocyanin in eggplant. (2006). https://pubmed.ncbi.nlm.nih.gov/16848510/ DOI: 10.1021/jf060238s

evidence_span

{"source_cache": "artifacts/nasunin-research/16302764.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e", "start_char": 0, "end_char": 759, "text_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e"}

experimental_model

Purification, mass spectrometry and NMR

exposure

Acidified methanolic extract; room-light exposure

limitations

Cis/trans refers to the p-coumaroyl moiety. Interconversion during handling can change the tested preparation; this is not human metabolism.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Solanum melongena

plain_language

Sample illumination can alter the isomer mixture.

primary_references

[nasunin-p16302764] Nasunin from eggplant consists of cis-trans isomers of delphinidin 3-[4-(p-coumaroyl)-L-rhamnosyl (1-->6)glucopyranoside]-5-glucopyranoside. (2005). https://pubmed.ncbi.nlm.nih.gov/16302764/ DOI: 10.1021/jf051841y

tissue_or_cell_type

Eggplant peel pigments

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 120–131

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purification, mass spectrometry and NMR · source_derived_draft · unverified_draft

### nasunin-photoisomerization The isolated cis and trans forms interconverted under room light. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: Sample illumination can alter the isomer mixture. organism: Solanum melongena tissue_or_cell_type: Eggplant peel pigments experimental_model: Purification, mass spectrometry and NMR limitations: Cis/trans refers to the p-coumaroyl moiety. Interconversion during handling can change the tested preparation; this is not human metabolism. exposure: Acidified methanolic extract; room-light exposure evidence_span: {"source_cache": "artifacts/nasunin-research/16302764.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e", "start_char": 0, "end_char": 759, "text_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e"} [nasunin-p16302764] Nasunin from eggplant consists of cis-trans isomers of delphinidin 3-[4-(p-coumaroyl)-L-rhamnosyl (1-->6)glucopyranoside]-5-glucopyranoside. (2005). https://pubmed.ncbi.nlm.nih.gov/16302764/ DOI: 10.1021/jf051841y

evidence_span

{"source_cache": "artifacts/nasunin-research/16302764.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e", "start_char": 0, "end_char": 759, "text_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e"}

experimental_model

Purification, mass spectrometry and NMR

exposure

Acidified methanolic extract; room-light exposure

limitations

Cis/trans refers to the p-coumaroyl moiety. Interconversion during handling can change the tested preparation; this is not human metabolism.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Solanum melongena

plain_language

The trans form has its own database identity.

primary_references

[nasunin-p16302764] Nasunin from eggplant consists of cis-trans isomers of delphinidin 3-[4-(p-coumaroyl)-L-rhamnosyl (1-->6)glucopyranoside]-5-glucopyranoside. (2005). https://pubmed.ncbi.nlm.nih.gov/16302764/ DOI: 10.1021/jf051841y

tissue_or_cell_type

Eggplant peel pigments

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 107–118

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Purification, mass spectrometry and NMR · source_derived_draft · unverified_draft

### nasunin-trans-identity A trans-p-coumaroyl isomer was separately identified. Condition category: normal nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The trans form has its own database identity. organism: Solanum melongena tissue_or_cell_type: Eggplant peel pigments experimental_model: Purification, mass spectrometry and NMR limitations: Cis/trans refers to the p-coumaroyl moiety. Interconversion during handling can change the tested preparation; this is not human metabolism. exposure: Acidified methanolic extract; room-light exposure evidence_span: {"source_cache": "artifacts/nasunin-research/16302764.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e", "start_char": 0, "end_char": 759, "text_sha256": "4a79be9e5b34aba2c189fcde3f22fe9142f8b87408f33caeefc9283841ec894e"} [nasunin-p16302764] Nasunin from eggplant consists of cis-trans isomers of delphinidin 3-[4-(p-coumaroyl)-L-rhamnosyl (1-->6)glucopyranoside]-5-glucopyranoside. (2005). https://pubmed.ncbi.nlm.nih.gov/16302764/ DOI: 10.1021/jf051841y

availability_state

machinery_impairment Imported condition classification; unverified.

evidence_span

{"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"}

experimental_model

Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition

exposure

Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM

limitations

Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.

nutrient_topic

Nasunin research collection; topical membership is not evidence of a direct dietary effect. · Nasunin

organism

Mouse MC3T3-E1 cells

plain_language

The pathway must remain functional for this observed protection.

primary_references

[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007

tissue_or_cell_type

Survival, differentiation markers, redox state and Akt signaling

trigger_kind

machinery_impairment Imported condition classification; unverified.

Nasunin: identity, redox chemistry and nutrient connections (2026-09-17) · lines 406–417

AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition · source_derived_draft · unverified_draft

### nasunin-bone-pi3k-dependence LY294002 reversed nasunin-associated protection and worsened t-BHP cytotoxicity. Condition category: machinery_impairment nutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect. plain_language: The pathway must remain functional for this observed protection. organism: Mouse MC3T3-E1 cells tissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling experimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition limitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding. exposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM evidence_span: {"source_cache": "artifacts/nasunin-research/bone2016.publisher-abstract.txt", "locator": "Primary publisher abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc", "start_char": 0, "end_char": 1187, "text_sha256": "83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc"} [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007