{"id":"6835051d-6f33-5378-97b0-2a5420f381f8","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-5ht3-binding-null","predicate":"does_not_displace_radioligand","statement":"6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"7614c9e1-f1e1-55dc-9811-ae3168138373","mechanism_event_label":"6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","subject":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"object":{"id":"a0743cdf-d2cc-5b95-baf4-0c4ea5d462f8","slug":"human-5ht3-receptor-preparation","display_name":"Human 5-HT3 receptor preparation in HEK293 membranes","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"7614c9e1-f1e1-55dc-9811-ae3168138373","stable_key":"fd7915ff-856d-56c2-a118-4989693a7644:gingerols-6-5ht3-binding-null-event","event_type":"observed_relationship","label":"6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","description":"6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"837fdd65-c1eb-52e0-a724-42a1e9065c82","slug":"6-gingerol","display_name":"6-Gingerol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a0743cdf-d2cc-5b95-baf4-0c4ea5d462f8","slug":"human-5ht3-receptor-preparation","display_name":"Human 5-HT3 receptor preparation in HEK293 membranes","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Gingerols with tritiated GR65630; exact concentration range not in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Equilibrium assay; duration not specified in accessed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation, not universally established human effects","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human 5-HT3 receptors in HEK293 membrane preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gingerols collection; each molecular form and experimental preparation remains explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gingerols","display_name":"Gingerols","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human 5-HT3 receptors in HEK293 membrane preparations","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro competition binding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Receptor preparation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9a9430f6-fad1-56a8-b7ff-dbf5ff2c18c2","evidence_kind":"source_excerpt","locator":"Lines 63-72","start_line":63,"end_line":72,"excerpt":"## gingerols-6-5ht3-binding-null\n6-Gingerol did not displace GR65630 from the human 5-HT3 receptor preparation.\nModel/species: Human 5-HT3 receptors in HEK293 membrane preparations\nTissue: Receptor preparation\nExposure: Gingerols with tritiated GR65630; exact concentration range not in accessed abstract\nRoute: In vitro competition binding\nDuration: Equilibrium assay; duration not specified in accessed abstract\nLimits: Failure to displace this radioligand supports a distinct mechanism but does not locate an allosteric site.\nPrimary reference: Mode of action of gingerols and shogaols on 5-HT3 receptors: binding studies, cation uptake by the receptor channel and contraction of isolated guinea-pig ileum. (2006). https://pubmed.ncbi.nlm.nih.gov/16364290/ DOI: 10.1016/j.ejphar.2005.10.049\nAccess: Primary PubMed abstract.","model_system":"Human 5-HT3 receptors in HEK293 membrane preparations","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived synthesis span with its primary citation; original paraphrase, not a claimed publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1107d60b-8e2d-55d7-8f5d-5612c49aa7dc","stable_key":"import-fd7915ff-856d-56c2-a118-4989693a7644","title":"Gingerols: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen primary research papers; study-specific PubMed/DOI links and limitations retained. Not publisher full text.","file_path":"","sha256":"96d1b7b04445eecd0eac1738e0c80cb6308509ecdfc3b5fc6be67ae2218cbb2b","revision_id":"de6e70ff-0e52-5c26-bd3a-54fca2085a86","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}