{"id":"4f0b3e52-e7b5-5870-b19e-36f0c4cb0079","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-ampk-loss","predicate":"abrogates","statement":"AMPK alpha1/alpha2 knockdown abrogated CGA-stimulated glucose transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"27af5acc-65e1-5e09-8d6b-781b2bfd0b31","mechanism_event_label":"The response depends on functioning machinery in this model.","subject":{"id":"167398aa-6d49-5b08-99b8-b0ed1b2a52b1","slug":"rat-ampk-alpha-knockdown","display_name":"Experimental rat AMPK alpha1/alpha2 knockdown","entity_type_key":"protein_state"},"object":{"id":"b6ecad8e-71de-5e96-a89e-aba452579d4e","slug":"rat-myotube-glucose-uptake","display_name":"Rat myotube glucose uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"27af5acc-65e1-5e09-8d6b-781b2bfd0b31","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-ampk-loss-event","event_type":"biochemical_relationship","label":"The response depends on functioning machinery in this model.","description":"AMPK alpha1/alpha2 knockdown abrogated CGA-stimulated glucose transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"affected_machinery","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"role":"tested_stimulus","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"167398aa-6d49-5b08-99b8-b0ed1b2a52b1","slug":"rat-ampk-alpha-knockdown","display_name":"Experimental rat AMPK alpha1/alpha2 knockdown","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b6ecad8e-71de-5e96-a89e-aba452579d4e","slug":"rat-myotube-glucose-uptake","display_name":"Rat myotube glucose uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chlorogenic_acid-research/22412912.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\", \"start_char\": 0, \"end_char\": 1896, \"text_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar culture exposure and injected mouse doses are not dietary human exposures. 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Phosphorylation and abundance readouts do not by themselves prove every upstream causal step.\nexposure: L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally\nevidence_span: {\"source_cache\": \"artifacts/chlorogenic_acid-research/22412912.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\", \"start_char\": 0, \"end_char\": 1896, \"text_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\"}\n[chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. 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