{"id":"11c71b4c-f973-5148-9f0a-ed1a52520368","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-cralbp-rgr-product-accumulation","predicate":"increases_accumulation_of","statement":"Adding native CRALBP increased the accumulation of light-generated 11-cis-retinal in bovine RPE microsomal assays compared with denatured CRALBP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"b98ac969-a840-5a9f-9b8b-b0b96bab5a4e","mechanism_event_label":"The retinoid-binding protein helps retain RGR pathway product.","subject":{"id":"fd923f7d-57da-58da-ad04-adb75072433f","slug":"rlbp1","display_name":"Cellular retinaldehyde-binding protein / CRALBP","entity_type_key":"protein"},"object":{"id":"2df0d238-71af-5f0b-8359-cef18235c4af","slug":"11-cis-retinal","display_name":"11-cis-retinal","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b98ac969-a840-5a9f-9b8b-b0b96bab5a4e","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:vae-cralbp-rgr-product-accumulation-event","event_type":"biochemical_relationship","label":"The retinoid-binding protein helps retain RGR pathway product.","description":"Adding native CRALBP increased the accumulation of light-generated 11-cis-retinal in bovine RPE microsomal assays compared with denatured CRALBP.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fd923f7d-57da-58da-ad04-adb75072433f","slug":"rlbp1","display_name":"Cellular retinaldehyde-binding protein / CRALBP","entity_type_key":"protein"},"role":"product-binding protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"205eda81-4688-5fa3-a08c-e94d0c1cbf5c","slug":"rgr","display_name":"Retinal G protein-coupled receptor / RGR opsin","entity_type_key":"protein"},"role":"microsomal photoisomerase","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2df0d238-71af-5f0b-8359-cef18235c4af","slug":"11-cis-retinal","display_name":"11-cis-retinal","entity_type_key":"small_molecule"},"role":"measured product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Results: Impact of CRALBP on the RPE photoisomerase activity; Figure 3d","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Native versus denatured CRALBP in RPE microsomes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"530-nm illumination; retinal substrate; Figure 3d comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Product protection was inferred from yield; these assays do not establish supplement effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient","value_text":"Vitamin A","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Bos taurus; recombinant human CRALBP reagent","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The retinoid-binding protein helps retain RGR pathway product.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vav-zhang2019] Photic generation of 11-cis-retinal in bovine retinal pigment epithelium. (2019). https://pubmed.ncbi.nlm.nih.gov/31694912/ DOI: 10.1074/jbc.ra119.011169","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"RPE microsomes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"60923904-06eb-59c7-88d1-280e989afcbe","evidence_kind":"source_excerpt","locator":"Lines 438-451","start_line":438,"end_line":451,"excerpt":"### vae-cralbp-rgr-product-accumulation\nAdding native CRALBP increased the accumulation of light-generated 11-cis-retinal in bovine RPE microsomal assays compared with denatured CRALBP.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The retinoid-binding protein helps retain RGR pathway product.\norganism: Bos taurus; recombinant human CRALBP reagent\ntissue_or_cell_type: RPE microsomes\nexperimental_model: Native versus denatured CRALBP in RPE microsomes\nlimitations: Product protection was inferred from yield; these assays do not establish supplement effects.\nexposure: 530-nm illumination; retinal substrate; Figure 3d comparison.\ncross_nutrient: false\nevidence_location: Results: Impact of CRALBP on the RPE photoisomerase activity; Figure 3d\nnutrient: Vitamin A\n[vav-zhang2019] Photic generation of 11-cis-retinal in bovine retinal pigment epithelium. 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