{"id":"b0fd7f23-997f-5f00-8f50-0a7eed0189c1","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-primary-cytokines","predicate":"reduces","statement":"IFN-gamma, IL-5 and IL-10 output decreased, whereas IL-2 did not show the same decrease.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"7dfa25a5-3394-5bd3-985c-bed8bc68a946","mechanism_event_label":"The immune response changed selectively.","subject":{"id":"9bc75d40-8d7f-5314-aaf3-2dbf08db974c","slug":"arginine-depleted-medium","display_name":"Experimental arginine-depleted culture medium","entity_type_key":"cellular_process"},"object":{"id":"efbad9c9-9262-5845-9dac-038fdadd6db0","slug":"t-cell-cytokine-output","display_name":"Activated T-cell cytokine output","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7dfa25a5-3394-5bd3-985c-bed8bc68a946","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-primary-cytokines-event","event_type":"observed_relationship","label":"The immune response changed selectively.","description":"IFN-gamma, IL-5 and IL-10 output decreased, whereas IL-2 did not show the same decrease.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9bc75d40-8d7f-5314-aaf3-2dbf08db974c","slug":"arginine-depleted-medium","display_name":"Experimental arginine-depleted culture medium","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"efbad9c9-9262-5845-9dac-038fdadd6db0","slug":"t-cell-cytokine-output","display_name":"Activated T-cell cytokine output","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Activated human T-lymphocyte culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence that all cytokines or all immune functions decline together.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The immune response changed selectively.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"L-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"15a9cd6e-5289-5f2f-9f86-61af143a8326","evidence_kind":"source_excerpt","locator":"Lines 294-300","start_line":294,"end_line":300,"excerpt":"## arg-primary-cytokines\nThe immune response changed selectively.\nIFN-gamma, IL-5 and IL-10 output decreased, whereas IL-2 did not show the same decrease.\nModel: Activated human T-lymphocyte culture.\nLimitations: Not evidence that all cytokines or all immune functions decline together.\nEvidence access: Primary abstract\nL-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004","model_system":"Activated human T-lymphocyte culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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