{"id":"da297e13-c8b6-5cb1-9d6b-27a8f667d857","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-jurkat-protein","predicate":"reduces","statement":"Arginine removal lowered CD3-zeta and proliferation; replacing arginine reversed the changes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a908e75e-be88-53d1-8411-55071a2170f1","mechanism_event_label":"The receptor’s signaling machinery could recover when arginine returned.","subject":{"id":"9bc75d40-8d7f-5314-aaf3-2dbf08db974c","slug":"arginine-depleted-medium","display_name":"Experimental arginine-depleted culture medium","entity_type_key":"cellular_process"},"object":{"id":"585aab16-4259-5661-bd66-9345952f487f","slug":"cd247","display_name":"Human T-cell receptor CD3-zeta chain / CD247","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"a908e75e-be88-53d1-8411-55071a2170f1","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-jurkat-protein-event","event_type":"observed_relationship","label":"The receptor’s signaling machinery could recover when arginine returned.","description":"Arginine removal lowered CD3-zeta and proliferation; 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primary-abstract references and experimental limitations individually identified. 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