{"id":"b32ac52e-ebb1-57e0-9ed8-2b0361090bb5","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-primary-cd3","predicate":"impairs_reexpression","statement":"Activated human T cells failed to restore CD3-zeta in arginine-free medium; replenishment restored expression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"2ea5cf0e-f15b-5a19-9c0b-a684720aef44","mechanism_event_label":"Availability affected rebuilding the receptor after stimulation.","subject":{"id":"9bc75d40-8d7f-5314-aaf3-2dbf08db974c","slug":"arginine-depleted-medium","display_name":"Experimental arginine-depleted culture medium","entity_type_key":"cellular_process"},"object":{"id":"585aab16-4259-5661-bd66-9345952f487f","slug":"cd247","display_name":"Human T-cell receptor CD3-zeta chain / CD247","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2ea5cf0e-f15b-5a19-9c0b-a684720aef44","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-primary-cd3-event","event_type":"observed_relationship","label":"Availability affected rebuilding the receptor after stimulation.","description":"Activated human T cells failed to restore CD3-zeta in arginine-free medium; replenishment restored expression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9bc75d40-8d7f-5314-aaf3-2dbf08db974c","slug":"arginine-depleted-medium","display_name":"Experimental arginine-depleted culture medium","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"585aab16-4259-5661-bd66-9345952f487f","slug":"cd247","display_name":"Human T-cell receptor CD3-zeta chain / CD247","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Activated human T-lymphocyte culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Unlike Jurkat findings, lower mRNA, greater degradation and apoptosis did not explain this result. Model difference is retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Availability affected rebuilding the receptor after stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"L-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"26cfcaf7-8bdf-52ca-a3ce-ebd287130760","evidence_kind":"source_excerpt","locator":"Lines 286-292","start_line":286,"end_line":292,"excerpt":"## arg-primary-cd3\nAvailability affected rebuilding the receptor after stimulation.\nActivated human T cells failed to restore CD3-zeta in arginine-free medium; replenishment restored expression.\nModel: Activated human T-lymphocyte culture.\nLimitations: Unlike Jurkat findings, lower mRNA, greater degradation and apoptosis did not explain this result. Model difference is retained.\nEvidence access: Primary abstract\nL-Arginine modulates CD3zeta expression and T cell function in activated human T lymphocytes. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15922712/ · DOI 10.1016/j.cellimm.2005.01.004","model_system":"Activated human T-lymphocyte culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"ec0e9a3fb72b7485534e4372a074ac7624a399ff962e55690eb462217b5149ec","revision_id":"af027270-5535-51ae-85ff-9c76c518c962","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}