Roundup MON52276, tested glyphosate formulation

evidence_access

Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.

experimental_contrast

{"intervention": "roundup-mon52276 at the reported tested concentration", "comparator": "Matched culture without formulation", "endpoint": "MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.", "effect_direction": "no_detected_change", "combination": "single", "conditions": []} Explicit extracted experimental comparison; source-derived draft.

experimental_model

Bacterial culture comparison separate from the rat study.

interpretation_status

Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.

limitations

Specific formulation and concentration comparison, not universal absence or presence of inhibition.

plain_language

MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.

primary_references

Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/

source_locator

Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.

Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 39–39

Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Bacterial culture comparison separate from the rat study. · source_derived_draft · unverified_draft

**Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)

evidence_access

Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.

experimental_model

Female Sprague–Dawley rats; 0.5, 50 and 175 mg/kg/day glyphosate-equivalent groups; 12/group.

interpretation_status

Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.

limitations

Study-level direction; no unsupported per-dose significance claim. Pool accumulation is not increased downstream flux.

plain_language

The 90-day roundup-mon52276 study recorded cecal 3-dehydroshikimate accumulation consistent with pathway inhibition.

primary_references

Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/

source_locator

Reviewed reference lines 37-37; exact primary location described in quoted passage where extracted.

Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 37–37

Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Female Sprague–Dawley rats; 0.5, 50 and 175 mg/kg/day glyphosate-equivalent groups; 12/group. · source_derived_draft · unverified_draft

**An upstream pool can rise when flux is blocked.** Mesnage 2021 compared pure glyphosate and Roundup MON52276 in female Sprague–Dawley rats for 90 days at 0.5, 50 and 175 mg/kg/day glyphosate-equivalent exposures, with 12 animals per group. Cecal shikimate and DHS accumulation supported pathway inhibition; abundance alone was less informative. The collection records study-level accumulation rather than asserting every dose had an identical significant effect. Overall alpha-diversity differences were not significant (p=0.09), while beta diversity differed. Selected low-abundance taxa changed, and formulation-specific differences remained. Serum shikimate was undetected with the employed method, whose sensitivity and protein interference limit a zero-exposure interpretation. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)

evidence_access

Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.

experimental_model

Female Sprague–Dawley rats; 0.5, 50 and 175 mg/kg/day glyphosate-equivalent groups; 12/group.

interpretation_status

Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.

limitations

Study-level direction; no unsupported per-dose significance claim. Pool accumulation is not increased downstream flux.

plain_language

The 90-day roundup-mon52276 study recorded cecal shikimate accumulation consistent with pathway inhibition.

primary_references

Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/

source_locator

Reviewed reference lines 37-37; exact primary location described in quoted passage where extracted.

Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 37–37

Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Female Sprague–Dawley rats; 0.5, 50 and 175 mg/kg/day glyphosate-equivalent groups; 12/group. · source_derived_draft · unverified_draft

**An upstream pool can rise when flux is blocked.** Mesnage 2021 compared pure glyphosate and Roundup MON52276 in female Sprague–Dawley rats for 90 days at 0.5, 50 and 175 mg/kg/day glyphosate-equivalent exposures, with 12 animals per group. Cecal shikimate and DHS accumulation supported pathway inhibition; abundance alone was less informative. The collection records study-level accumulation rather than asserting every dose had an identical significant effect. Overall alpha-diversity differences were not significant (p=0.09), while beta diversity differed. Selected low-abundance taxa changed, and formulation-specific differences remained. Serum shikimate was undetected with the employed method, whose sensitivity and protein interference limit a zero-exposure interpretation. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)

evidence_access

Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.

experimental_model

Across glyphosate and MON52276 treatment groups in the 90-day rat study.

interpretation_status

Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.

limitations

Diversity null does not erase metabolomic differences or establish absence of biological effect.

plain_language

Overall alpha-diversity differences in the study were not significant (p=0.09).

primary_references

Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/

source_locator

Reviewed reference lines 37-37; exact primary location described in quoted passage where extracted.

Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 37–37

Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Across glyphosate and MON52276 treatment groups in the 90-day rat study. · source_derived_draft · unverified_draft

**An upstream pool can rise when flux is blocked.** Mesnage 2021 compared pure glyphosate and Roundup MON52276 in female Sprague–Dawley rats for 90 days at 0.5, 50 and 175 mg/kg/day glyphosate-equivalent exposures, with 12 animals per group. Cecal shikimate and DHS accumulation supported pathway inhibition; abundance alone was less informative. The collection records study-level accumulation rather than asserting every dose had an identical significant effect. Overall alpha-diversity differences were not significant (p=0.09), while beta diversity differed. Selected low-abundance taxa changed, and formulation-specific differences remained. Serum shikimate was undetected with the employed method, whose sensitivity and protein interference limit a zero-exposure interpretation. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)

evidence_access

Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.

experimental_model

90-day rat metabolomics; study-level observed pool change.

interpretation_status

Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.

limitations

Not direct host glutathione flux, nutrient depletion, or per-dose replicated effect.

plain_language

The rat glyphosate/formulation study recorded increased cecal cysteinylglycine.

primary_references

Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/

source_locator

Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.

Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 39–39

Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · 90-day rat metabolomics; study-level observed pool change. · source_derived_draft · unverified_draft

**Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)

evidence_access

Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.

experimental_model

90-day rat metabolomics; study-level observed pool change.

interpretation_status

Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.

limitations

Not direct host glutathione flux, nutrient depletion, or per-dose replicated effect.

plain_language

The rat glyphosate/formulation study recorded increased cecal gamma-glutamylglutamine.

primary_references

Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/

source_locator

Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.

Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 39–39

Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · 90-day rat metabolomics; study-level observed pool change. · source_derived_draft · unverified_draft

**Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)

evidence_access

Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.

experimental_model

90-day rat metabolomics; study-level observed pool change.

interpretation_status

Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.

limitations

Not direct host glutathione flux, nutrient depletion, or per-dose replicated effect.

plain_language

The rat glyphosate/formulation study recorded increased cecal valylglycine.

primary_references

Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/

source_locator

Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.

Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 39–39

Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · 90-day rat metabolomics; study-level observed pool change. · source_derived_draft · unverified_draft

**Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)