{"id":"9bca0ea3-6e81-5c05-9de2-1d6d22e3d52b","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-trpm5-stev","predicate":"potentiates","statement":"Stevioside increased calcium-activated TRPM5 currents in transfected HEK293T cells; the effect reversed with washout.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"94ef85d6-61f1-5d3e-8a07-a5e2fe8134e4","mechanism_event_label":"It strengthens an existing ion-channel response rather than supplying the calcium signal.","subject":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"object":{"id":"1fe12b9d-3f8c-554c-bafa-17d36facd810","slug":"trpm5-assay-channel","display_name":"TRPM5 channel in the 2017 expression assay, construct species not independently verified","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"94ef85d6-61f1-5d3e-8a07-a5e2fe8134e4","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-trpm5-stev-event","event_type":"observed_relationship","label":"It strengthens an existing ion-channel response rather than supplying the calcium signal.","description":"Stevioside increased calcium-activated TRPM5 currents in transfected HEK293T cells; 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Figure 1 and patch-clamp methods","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Whole-cell patch clamp with 1 micromolar free intracellular calcium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human host-cell origin does not establish construct species; that uncertainty is explicit in the channel node.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"It strengthens an existing ion-channel response rather than supplying the calcium signal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Steviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4fe67f80-88f1-5085-b8bb-a4859b9346f5","evidence_kind":"source_excerpt","locator":"Lines 170-176","start_line":170,"end_line":176,"excerpt":"## stevia-trpm5-stev\nIt strengthens an existing ion-channel response rather than supplying the calcium signal.\nStevioside increased calcium-activated TRPM5 currents in transfected HEK293T cells; the effect reversed with washout.\nModel: Whole-cell patch clamp with 1 micromolar free intracellular calcium.\nLimitations: Human host-cell origin does not establish construct species; that uncertainty is explicit in the channel node.\nEvidence access: Primary full text; Figure 1 and patch-clamp methods\nSteviol glycosides enhance pancreatic beta-cell function and taste sensation by potentiation of TRPM5 channel activity. · 2017 · https://pubmed.ncbi.nlm.nih.gov/28361903/ · DOI 10.1038/ncomms14733","model_system":"Whole-cell patch clamp with 1 micromolar free intracellular calcium.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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