Component

Experimental arginine-depleted culture medium

Study-scoped entity; inspect species, exposure and experimental limitations on each claim.

5 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. Arginine removal lowered CD3-zeta and proliferation; replacing arginine reversed the changes.

    Experimental arginine-depleted culture medium → Human T-cell receptor CD3-zeta chain / CD247 source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Jurkat T cells in arginine-free culture medium.
    limitations
    Not a clinical dietary-deficiency threshold.
    nutrient_topic
    L-Arginine collection; tissue, species, dose and formulation distinctions retained. · L-Arginine
    plain_language
    The receptor’s signaling machinery could recover when arginine returned.
    primary_references
    Regulation of T cell receptor CD3zeta chain expression by L-arginine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11950832/ · DOI 10.1074/jbc.m110675200
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18) · lines 278–284

    AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Jurkat T cells in arginine-free culture medium. · source_derived_draft · unverified_draft

    ## arg-jurkat-protein The receptor’s signaling machinery could recover when arginine returned. Arginine removal lowered CD3-zeta and proliferation; replacing arginine reversed the changes. Model: Jurkat T cells in arginine-free culture medium. Limitations: Not a clinical dietary-deficiency threshold. Evidence access: Primary abstract Regulation of T cell receptor CD3zeta chain expression by L-arginine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11950832/ · DOI 10.1074/jbc.m110675200
    Complete structured claim and evidence

  2. Arginine removal shortened CD247 mRNA half-life without reducing its transcription rate.

    Experimental arginine-depleted culture medium → Human CD247 messenger RNA source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Jurkat T cells in arginine-free culture medium.
    limitations
    Cell-line result; primary T cells showed a different regulatory level in the later study.
    nutrient_topic
    L-Arginine collection; tissue, species, dose and formulation distinctions retained. · L-Arginine
    plain_language
    Low availability changed how long an immune-signaling message survived.
    primary_references
    Regulation of T cell receptor CD3zeta chain expression by L-arginine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11950832/ · DOI 10.1074/jbc.m110675200
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18) · lines 270–276

    AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Jurkat T cells in arginine-free culture medium. · source_derived_draft · unverified_draft

    ## arg-jurkat-rna Low availability changed how long an immune-signaling message survived. Arginine removal shortened CD247 mRNA half-life without reducing its transcription rate. Model: Jurkat T cells in arginine-free culture medium. Limitations: Cell-line result; primary T cells showed a different regulatory level in the later study. Evidence access: Primary abstract Regulation of T cell receptor CD3zeta chain expression by L-arginine. · 2002 · https://pubmed.ncbi.nlm.nih.gov/11950832/ · DOI 10.1074/jbc.m110675200
    Complete structured claim and evidence

  3. Activated human T cells failed to restore CD3-zeta in arginine-free medium; replenishment restored expression.

    Experimental arginine-depleted culture medium → Human T-cell receptor CD3-zeta chain / CD247 source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Activated human T-lymphocyte culture.
    limitations
    Unlike Jurkat findings, lower mRNA, greater degradation and apoptosis did not explain this result. Model difference is retained.
    nutrient_topic
    L-Arginine collection; tissue, species, dose and formulation distinctions retained. · L-Arginine
    plain_language
    Availability affected rebuilding the receptor after stimulation.
    primary_references
    L-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18) · lines 286–292

    AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Activated human T-lymphocyte culture. · source_derived_draft · unverified_draft

    ## arg-primary-cd3 Availability affected rebuilding the receptor after stimulation. Activated human T cells failed to restore CD3-zeta in arginine-free medium; replenishment restored expression. Model: Activated human T-lymphocyte culture. Limitations: Unlike Jurkat findings, lower mRNA, greater degradation and apoptosis did not explain this result. Model difference is retained. Evidence access: Primary abstract L-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004
    Complete structured claim and evidence

  4. IFN-gamma, IL-5 and IL-10 output decreased, whereas IL-2 did not show the same decrease.

    Experimental arginine-depleted culture medium → Activated T-cell cytokine output source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Activated human T-lymphocyte culture.
    limitations
    Not evidence that all cytokines or all immune functions decline together.
    nutrient_topic
    L-Arginine collection; tissue, species, dose and formulation distinctions retained. · L-Arginine
    plain_language
    The immune response changed selectively.
    primary_references
    L-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18) · lines 294–300

    AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Activated human T-lymphocyte culture. · source_derived_draft · unverified_draft

    ## arg-primary-cytokines The immune response changed selectively. IFN-gamma, IL-5 and IL-10 output decreased, whereas IL-2 did not show the same decrease. Model: Activated human T-lymphocyte culture. Limitations: Not evidence that all cytokines or all immune functions decline together. Evidence access: Primary abstract L-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. CASTOR1 was required for arginine deprivation to inhibit mTORC1.

    Human cellular arginine sensor CASTOR1 → Mechanistic target of rapamycin complex 1 source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    nutrient_deficiency Imported condition classification; unverified.
    evidence_access
    Primary abstract
    experimental_model
    Mammalian-cell signaling experiments and biochemical CASTOR1 binding.
    limitations
    Experimental starvation response; no universal dietary cutoff.
    nutrient_topic
    L-Arginine collection; tissue, species, dose and formulation distinctions retained. · L-Arginine
    plain_language
    Low arginine is sensed through specific machinery.
    primary_references
    The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26972053/ · DOI 10.1016/j.cell.2016.02.035
    trigger_kind
    nutrient_deficiency Imported condition classification; unverified.

    L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18) · lines 190–196

    AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text. · supports · Mammalian-cell signaling experiments and biochemical CASTOR1 binding. · source_derived_draft · unverified_draft

    ## arg-castor-starvation Low arginine is sensed through specific machinery. CASTOR1 was required for arginine deprivation to inhibit mTORC1. Model: Mammalian-cell signaling experiments and biochemical CASTOR1 binding. Limitations: Experimental starvation response; no universal dietary cutoff. Evidence access: Primary abstract The CASTOR Proteins Are Arginine Sensors for the mTORC1 Pathway. · 2016 · https://pubmed.ncbi.nlm.nih.gov/26972053/ · DOI 10.1016/j.cell.2016.02.035
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards