{"id":"e7554727-ce03-5444-8bd8-3f2325add8b6","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:alpha-diversity-null","predicate":"no_detected_change_in_recorded_experiment","statement":"Overall alpha-diversity differences in the study were not significant (p=0.09).","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"770ac0e2-8d7d-57b6-9d26-4c536b638b52","mechanism_event_label":"Overall alpha-diversity differences in the study were not significant (p=0.09).","subject":{"id":"aad546fc-b049-5f3c-b91a-2c5458873f29","slug":"glyphosate","display_name":"Glyphosate","entity_type_key":"small_molecule"},"object":{"id":"edfd8433-0f75-54f8-9ece-748c205150f6","slug":"rat-microbiome-alpha-diversity","display_name":"Rat microbiome alpha diversity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"770ac0e2-8d7d-57b6-9d26-4c536b638b52","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:alpha-diversity-null-event","event_type":"experimental_observation","label":"Overall alpha-diversity differences in the study were not significant (p=0.09).","description":"**An upstream pool can rise when flux is blocked.** Mesnage 2021 compared pure glyphosate and Roundup MON52276 in female Sprague–Dawley rats for 90 days at 0.5, 50 and 175 mg/kg/day glyphosate-equivalent exposures, with 12 animals per group. Cecal shikimate and DHS accumulation supported pathway inhibition; abundance alone was less informative. The collection records study-level accumulation rather than asserting every dose had an identical significant effect. Overall alpha-diversity differences were not significant (p=0.09), while beta diversity differed. Selected low-abundance taxa changed, and formulation-specific differences remained. Serum shikimate was undetected with the employed method, whose sensitivity and protein interference limit a zero-exposure interpretation. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aad546fc-b049-5f3c-b91a-2c5458873f29","slug":"glyphosate","display_name":"Glyphosate","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"edfd8433-0f75-54f8-9ece-748c205150f6","slug":"rat-microbiome-alpha-diversity","display_name":"Rat microbiome alpha diversity","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""},{"entity":{"id":"60ab6ff2-50fa-5f69-b092-9bd293a65a64","slug":"roundup-mon52276","display_name":"Roundup MON52276, tested glyphosate formulation","entity_type_key":"chemical_species"},"role":"other tested actor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Across glyphosate and MON52276 treatment groups in the 90-day rat study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Diversity null does not erase metabolomic differences or establish absence of biological effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Overall alpha-diversity differences in the study were not significant (p=0.09).","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats. | 2021 | DOI 10.1289/ehp6990 | PMID 33502259 | https://pubmed.ncbi.nlm.nih.gov/33502259/ | https://doi.org/10.1289/ehp6990 | https://pmc.ncbi.nlm.nih.gov/articles/PMC7839352/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 37-37; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"880bbc05-98c4-5107-985b-300313c1f14a","evidence_kind":"source_excerpt","locator":"Lines 37-37","start_line":37,"end_line":37,"excerpt":"**An upstream pool can rise when flux is blocked.** Mesnage 2021 compared pure glyphosate and Roundup MON52276 in female Sprague–Dawley rats for 90 days at 0.5, 50 and 175 mg/kg/day glyphosate-equivalent exposures, with 12 animals per group. Cecal shikimate and DHS accumulation supported pathway inhibition; abundance alone was less informative. The collection records study-level accumulation rather than asserting every dose had an identical significant effect. Overall alpha-diversity differences were not significant (p=0.09), while beta diversity differed. Selected low-abundance taxa changed, and formulation-specific differences remained. Serum shikimate was undetected with the employed method, whose sensitivity and protein interference limit a zero-exposure interpretation. [Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats.](https://pubmed.ncbi.nlm.nih.gov/33502259/)","model_system":"Across glyphosate and MON52276 treatment groups in the 90-day rat study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}