Component

Human 5-HT3 receptor preparation in HEK293 membranes

Human receptor used for GR65630 competition binding; exact subunit composition not specified in the accessed abstract.

3 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. 10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.

    10-Gingerol → Human 5-HT3 receptor preparation in HEK293 membranes source_derived_draftungraded
    Experimental context and source evidence
    dose
    Gingerols with tritiated GR65630; exact concentration range not in accessed abstract
    duration
    Equilibrium assay; duration not specified in accessed abstract
    evidence_access
    Primary PubMed abstract.
    evidence_scope
    literature_reviewed; model-specific source-derived curation, not universally established human effects
    experimental_model
    Human 5-HT3 receptors in HEK293 membrane preparations
    limitations
    Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site.
    nutrient_topic
    Gingerols collection; each molecular form and experimental preparation remains explicit. · Gingerols
    organism
    Human 5-HT3 receptors in HEK293 membrane preparations
    plain_language
    10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.
    primary_references
    Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049
    route
    In vitro competition binding
    tissue
    Receptor preparation

    Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 129–138

    Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text. · supports · Human 5-HT3 receptors in HEK293 membrane preparations · source_derived_draft · unverified_draft

    ## gingerols-10-5ht3-binding-null 10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation. Model/species: Human 5-HT3 receptors in HEK293 membrane preparations Tissue: Receptor preparation Exposure: Gingerols with tritiated GR65630; exact concentration range not in accessed abstract Route: In vitro competition binding Duration: Equilibrium assay; duration not specified in accessed abstract Limits: Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site. Primary reference: Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049 Access: Primary PubMed abstract.
    Complete structured claim and evidence

  2. 6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.

    6-Gingerol → Human 5-HT3 receptor preparation in HEK293 membranes source_derived_draftungraded
    Experimental context and source evidence
    dose
    Gingerols with tritiated GR65630; exact concentration range not in accessed abstract
    duration
    Equilibrium assay; duration not specified in accessed abstract
    evidence_access
    Primary PubMed abstract.
    evidence_scope
    literature_reviewed; model-specific source-derived curation, not universally established human effects
    experimental_model
    Human 5-HT3 receptors in HEK293 membrane preparations
    limitations
    Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site.
    nutrient_topic
    Gingerols collection; each molecular form and experimental preparation remains explicit. · Gingerols
    organism
    Human 5-HT3 receptors in HEK293 membrane preparations
    plain_language
    6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.
    primary_references
    Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049
    route
    In vitro competition binding
    tissue
    Receptor preparation

    Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 63–72

    Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text. · supports · Human 5-HT3 receptors in HEK293 membrane preparations · source_derived_draft · unverified_draft

    ## gingerols-6-5ht3-binding-null 6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation. Model/species: Human 5-HT3 receptors in HEK293 membrane preparations Tissue: Receptor preparation Exposure: Gingerols with tritiated GR65630; exact concentration range not in accessed abstract Route: In vitro competition binding Duration: Equilibrium assay; duration not specified in accessed abstract Limits: Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site. Primary reference: Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049 Access: Primary PubMed abstract.
    Complete structured claim and evidence

  3. 8-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.

    8-Gingerol → Human 5-HT3 receptor preparation in HEK293 membranes source_derived_draftungraded
    Experimental context and source evidence
    dose
    Gingerols with tritiated GR65630; exact concentration range not in accessed abstract
    duration
    Equilibrium assay; duration not specified in accessed abstract
    evidence_access
    Primary PubMed abstract.
    evidence_scope
    literature_reviewed; model-specific source-derived curation, not universally established human effects
    experimental_model
    Human 5-HT3 receptors in HEK293 membrane preparations
    limitations
    Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site.
    nutrient_topic
    Gingerols collection; each molecular form and experimental preparation remains explicit. · Gingerols
    organism
    Human 5-HT3 receptors in HEK293 membrane preparations
    plain_language
    8-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.
    primary_references
    Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049
    route
    In vitro competition binding
    tissue
    Receptor preparation

    Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20) · lines 96–105

    Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text. · supports · Human 5-HT3 receptors in HEK293 membrane preparations · source_derived_draft · unverified_draft

    ## gingerols-8-5ht3-binding-null 8-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation. Model/species: Human 5-HT3 receptors in HEK293 membrane preparations Tissue: Receptor preparation Exposure: Gingerols with tritiated GR65630; exact concentration range not in accessed abstract Route: In vitro competition binding Duration: Equilibrium assay; duration not specified in accessed abstract Limits: Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site. Primary reference: Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049 Access: Primary PubMed abstract.
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards