Component

TRPM5 channel in the 2017 expression assay, construct species not independently verified

Context-specific entity; species, compartment and exposure are stated on each claim.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What acts on it

  1. Buffering intracellular free calcium prevented stevioside from activating TRPM5 in the expression assay.

    Calcium ion → TRPM5 channel in the 2017 expression assay, construct species not independently verified source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    evidence_access
    Primary full text; Figure 1d and methods
    experimental_model
    Intracellular calcium buffered with BAPTA; whole-cell recordings.
    limitations
    This is a local signaling requirement, not evidence of dietary calcium deficiency or benefit from a calcium supplement.
    nutrient_topic
    Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Stevia
    plain_language
    Without the activating calcium signal, potentiation did not turn the channel on.
    primary_references
    Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19) · lines 194–200

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Intracellular calcium buffered with BAPTA; whole-cell recordings. · source_derived_draft · unverified_draft

    ## stevia-calcium-gate Without the activating calcium signal, potentiation did not turn the channel on. Buffering intracellular free calcium prevented stevioside from activating TRPM5 in the expression assay. Model: Intracellular calcium buffered with BAPTA; whole-cell recordings. Limitations: This is a local signaling requirement, not evidence of dietary calcium deficiency or benefit from a calcium supplement. Evidence access: Primary full text; Figure 1d and methods Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733
    Complete structured claim and evidence

  2. Rebaudioside A increased calcium-activated TRPM5 currents in the expression system.

    Rebaudioside A → TRPM5 channel in the 2017 expression assay, construct species not independently verified source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text; Figure 1
    experimental_model
    HEK293T channel-expression patch clamp.
    limitations
    This direct bath exposure is not an oral human exposure study.
    nutrient_topic
    Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Stevia
    plain_language
    A second glycoside reaches the same channel endpoint.
    primary_references
    Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733

    Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19) · lines 178–184

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · HEK293T channel-expression patch clamp. · source_derived_draft · unverified_draft

    ## stevia-trpm5-reba A second glycoside reaches the same channel endpoint. Rebaudioside A increased calcium-activated TRPM5 currents in the expression system. Model: HEK293T channel-expression patch clamp. Limitations: This direct bath exposure is not an oral human exposure study. Evidence access: Primary full text; Figure 1 Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733
    Complete structured claim and evidence

  3. Stevioside increased calcium-activated TRPM5 currents in transfected HEK293T cells; the effect reversed with washout.

    Stevioside → TRPM5 channel in the 2017 expression assay, construct species not independently verified source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text; Figure 1 and patch-clamp methods
    experimental_model
    Whole-cell patch clamp with 1 micromolar free intracellular calcium.
    limitations
    Human host-cell origin does not establish construct species; that uncertainty is explicit in the channel node.
    nutrient_topic
    Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Stevia
    plain_language
    It strengthens an existing ion-channel response rather than supplying the calcium signal.
    primary_references
    Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733

    Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19) · lines 170–176

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Whole-cell patch clamp with 1 micromolar free intracellular calcium. · source_derived_draft · unverified_draft

    ## stevia-trpm5-stev It strengthens an existing ion-channel response rather than supplying the calcium signal. Stevioside increased calcium-activated TRPM5 currents in transfected HEK293T cells; the effect reversed with washout. Model: Whole-cell patch clamp with 1 micromolar free intracellular calcium. Limitations: Human host-cell origin does not establish construct species; that uncertainty is explicit in the channel node. Evidence access: Primary full text; Figure 1 and patch-clamp methods Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733
    Complete structured claim and evidence

  4. Steviol also potentiated calcium-activated TRPM5 currents, showing that the aglycone retains activity in this assay.

    Steviol → TRPM5 channel in the 2017 expression assay, construct species not independently verified source_derived_draftungraded
    Experimental context and source evidence
    evidence_access
    Primary full text; Figure 1 and supplementary-patch description
    experimental_model
    Whole-cell and excised-patch experiments.
    limitations
    Activity of steviol does not prove equivalent activity of its glucuronide at TRPM5.
    nutrient_topic
    Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit. · Stevia
    plain_language
    Removing sugar groups does not eliminate this channel effect.
    primary_references
    Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733

    Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19) · lines 186–192

    AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text. · supports · Whole-cell and excised-patch experiments. · source_derived_draft · unverified_draft

    ## stevia-trpm5-steviol Removing sugar groups does not eliminate this channel effect. Steviol also potentiated calcium-activated TRPM5 currents, showing that the aglycone retains activity in this assay. Model: Whole-cell and excised-patch experiments. Limitations: Activity of steviol does not prove equivalent activity of its glucuronide at TRPM5. Evidence access: Primary full text; Figure 1 and supplementary-patch description Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards