{"id":"2d888d14-fa86-5159-9c00-fe8ed07e9dd6","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-calcium-gate","predicate":"is_required_in_assay_for","statement":"Buffering intracellular free calcium prevented stevioside from activating TRPM5 in the expression assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1dd2667f-48e0-53d0-92da-4e52ee310ef3","mechanism_event_label":"Without the activating calcium signal, potentiation did not turn the channel on.","subject":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"object":{"id":"1fe12b9d-3f8c-554c-bafa-17d36facd810","slug":"trpm5-assay-channel","display_name":"TRPM5 channel in the 2017 expression assay, construct species not independently verified","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"1dd2667f-48e0-53d0-92da-4e52ee310ef3","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-calcium-gate-event","event_type":"observed_relationship","label":"Without the activating calcium signal, potentiation did not turn the channel on.","description":"Buffering intracellular free calcium prevented stevioside from activating TRPM5 in the expression assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1fe12b9d-3f8c-554c-bafa-17d36facd810","slug":"trpm5-assay-channel","display_name":"TRPM5 channel in the 2017 expression assay, construct species not independently verified","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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