{"id":"1ab47545-6866-517c-b911-440f9158449c","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:rat-cecal-gamma-glutamylglutamine","predicate":"increases_in_recorded_experiment","statement":"The rat glyphosate/formulation study recorded increased cecal gamma-glutamylglutamine.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6c3a2bce-0abe-5875-ad5a-3fe9fba55c11","mechanism_event_label":"The rat glyphosate/formulation study recorded increased cecal gamma-glutamylglutamine.","subject":{"id":"aad546fc-b049-5f3c-b91a-2c5458873f29","slug":"glyphosate","display_name":"Glyphosate","entity_type_key":"small_molecule"},"object":{"id":"c6a2c5d1-d821-50d0-96e3-1b4fe6abdea3","slug":"rat-cecal-gamma-glutamylglutamine","display_name":"Rat cecal gamma-glutamylglutamine pool","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"6c3a2bce-0abe-5875-ad5a-3fe9fba55c11","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:rat-cecal-gamma-glutamylglutamine-event","event_type":"experimental_observation","label":"The rat glyphosate/formulation study recorded increased cecal gamma-glutamylglutamine.","description":"**Microbial metabolites do not automatically establish host deficiency.** The same rat study recorded cecal changes in cysteinylglycine, gamma-glutamylglutamine and valylglycine, among other metabolites. 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[Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aad546fc-b049-5f3c-b91a-2c5458873f29","slug":"glyphosate","display_name":"Glyphosate","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"c6a2c5d1-d821-50d0-96e3-1b4fe6abdea3","slug":"rat-cecal-gamma-glutamylglutamine","display_name":"Rat cecal gamma-glutamylglutamine pool","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"60ab6ff2-50fa-5f69-b092-9bd293a65a64","slug":"roundup-mon52276","display_name":"Roundup MON52276, tested glyphosate formulation","entity_type_key":"chemical_species"},"role":"other studied treatment","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. 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These pools are not direct measurements of host glutathione synthesis, nutrient sufficiency or pathway flux. In separate bacterial culture comparisons, Roundup GT+ suppressed L. rhamnosus growth under conditions where pure glyphosate and MON52276 did not. Formulations therefore retain separate actor identities. A dose in mg/kg/day cannot be converted into a microbial mM exposure without measured concentration, distribution and time. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","model_system":"90-day rat metabolomics; study-level observed pool change.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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