Component

Lactobacillus rhamnosus growth in formulation-comparison assays

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Pure glyphosate did not inhibit growth under the cited GT+-sensitive test conditions.

    Glyphosate → Lactobacillus rhamnosus growth in formulation-comparison assays source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.
    experimental_contrast
    {"intervention": "glyphosate at the reported tested concentration", "comparator": "Matched culture without formulation", "endpoint": "Pure glyphosate did not inhibit growth under the cited GT+-sensitive test conditions.", "effect_direction": "no_detected_change", "combination": "single", "conditions": []} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Bacterial culture comparison separate from the rat study.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Specific formulation and concentration comparison, not universal absence or presence of inhibition.
    plain_language
    Pure glyphosate did not inhibit growth under the cited GT+-sensitive test conditions.
    primary_references
    Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/
    source_locator
    Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.

    Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 39–39

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Bacterial culture comparison separate from the rat study. · source_derived_draft · unverified_draft

    **Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)
    Complete structured claim and evidence

  2. GT+ reduced L. rhamnosus growth in the tested comparison.

    Roundup GT+, tested glyphosate formulation → Lactobacillus rhamnosus growth in formulation-comparison assays source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.
    experimental_contrast
    {"intervention": "roundup-gt-plus at the reported tested concentration", "comparator": "Matched culture without formulation", "endpoint": "GT+ reduced L. rhamnosus growth in the tested comparison.", "effect_direction": "decrease", "combination": "single", "conditions": []} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Bacterial culture comparison separate from the rat study.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Specific formulation and concentration comparison, not universal absence or presence of inhibition.
    plain_language
    GT+ reduced L. rhamnosus growth in the tested comparison.
    primary_references
    Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/
    source_locator
    Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.

    Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 39–39

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Bacterial culture comparison separate from the rat study. · source_derived_draft · unverified_draft

    **Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)
    Complete structured claim and evidence

  3. MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.

    Roundup MON52276, tested glyphosate formulation → Lactobacillus rhamnosus growth in formulation-comparison assays source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.
    experimental_contrast
    {"intervention": "roundup-mon52276 at the reported tested concentration", "comparator": "Matched culture without formulation", "endpoint": "MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.", "effect_direction": "no_detected_change", "combination": "single", "conditions": []} Explicit extracted experimental comparison; source-derived draft.
    experimental_model
    Bacterial culture comparison separate from the rat study.
    interpretation_status
    Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.
    limitations
    Specific formulation and concentration comparison, not universal absence or presence of inhibition.
    plain_language
    MON52276 did not inhibit growth under the cited GT+-sensitive test conditions.
    primary_references
    Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/
    source_locator
    Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.

    Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026) · lines 39–39

    Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication. · supports · Bacterial culture comparison separate from the rat study. · source_derived_draft · unverified_draft

    **Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.