Component

Rat myotube glucose uptake

Rat myotube glucose uptake. Species, exposure and limitations are retained in each linked claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. AMPK alpha1/alpha2 knockdown abrogated CGA-stimulated glucose transport.

    Experimental rat AMPK alpha1/alpha2 knockdown → Rat myotube glucose uptake source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_span
    {"source_cache": "artifacts/chlorogenic_acid-research/22412912.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff", "start_char": 0, "end_char": 1896, "text_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff"}
    experimental_model
    L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments
    exposure
    L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally
    limitations
    Millimolar culture exposure and injected mouse doses are not dietary human exposures. Phosphorylation and abundance readouts do not by themselves prove every upstream causal step.
    nutrient_topic
    Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid
    organism
    Rattus norvegicus
    plain_language
    The response depends on functioning machinery in this model.
    primary_references
    [chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. (2012). https://pubmed.ncbi.nlm.nih.gov/22412912/ DOI: 10.1371/journal.pone.0032718
    tissue_or_cell_type
    L6 myotubes
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 490–501

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments · source_derived_draft · unverified_draft

    ### chlorogenic_acid-ampk-loss AMPK alpha1/alpha2 knockdown abrogated CGA-stimulated glucose transport. Condition category: machinery_impairment nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: The response depends on functioning machinery in this model. organism: Rattus norvegicus tissue_or_cell_type: L6 myotubes experimental_model: L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments limitations: Millimolar culture exposure and injected mouse doses are not dietary human exposures. Phosphorylation and abundance readouts do not by themselves prove every upstream causal step. exposure: L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/22412912.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff", "start_char": 0, "end_char": 1896, "text_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff"} [chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. (2012). https://pubmed.ncbi.nlm.nih.gov/22412912/ DOI: 10.1371/journal.pone.0032718
    Complete structured claim and evidence

  2. L6 2-deoxyglucose uptake increased with CGA concentration and exposure time.

    Chlorogenic acid / 5-O-caffeoylquinic acid → Rat myotube glucose uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/chlorogenic_acid-research/22412912.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff", "start_char": 0, "end_char": 1896, "text_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff"}
    experimental_model
    L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments
    exposure
    L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally
    limitations
    Millimolar culture exposure and injected mouse doses are not dietary human exposures. Phosphorylation and abundance readouts do not by themselves prove every upstream causal step.
    nutrient_topic
    Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. · Chlorogenic acid / 5-O-caffeoylquinic acid
    organism
    Rattus norvegicus
    plain_language
    This measures cell glucose handling, not diabetes remission.
    primary_references
    [chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. (2012). https://pubmed.ncbi.nlm.nih.gov/22412912/ DOI: 10.1371/journal.pone.0032718
    tissue_or_cell_type
    L6 skeletal-muscle myotubes

    Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17) · lines 451–462

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments · source_derived_draft · unverified_draft

    ### chlorogenic_acid-glucose-uptake L6 2-deoxyglucose uptake increased with CGA concentration and exposure time. Condition category: normal nutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: This measures cell glucose handling, not diabetes remission. organism: Rattus norvegicus tissue_or_cell_type: L6 skeletal-muscle myotubes experimental_model: L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments limitations: Millimolar culture exposure and injected mouse doses are not dietary human exposures. Phosphorylation and abundance readouts do not by themselves prove every upstream causal step. exposure: L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally evidence_span: {"source_cache": "artifacts/chlorogenic_acid-research/22412912.abstract.txt", "locator": "Primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff", "start_char": 0, "end_char": 1896, "text_sha256": "77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff"} [chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. (2012). https://pubmed.ncbi.nlm.nih.gov/22412912/ DOI: 10.1371/journal.pone.0032718
    Complete structured claim and evidence

  3. p38 inhibitors reduced lipoic-acid-stimulated glucose uptake without blocking GLUT4 translocation in L6 myotubes.

    Rat p38 MAP kinase enzymes → Rat myotube glucose uptake source_derived_draftungraded
    Experimental context and source evidence
    evidence_span
    {"source_cache": "artifacts/ala-research/11375349.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "971e4224c0ff09f05d25fe64359a3c65d50f2187fd781840c55821c47d2ed111", "start_char": 0, "end_char": 1734, "text_sha256": "971e4224c0ff09f05d25fe64359a3c65d50f2187fd781840c55821c47d2ed111"}
    experimental_model
    Signaling, transporter localization and glucose-uptake assays
    exposure
    2.5 mmol/L lipoic acid; PI3K and p38 inhibitors
    limitations
    High cell-culture exposure; pharmacological inhibitors can have off-target effects; not a plasma target or patient dose.
    nutrient_topic
    Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect. · Lipoic acid
    organism
    Rat
    plain_language
    Transporter location and actual glucose movement were separable measurements.
    primary_references
    [ala-p11375349] The antihyperglycemic drug alpha-lipoic acid stimulates glucose uptake via both GLUT4 translocation and GLUT4 activation: potential role of p38 mitogen-activated protein kinase in GLUT4 activation. (2001). https://pubmed.ncbi.nlm.nih.gov/11375349/ DOI: 10.2337/diabetes.50.6.1464
    tissue_or_cell_type
    L6 myotubes

    Alpha-lipoic acid: cofactor assembly, redox signaling and nutrient interactions (2026-09-17) · lines 923–934

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Signaling, transporter localization and glucose-uptake assays · source_derived_draft · unverified_draft

    ### ala-p38-uptake-localization p38 inhibitors reduced lipoic-acid-stimulated glucose uptake without blocking GLUT4 translocation in L6 myotubes. Condition category: normal nutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect. plain_language: Transporter location and actual glucose movement were separable measurements. organism: Rat tissue_or_cell_type: L6 myotubes experimental_model: Signaling, transporter localization and glucose-uptake assays limitations: High cell-culture exposure; pharmacological inhibitors can have off-target effects; not a plasma target or patient dose. exposure: 2.5 mmol/L lipoic acid; PI3K and p38 inhibitors evidence_span: {"source_cache": "artifacts/ala-research/11375349.abstract.txt", "locator": "Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets", "file_sha256": "971e4224c0ff09f05d25fe64359a3c65d50f2187fd781840c55821c47d2ed111", "start_char": 0, "end_char": 1734, "text_sha256": "971e4224c0ff09f05d25fe64359a3c65d50f2187fd781840c55821c47d2ed111"} [ala-p11375349] The antihyperglycemic drug alpha-lipoic acid stimulates glucose uptake via both GLUT4 translocation and GLUT4 activation: potential role of p38 mitogen-activated protein kinase in GLUT4 activation. (2001). https://pubmed.ncbi.nlm.nih.gov/11375349/ DOI: 10.2337/diabetes.50.6.1464
    Complete structured claim and evidence

  4. Adding (S)-6-gingerol increased glucose uptake in rat L6 myotubes.

    6-Gingerol → Rat myotube glucose uptake source_derived_draftungraded
    Experimental context and source evidence
    dose
    (S)-6-Gingerol; dose-response, exact range not in accessed abstract
    duration
    Time-dependent response; exact times not in accessed abstract
    evidence_access
    Primary PubMed abstract.
    evidence_scope
    literature_reviewed; model-specific source-derived curation, not universally established human effects
    experimental_model
    Rat L6 skeletal muscle myotubes
    limitations
    Calcium/CaMKK-associated signaling does not demonstrate dietary calcium depletion or calcium-supplement synergy; no human glycemic outcome was tested.
    nutrient_topic
    Gingerols collection; each molecular form and experimental preparation remains explicit. · Gingerols
    organism
    Rat L6 skeletal muscle myotubes
    plain_language
    Adding (S)-6-gingerol increased glucose uptake in rat L6 myotubes.
    primary_references
    (S)-[6]-Gingerol enhances glucose uptake in L6 myotubes by activation of AMPK in response to [Ca2+]i. (2013). https://pubmed.ncbi.nlm.nih.gov/23958199/ DOI: 10.18433/j34g7p
    route
    In vitro addition
    tissue
    Skeletal muscle cell model

    Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 173–182

    Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text. · supports · Rat L6 skeletal muscle myotubes · source_derived_draft · unverified_draft

    ## gingerols-6-l6-glucose Adding (S)-6-gingerol increased glucose uptake in rat L6 myotubes. Model/species: Rat L6 skeletal muscle myotubes Tissue: Skeletal muscle cell model Exposure: (S)-6-Gingerol; dose-response, exact range not in accessed abstract Route: In vitro addition Duration: Time-dependent response; exact times not in accessed abstract Limits: Calcium/CaMKK-associated signaling does not demonstrate dietary calcium depletion or calcium-supplement synergy; no human glycemic outcome was tested. Primary reference: (S)-[6]-Gingerol enhances glucose uptake in L6 myotubes by activation of AMPK in response to [Ca2+]i. (2013). https://pubmed.ncbi.nlm.nih.gov/23958199/ DOI: 10.18433/j34g7p Access: Primary PubMed abstract.
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

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