{"id":"e15a6b56-2980-50bc-96c4-b02467e9e391","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-10-5ht3-binding-null","predicate":"does_not_displace_radioligand","statement":"10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"d75fe27f-dcb9-58f9-92de-c42d28fad5fe","mechanism_event_label":"10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","subject":{"id":"bf4fa054-0b02-5fc2-82dd-2e53b36033b3","slug":"10-gingerol","display_name":"10-Gingerol","entity_type_key":"small_molecule"},"object":{"id":"a0743cdf-d2cc-5b95-baf4-0c4ea5d462f8","slug":"human-5ht3-receptor-preparation","display_name":"Human 5-HT3 receptor preparation in HEK293 membranes","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"d75fe27f-dcb9-58f9-92de-c42d28fad5fe","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-10-5ht3-binding-null-event","event_type":"observed_relationship","label":"10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","description":"10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bf4fa054-0b02-5fc2-82dd-2e53b36033b3","slug":"10-gingerol","display_name":"10-Gingerol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a0743cdf-d2cc-5b95-baf4-0c4ea5d462f8","slug":"human-5ht3-receptor-preparation","display_name":"Human 5-HT3 receptor preparation in HEK293 membranes","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Gingerols with tritiated GR65630; exact concentration range not in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Equilibrium assay; duration not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human 5-HT3 receptors in HEK293 membrane preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human 5-HT3 receptors in HEK293 membrane preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"10-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. 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