{"id":"e96e7ea0-7700-51ea-893e-f59ba418ce6f","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:culture-roundup-mon52276","predicate":"no_detected_change_in_recorded_experiment","statement":"MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"5d006cf0-e9d4-5534-ba7a-a68b5408d630","mechanism_event_label":"MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.","subject":{"id":"60ab6ff2-50fa-5f69-b092-9bd293a65a64","slug":"roundup-mon52276","display_name":"Roundup MON52276, tested glyphosate formulation","entity_type_key":"chemical_species"},"object":{"id":"48a9015b-3c49-555f-a0f7-07d468f1cf19","slug":"l-rhamnosus-growth","display_name":"Lactobacillus rhamnosus growth in formulation-comparison assays","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5d006cf0-e9d4-5534-ba7a-a68b5408d630","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:culture-roundup-mon52276-event","event_type":"experimental_observation","label":"MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.","description":"**Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"60ab6ff2-50fa-5f69-b092-9bd293a65a64","slug":"roundup-mon52276","display_name":"Roundup MON52276, tested glyphosate formulation","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"roundup-mon52276 at the reported tested concentration","sequence_order":0,"notes":""},{"entity":{"id":"48a9015b-3c49-555f-a0f7-07d468f1cf19","slug":"l-rhamnosus-growth","display_name":"Lactobacillus rhamnosus growth in formulation-comparison assays","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"roundup-mon52276 at the reported tested concentration\", \"comparator\": \"Matched culture without formulation\", \"endpoint\": \"MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Bacterial culture comparison separate from the rat study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Specific formulation and concentration comparison, not universal absence or presence of inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ddc72491-2344-57f8-a6ac-4d542637af49","evidence_kind":"source_excerpt","locator":"Lines 39-39","start_line":39,"end_line":39,"excerpt":"**Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","model_system":"Bacterial culture comparison separate from the rat study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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