Component

Cellular retinaldehyde-binding protein / CRALBP

RLBP1 product binding cis-retinoids; same canonical protein in RPE and Müller glia.

4 recorded relationships. Experimental role, claim status and evidence remain attached to each record.

Read recorded mechanisms Open recursive mind map

How nutrients influence it

Every nutrient with a recorded effect on this component, credited to the nutrient that acted rather than the chapter that recorded it. Open a nutrient to see the findings and the conditions they were measured under.

How nutrients reach it in more than one step

Chains of two or more recorded steps that end here, grouped by the nutrient they start from. Each step is a separate finding, so a chain is a route a mechanism could take, not proof that it does.

Tracing routes…

What it does

Every recorded relationship this component is part of, grouped by its role. Plain wording comes first; the technical statement follows.

Recorded relationships

What it acts on

  1. CRALBP coexpression increased 11-cis-retinol production approximately threefold in homogenates of cells stably expressing chicken DES1.

    Cellular retinaldehyde-binding protein / CRALBP → 11-cis-retinol source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    evidence_location
    Figure 5b
    experimental_model
    Chicken CRALBP transfection into stable DES1-expressing 293T cells
    exposure
    All-trans-retinol substrate; CRALBP plasmid versus empty-vector comparison.
    limitations
    An assay yield effect does not prove physiological transport or human supplementation benefit.
    nutrient
    Vitamin A · Vitamin A
    nutrient_topic
    Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
    organism
    Gallus gallus proteins in Homo sapiens cells
    plain_language
    The binding protein shifted the measured output toward the visual-cycle alcohol.
    primary_references
    [vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. (2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114
    tissue_or_cell_type
    cell homogenates

    Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17) · lines 573–586

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Chicken CRALBP transfection into stable DES1-expressing 293T cells · source_derived_draft · unverified_draft

    ### vae-cralbp-des1-isomer-yield CRALBP coexpression increased 11-cis-retinol production approximately threefold in homogenates of cells stably expressing chicken DES1. Condition category: normal nutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. plain_language: The binding protein shifted the measured output toward the visual-cycle alcohol. organism: Gallus gallus proteins in Homo sapiens cells tissue_or_cell_type: cell homogenates experimental_model: Chicken CRALBP transfection into stable DES1-expressing 293T cells limitations: An assay yield effect does not prove physiological transport or human supplementation benefit. exposure: All-trans-retinol substrate; CRALBP plasmid versus empty-vector comparison. cross_nutrient: false evidence_location: Figure 5b nutrient: Vitamin A [vav-kaylor2013] Identification of DES1 as a vitamin A isomerase in Müller glial cells of the retina. (2013). https://pubmed.ncbi.nlm.nih.gov/23143414/ DOI: 10.1038/nchembio.1114
    Complete structured claim and evidence

  2. Adding native CRALBP increased the accumulation of light-generated 11-cis-retinal in bovine RPE microsomal assays compared with denatured CRALBP.

    Cellular retinaldehyde-binding protein / CRALBP → 11-cis-retinal source_derived_draftungraded
    Experimental context and source evidence
    cross_nutrient
    false
    evidence_location
    Results: Impact of CRALBP on the RPE photoisomerase activity; Figure 3d
    experimental_model
    Native versus denatured CRALBP in RPE microsomes
    exposure
    530-nm illumination; retinal substrate; Figure 3d comparison.
    limitations
    Product protection was inferred from yield; these assays do not establish supplement effects.
    nutrient
    Vitamin A · Vitamin A
    nutrient_topic
    Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
    organism
    Bos taurus; recombinant human CRALBP reagent
    plain_language
    The retinoid-binding protein helps retain RGR pathway product.
    primary_references
    [vav-zhang2019] Photic generation of 11-cis-retinal in bovine retinal pigment epithelium. (2019). https://pubmed.ncbi.nlm.nih.gov/31694912/ DOI: 10.1074/jbc.ra119.011169
    tissue_or_cell_type
    RPE microsomes

    Vitamin A expansion: reproduction, light-driven vision and shared mechanisms (2026-09-17) · lines 438–451

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Native versus denatured CRALBP in RPE microsomes · source_derived_draft · unverified_draft

    ### vae-cralbp-rgr-product-accumulation Adding native CRALBP increased the accumulation of light-generated 11-cis-retinal in bovine RPE microsomal assays compared with denatured CRALBP. Condition category: normal nutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect. plain_language: The retinoid-binding protein helps retain RGR pathway product. organism: Bos taurus; recombinant human CRALBP reagent tissue_or_cell_type: RPE microsomes experimental_model: Native versus denatured CRALBP in RPE microsomes limitations: Product protection was inferred from yield; these assays do not establish supplement effects. exposure: 530-nm illumination; retinal substrate; Figure 3d comparison. cross_nutrient: false evidence_location: Results: Impact of CRALBP on the RPE photoisomerase activity; Figure 3d nutrient: Vitamin A [vav-zhang2019] Photic generation of 11-cis-retinal in bovine retinal pigment epithelium. (2019). https://pubmed.ncbi.nlm.nih.gov/31694912/ DOI: 10.1074/jbc.ra119.011169
    Complete structured claim and evidence

  3. Müller-cell-directed CRALBP restoration in Rlbp1-null/Gnat1-null mice improved cone recovery in the isolated-retina paradigm.

    Cellular retinaldehyde-binding protein / CRALBP → Cone dark adaptation source_derived_draftungraded
    Experimental context and source evidence
    experimental_model
    Cell-directed AAV rescue; ex vivo cone ERG
    intervention
    AAV protein restoration in genetic knockout
    limitations
    Rescue from global deletion is not equivalent to deleting only Müller CRALBP in an intact eye.
    nutrient_topic
    Vitamin A research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
    organism
    Mus musculus
    plain_language
    Müller-cell CRALBP can support a retinal route for cone pigment renewal.
    primary_references
    [xue-2015] CRALBP supports the mammalian retinal visual cycle and cone vision (2015). https://pmc.ncbi.nlm.nih.gov/articles/PMC4319437/ DOI: 10.1172/JCI79651
    tissue_or_cell_type
    Müller glia and cone retina

    Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17) · lines 882–892

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Cell-directed AAV rescue; ex vivo cone ERG · source_derived_draft · unverified_draft

    ### a-vision-muller-cralbp-cones Müller-cell-directed CRALBP restoration in Rlbp1-null/Gnat1-null mice improved cone recovery in the isolated-retina paradigm. Condition category: normal nutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect. plain_language: Müller-cell CRALBP can support a retinal route for cone pigment renewal. organism: Mus musculus tissue_or_cell_type: Müller glia and cone retina experimental_model: Cell-directed AAV rescue; ex vivo cone ERG limitations: Rescue from global deletion is not equivalent to deleting only Müller CRALBP in an intact eye. intervention: AAV protein restoration in genetic knockout [xue-2015] CRALBP supports the mammalian retinal visual cycle and cone vision (2015). https://pmc.ncbi.nlm.nih.gov/articles/PMC4319437/ DOI: 10.1172/JCI79651
    Complete structured claim and evidence

Where it participates (unsigned role)

  1. RPE-selective CRALBP deletion slowed bulk visual chromophore regeneration approximately 15-fold in mice.

    RPE-selective CRALBP loss → 11-cis-retinal regeneration source_derived_draftungraded
    Experimental context and source evidence
    availability_state
    machinery_impairment Imported condition classification; unverified.
    experimental_model
    Timed RPE-selective knockout; post-bleach HPLC
    limitations
    Bulk recovery mainly reflects rods; not a direct human effect size.
    nutrient_topic
    Vitamin A research collection; topical membership is not evidence of a direct dietary effect. · Vitamin A
    organism
    Mus musculus
    plain_language
    RPE CRALBP strongly supports replenishment of cis retinal.
    primary_references
    [bassetto-2024] Dominant role for pigment epithelial CRALBP in supplying visual chromophore to photoreceptors (2024). https://pubmed.ncbi.nlm.nih.gov/38676924/ DOI: 10.1016/j.celrep.2024.114143
    tissue_or_cell_type
    RPE/whole-eye retinoids
    trigger_kind
    machinery_impairment Imported condition classification; unverified.

    Vitamin A: forms, mechanisms, deficiency and excess (2026-09-17) · lines 860–869

    AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text. · supports · Timed RPE-selective knockout; post-bleach HPLC · source_derived_draft · unverified_draft

    ### a-vision-rpe-cralbp-chromophore RPE-selective CRALBP deletion slowed bulk visual chromophore regeneration approximately 15-fold in mice. Condition category: machinery_impairment nutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect. plain_language: RPE CRALBP strongly supports replenishment of cis retinal. organism: Mus musculus tissue_or_cell_type: RPE/whole-eye retinoids experimental_model: Timed RPE-selective knockout; post-bleach HPLC limitations: Bulk recovery mainly reflects rods; not a direct human effect size. [bassetto-2024] Dominant role for pigment epithelial CRALBP in supplying visual chromophore to photoreceptors (2024). https://pubmed.ncbi.nlm.nih.gov/38676924/ DOI: 10.1016/j.celrep.2024.114143
    Complete structured claim and evidence

In the sources

Preserved passages that mention this component, quoted exactly. Open one to read it in context.

    This is a research prototype built from draft material. It is not medical advice, and its statements still await verification against the original studies.

    Evidence, AI assistance and curation standards