{"id":"8ca71ff7-4634-5ee9-a91a-c550900fefeb","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-bone-apoptosis","predicate":"reduced","statement":"Pretreatment reduced apoptosis during t-BHP challenge.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ff5c4884-075a-5ae5-aa5b-a10236976b1f","mechanism_event_label":"Fewer cells entered the measured death pathway.","subject":{"id":"6c0572c8-5407-5192-b802-7f8c8be12293","slug":"trans-nasunin","display_name":"trans-Nasunin","entity_type_key":"small_molecule"},"object":{"id":"ff58b72d-be7f-5e2c-ad4b-c0e81b1f58e7","slug":"osteoblast-apoptosis","display_name":"Osteoblast apoptosis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ff5c4884-075a-5ae5-aa5b-a10236976b1f","stable_key":"e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff:nasunin-bone-apoptosis-event","event_type":"biochemical_relationship","label":"Fewer cells entered the measured death pathway.","description":"Pretreatment reduced apoptosis during t-BHP challenge.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cb56e100-bcb1-5f49-9b0c-98e10a9a73bb","slug":"tert-butyl-hydroperoxide","display_name":"tert-Butyl hydroperoxide / t-BHP","entity_type_key":"small_molecule"},"role":"challenge","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6c0572c8-5407-5192-b802-7f8c8be12293","slug":"trans-nasunin","display_name":"trans-Nasunin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ff58b72d-be7f-5e2c-ad4b-c0e81b1f58e7","slug":"osteoblast-apoptosis","display_name":"Osteoblast apoptosis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/nasunin-research/bone2016.publisher-abstract.txt\", \"locator\": \"Primary publisher abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc\", \"start_char\": 0, \"end_char\": 1187, \"text_sha256\": \"83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Nasunin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nasunin","display_name":"Nasunin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse MC3T3-E1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Fewer cells entered the measured death pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Survival, differentiation markers, redox state and Akt signaling","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"7d90a5be-068e-5280-9059-b5e771a9ad93","evidence_kind":"source_excerpt","locator":"Lines 315-326","start_line":315,"end_line":326,"excerpt":"### nasunin-bone-apoptosis\nPretreatment reduced apoptosis during t-BHP challenge.\nCondition category: normal\nnutrient_topic: Nasunin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Fewer cells entered the measured death pathway.\norganism: Mouse MC3T3-E1 cells\ntissue_or_cell_type: Survival, differentiation markers, redox state and Akt signaling\nexperimental_model: Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition\nlimitations: Cell-line protection does not establish bone-density or fracture benefits in humans. PI3K inhibition supports pathway involvement, not direct nasunin binding.\nexposure: Purified trans-nasunin pretreatment 0.1 nM-1 micromolar; t-BHP 250 micromolar for 3 h; redox/function experiments at 1 nM\nevidence_span: {\"source_cache\": \"artifacts/nasunin-research/bone2016.publisher-abstract.txt\", \"locator\": \"Primary publisher abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc\", \"start_char\": 0, \"end_char\": 1187, \"text_sha256\": \"83d6dc95bb4cccd5028a3908a5a44639274f59f18e1cea4b13f6133b9fe726dc\"}\n[nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007","model_system":"Oxidant-challenged osteoblast-like cells with pharmacological pathway inhibition","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [nasunin-pbone2016] Nasunin, a new player in the field of osteoblast protection against oxidative stress (2016). https://air.unimi.it/handle/2434/378237 DOI: 10.1016/j.jff.2016.03.007","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2c43df19-ccb0-58f1-b9a4-bc92cda6de5a","stable_key":"import-e1af6d55-ced5-56c9-9a2d-8cc2ae6ec3ff","title":"Nasunin: identity, redox chemistry and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"56552c208433294bf9a6c95513d981dbe4d00fd8336c121816d7b8bd6604d50e","revision_id":"b0296d49-32d7-52dc-a23c-5e862da0fba3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}